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AB242367

Anti-ERp57 antibody [CL2444]

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Mouse Monoclonal ERp57 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PDIA3 aa 100-250.
10 이미지
Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)

Immunofluorescence/immunocytochemical analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling ERp57 (Green) using ab242367 at 4 μg/mL. The nuclear counterstain is DAPI (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)

Immunofluorescence/immunocytochemical analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ERp57 (Green) using ab242367 at 4 μg/mL. The nuclear counterstain is DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)

Paraffin-embedded human cerebral cortex tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)

Immunofluorescence/immunocytochemical analysis of A431 (human epidermoid carcinoma cell line) cells labeling ERp57 (Green) using ab242367 at 4 μg/mL. The nuclear counterstain is DAPI (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)

Immunofluorescence/immunocytochemical analysis of U-251 MG (Human brain glioma cell line) cells labeling ERp57 (Green) using ab242367 at 4 μg/mL. The nuclear counterstain is DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)

Negative control :

Paraffin-embedded human skeletal muscle tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)

Paraffin-embedded human thyroid gland tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [CL2444] (AB242367)

Paraffin-embedded human endometrium tissue stained for ERp57 using ab242367 at 1/5000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [CL2444] (AB242367)

Immunofluorescence/immunocytochemical analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling ERp57 (Green) using ab242367 at 4 μg/mL. The nuclear counterstain is DAPI (Blue).

Western blot - Anti-ERp57 antibody [CL2444] (AB242367)
  • WB

Supplier Data

Western blot - Anti-ERp57 antibody [CL2444] (AB242367)

All lanes:

Western blot - Anti-ERp57 antibody [CL2444] (ab242367)

Predicted band size: 57 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL2444

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PDIA3 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

P30101

Epitope

Binds to an epitope located within the peptide sequence KKFISDKDASIVGFF as determined by overlapping synthetic peptides.

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대체품

Primary Antibodies

AB10287

Anti-ERp57 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (AB10287)

  • 5
  • 2
Primary Antibodies

AB13506

Anti-ERp57 antibody [MaP.Erp57]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody [MaP.Erp57] (AB13506)

  • 5
  • 7
Primary Antibodies

AB154191

Anti-ERp57 antibody [EPR10678(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10678(B)] (AB154191)

  • 0
  • 0
Primary Antibodies

AB154197

Anti-ERp57 antibody [EPR10679(B)]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERp57 antibody [EPR10679(B)] (AB154197)

  • 0
  • 0
Primary Antibodies

AB183396

FITC Anti-ERp57 antibody [Map.ERP57]

  • 0
  • 0

보완 제품

Primary Antibodies

AB21685

Anti-GRP78 BiP antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (AB21685)

  • 5
  • 71
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB92516

Anti-Calreticulin antibody [EPR3924] - ER Marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calreticulin antibody [EPR3924] - ER Marker (AB92516)

  • 4
  • 6
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
Purification 관련 사항
Purified from TCS.
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ERp57 also known as 4f9 is a protein responsible for catalyzing disulfide bond formations and isomerizations in the endoplasmic reticulum. ERp57 has a molecular mass of approximately 57 kDa and belongs to the protein disulfide isomerase (PDI) family. Its main expression occurs in the endoplasmic reticulum of cells where it plays a role in ensuring proper protein folding and quality control.
Biological function summary

ERp57 interacts with calnexin and calreticulin to form a complex involved in glycoprotein folding. This complex assists in the proper assembly and maturation of nascent glycoproteins by ensuring correct folding pathways within the cell. ERp57 has a specialized role in managing oxidative protein folding which makes it essential for cell function and homeostasis.

Pathways

ERp57 participates in the calnexin/calreticulin cycle which is critical for protein triage in the endoplasmic reticulum. This cycle ensures that only properly folded proteins progress to their final destinations. ERp57 also interacts with ERp72 another member of the PDI family to facilitate thiol-disulfide exchange reactions. These interactions position ERp57 as a central player in the maintenance of cellular redox balance and protein homeostasis.

Alterations in ERp57 function can relate to neurodegenerative diseases such as Alzheimer's and certain types of cancer. Misfolded proteins often accumulate in these conditions highlighting ERp57's role in preventing cellular stress. Moreover ERp57 associates with the protein tau in Alzheimer's disease where it assists in tau’s proper folding and diminishes its aggregation tendency. Additionally disruptions in the ERp57 pathway may interact with cancer-related proteins like p53 modulating apoptosis and cell cycle regulation.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Protein disulfide isomerase that catalyzes the formation, isomerization, and reduction or oxidation of disulfide bonds in client proteins and functions as a protein folding chaperone (PubMed : 11825568, PubMed : 16193070, PubMed : 27897272, PubMed : 36104323, PubMed : 7487104). Core component of the major histocompatibility complex class I (MHC I) peptide loading complex where it functions as an essential folding chaperone for TAPBP. Through TAPBP, assists the dynamic assembly of the MHC I complex with high affinity antigens in the endoplasmic reticulum. Therefore, plays a crucial role in the presentation of antigens to cytotoxic T cells in adaptive immunity (PubMed : 35948544, PubMed : 36104323).
See full target information PDIA3

대체 명칭 보기

ERP57, ERP60, GRP58, PDIA3, Protein disulfide-isomerase A3, 58 kDa glucose-regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER-60, Endoplasmic reticulum resident protein 57, Endoplasmic reticulum resident protein 60, p58, ER protein 57, ERp57, ER protein 60, ERp60
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nucleic acids research 49:8024-8036 PubMed34223906

2021

LSH catalyzes ATP-driven exchange of histone variants macroH2A1 and macroH2A2.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Ni,Kathrin Muegge
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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