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AB313853

Anti-ErbB2 / HER2 (phospho Y1248) antibody [HL1466] - BSA and Azide free

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Rabbit Recombinant Monoclonal ErbB2 / HER2 phospho Y1248 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Human samples. Immunogen corresponding to Synthetic Peptide within Human ERBB2 pY1248 conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

CD340, HER2, MLN19, NEU, NGL, ERBB2, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, Tyrosine kinase-type cell surface receptor HER2, p185erbB2, MLN 19, CD340, Neu, Erbb2, Receptor tyrosine-protein kinase erbB-2, Epidermal growth factor receptor-related protein, Proto-oncogene Neu, Proto-oncogene c-ErbB-2, p185erbB2, p185neu

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y1248) antibody [HL1466] - BSA and Azide free (AB313853)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y1248) antibody [HL1466] - BSA and Azide free (AB313853)

Immunohistochemical analysis of paraffin-embedded rat testis and lung tissue labeling ErbB2 / HER2 (phospho Y1248) with ab313853 at 1/100 dilution. Antigen retrieval performed using Citrate buffer pH 6.0 for 15 minutes.

Western blot - Anti-ErbB2 / HER2 (phospho Y1248) antibody [HL1466] - BSA and Azide free (AB313853)
  • WB

Supplier Data

Western blot - Anti-ErbB2 / HER2 (phospho Y1248) antibody [HL1466] - BSA and Azide free (AB313853)

All lanes:

Western blot - Anti-ErbB2 / HER2 (phospho Y1248) antibody [HL1466] - BSA and Azide free (ab313853) at 1/1000 dilution

Lane 1:

Untreated starved (16 hr) A431 (Human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 2:

EGF treated (100 ng/mL, 5 min) starved (16 hr) A431 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 138 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1466

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ERBB2 pY1248 conjugated to Keyhole Limpet Haemocyanin.

P04626

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보완 제품

Primary Antibodies

AB237715

Anti-ErbB2 / HER2 antibody [CAL27]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 antibody [CAL27] (AB237715)

  • 0
  • 0
Primary Antibodies

AB52894

Anti-EGFR antibody [EP38Y]

BOND RX™ Validated|KO Validated|RabMAb|Lab Essentials|20ul selling size|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR antibody [EP38Y] (AB52894)

  • 5
  • 24
Primary Antibodies

AB109273

Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)]

RabMAb|Recombinant

Western blot - Anti-ErbB4 / HER4 (phospho Y1284) antibody [EPR2273(2)] (AB109273)

  • 5
  • 1
Primary Antibodies

AB201688

Anti-CSDE1/NRU antibody [EPR17414]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CSDE1/NRU antibody [EPR17414] (AB201688)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The ErbB2 also known as HER2 or HER2 protein is a transmembrane receptor protein with a molecular weight of about 185 kDa. It serves as part of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. The ErbB2 protein is expressed mainly in epithelial tissues including those of the breast and the gastrointestinal tract. It lacks a known ligand-binding domain which sets it apart from other members of its family. Due to its structure ErbB2 dimerizes with other members of the EGFR family to exert its effects in cellular signaling.
Biological function summary

The role of ErbB2 extends beyond a singular function. It becomes an active component when forming heterodimers with other EGFR family members such as ErbB3 to initiate various downstream signaling cascades. The dimerization activates intracellular pathways leading to cell proliferation survival differentiation and migration. Within cells ErbB2 influences processes critical for normal development and tissue homeostasis contributing significantly to signal transduction networks.

Pathways

ErbB2 plays a pivotal role in pathways such as the PI3K/Akt and MAPK/ERK pathways. These pathways are important in mediating cellular responses to growth signals. The PI3K/Akt pathway activated by HER2 signaling regulates cell growth and survival while the MAPK/ERK pathway contributes to cell differentiation and proliferation. ErbB2's interaction with proteins like ErbB3 is fundamental in these pathways increasing the amplitude and diversity of downstream signals.

ErbB2 is significantly associated with breast cancer and gastric cancer. The overexpression or gene amplification of HER2 is observed in approximately 20% of breast cancer cases correlating with aggressive tumor growth and poor prognosis. ErbB2-related signaling contributes to oncogenic processes by promoting excessive cell proliferation. Targeting ErbB2 in these cancers is common using therapies such as monoclonal antibodies like trastuzumab. In the context of gastric cancer the role of ErbB2 mirrors its function in breast cancer and targeting HER2 holds therapeutic potential.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.. In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
See full target information ERBB2 pY1248
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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