AB230540

Anti-ERO1LB antibody - C-terminal

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Rabbit Polyclonal ERO1LB antibody. C-terminal. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ERO1B aa 250-300 conjugated to Keyhole Limpet Haemocyanin.

대체 명칭 보기

ERO1LB, ERO1B, ERO1-like protein beta, ERO1-L-beta, Endoplasmic reticulum oxidoreductase beta, Endoplasmic reticulum oxidoreductin-1-like protein B, Oxidoreductin-1-L-beta

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERO1LB antibody - C-terminal (AB230540)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERO1LB antibody - C-terminal (AB230540)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for ERO1LB using ab230540 at 1/50 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-ERO1LB antibody - C-terminal (AB230540)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ERO1LB antibody - C-terminal (AB230540)

Intracellular flow cytometric analysis of MDA-MB-435 cell line labeling ERO1LB with ab230540 at 1/10 dilution (green) compared with a negative control (blue).

Supplier Data

Western blot - Anti-ERO1LB antibody - C-terminal (AB230540)

All lanes:

Western blot - Anti-ERO1LB antibody - C-terminal (ab230540) at 1/1000 dilution

All lanes:

MDA-MB435 whole cell lysate at 35 µg

Predicted band size: 54 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

Synthetic Peptide within Human ERO1B aa 250-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q86YB8

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ERO1LB also known as Endoplasmic Reticulum Oxidoreductin 1 Beta or ERO1-like protein beta is an enzyme involved in oxidative protein folding. It is approximately 55 kDa in size. ERO1LB localizes mainly in the endoplasmic reticulum and is expressed in various tissues with higher expression in the pancreas and secretory tissues. This protein functions by reoxidizing PDI (protein disulfide isomerase) enzymes which assists in forming disulfide bonds essential for protein folding.

Biological function summary

ERO1LB plays a role in the cellular response to oxidative stress and regulation of the redox state. It acts as part of a complex that maintains the proper folding of secretory and membrane proteins. ERO1LB helps maintain the balance between reducing equivalents in the endoplasmic reticulum and cellular homeostasis. It contributes to efficient protein secretion which is critical for tissues with high secretory demands.

Pathways

ERO1LB is integral to the protein folding and secretory pathways. It participates in the oxidative folding pathway by regenerating the oxidized state of PDIs which directly impacts protein disulfide formation. ERO1LB interacts with proteins such as PDI and ERO1A to maintain redox homeostasis ensuring the efficacy of the protein folding machinery. It helps regulate the unfolded protein response (UPR) a pathway important for preventing the accumulation of misfolded proteins.

Oxidative stress-related conditions and pancreatic diseases involve the ERO1LB protein. Abnormal expression of ERO1LB can contribute to disorders like diabetes given its role in insulin biosynthesis. The protein interacts with insulin processing and secretion where a misbalance might result in disease. Additionally ERO1LB connects to oxidative stress mechanisms linking to the pathology of neurodegenerative diseases through shared pathways with other oxidoreductases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein disulfide isomerase family members can also be reoxidized, but at lower rates compared to P4HB, including PDIA2 (50% of P4HB reoxidation rate), as well as PDIA3, PDIA4, PDIA6 and NXNDC12 (<10%). Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. May be involved in oxidative proinsulin folding in pancreatic cells, hence may play a role in glucose homeostasis.

See full target information ERO1B

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nucleic acids research 52:3778-3793 PubMed38348929

2024

Human CST complex restricts excessive PrimPol repriming upon UV induced replication stress by suppressing p21.

Applications

Unspecified application

Species

Unspecified reactive species

Pau Biak Sang,Rishi K Jaiswal,Xinxing Lyu,Weihang Chai

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ERO1LB antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERO1LB antibody - C-terminal (AB230540)

Flow Cytometry (Intracellular) - Anti-ERO1LB antibody - C-terminal (AB230540)

Western blot - Anti-ERO1LB antibody - C-terminal (AB230540)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise