JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB259430

Anti-Estrogen Receptor alpha antibody [ESR1/3557] - BSA and Azide free

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Mouse Monoclonal Estrogen Receptor alpha antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra), Protein Array, ICC/IF and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ESR1 aa 100-350.
5 이미지
Flow Cytometry - Anti-Estrogen Receptor alpha antibody [ESR1/3557] - BSA and Azide free (AB259430)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Estrogen Receptor alpha antibody [ESR1/3557] - BSA and Azide free (AB259430)

Flow cytometric analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling Estrogen Receptor alpha using ab259427 at 2 μg / 106 cells (blue) as compared to an isotype control (red).

The secondary antibody is a Goat anti-Mouse IgG CF488 conjugate.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab259427).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [ESR1/3557] - BSA and Azide free (AB259430)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [ESR1/3557] - BSA and Azide free (AB259430)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling Estrogen Receptor alpha using ab259427 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab259427).

Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [ESR1/3557] - BSA and Azide free (AB259430)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody [ESR1/3557] - BSA and Azide free (AB259430)

MCF7 (human breast adenocarcinoma cell line) cells labeling Estrogen Receptor alpha using ab259427 at 2 μg/ml (green) in ICC/IF.

The membrane is stained with Phalloidin (red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab259427).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [ESR1/3557] - BSA and Azide free (AB259430)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [ESR1/3557] - BSA and Azide free (AB259430)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling Estrogen Receptor alpha using ab259427 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab259427).

Protein Array - Anti-Estrogen Receptor alpha antibody [ESR1/3557] - BSA and Azide free (AB259430)
  • Protein Array

Ap

Protein Array - Anti-Estrogen Receptor alpha antibody [ESR1/3557] - BSA and Azide free (AB259430)

Protein array containing >19,000 full-length human proteins, probed with ab259427.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab259427).

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

ESR1/3557

Isotype

IgG1

Light chain 타입

kappa

Carrier free

Yes

Reacts with

Human

Applications

Protein Array, Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ESR1 aa 100-350. The exact immunogen used to generate this antibody is proprietary information.

P03372

style
left-column

대체품

Primary Antibodies

AB167610

Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free

Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Frozen sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] - BSA and Azide free (AB167610)

  • 5
  • 2
Primary Antibodies

AB167611

Anti-Estrogen Receptor alpha antibody [E115] - Low endotoxin, Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [E115] - Low endotoxin, Azide free (AB167611)

  • 0
  • 0
Primary Antibodies

AB32063

Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|Advanced Validation|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [E115] - ChIP Grade (AB32063)

  • 4
  • 21
Primary Antibodies

AB108398

Anti-Estrogen Receptor alpha antibody [EPR4097]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-Estrogen Receptor alpha antibody [EPR4097] (AB108398)

  • 5
  • 12
Primary Antibodies

AB32396

Anti-Estrogen Receptor alpha (phospho S118) antibody [E91]

20ul selling size|RabMAb|Recombinant|Lab Essentials|Advanced Validation|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] (AB32396)

  • 4
  • 2
Primary Antibodies

AB271827

Anti-Estrogen Receptor alpha antibody [E115] - BSA and Azide free

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [E115] - BSA and Azide free (AB271827)

  • 1
  • 1

보완 제품

Assay Kits

AB207203

Estrogen Receptor Transcription Factor Assay Kit (Colorimetric)

Functional Studies - Estrogen Receptor Transcription Factor Assay Kit (Colorimetric) (AB207203)

  • 0
  • 0
Primary Antibodies

AB16661

Anti-Progesterone Receptor antibody [SP2]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Anti-Progesterone Receptor antibody [SP2] (AB16661)

  • 4
  • 4
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB9498

Anti-CD31 antibody [JC/70A]

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [JC/70A] (AB9498)

  • 4
  • 40
Primary Antibodies

AB133273

Anti-Androgen Receptor antibody [EPR1535(2)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

  • 5
  • 12
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Primary incubation for 30 minutes at room temperature.</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

제품 세부 정보

ab259430 is the carrier-free version of ab259427.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Estrogen Receptor alpha also known as ERα or ESR1 is a nuclear receptor that acts as a transcription factor when activated by its ligand estrogen. Its molecular weight is approximately 66 kDa. ERα is expressed in various tissues such as breast tissue endometrium and ovarian cells as well as some areas of the central nervous system. The ERα protein binds to specific DNA sequences called estrogen response elements to regulate the transcription of target genes. Tools like ER alpha ELISA and assays using alpha peptides help to study this receptor.
Biological function summary

ERα plays a significant role in the regulation of estrogen signaling. Estrogens binding to ERα activate the receptor which can form a homodimer or heterodimer complex with other proteins like coactivators or corepressors. This complex then modulates the transcription of genes involved in cell growth proliferation and differentiation. ERα is closely linked with processes like reproductive tissue development and maintenance.

Pathways

ERα is involved in the estrogen signaling pathway and the cell cycle regulation pathway. In the estrogen signaling pathway ERα works together with proteins such as coactivators which enhance gene transcription and corepressors which can inhibit transcription. In the context of cell cycle regulation ERα's interactions with other cell cycle proteins help control cell division and proliferation linking ERα activity to the progression through different stages of the cell cycle.

ERα's dysregulation has been implicated in breast cancer and osteoporosis. ERα overexpression or mutations can lead to oncogenic effects in breast cancer making it a prominent therapeutic target. Drugs that modulate ERα activity like selective estrogen receptor modulators (SERMs) are used in breast cancer treatment. In osteoporosis ERα is related to bone density regulation with its activity affecting bone resorption and formation. Relationships between ERα and other proteins such as those involved in hormone signaling pathways impact these disease mechanisms.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed : 17922032). Maintains neuronal survival in response to ischemic reperfusion injury when in the presence of circulating estradiol (17-beta-estradiol/E2) (By similarity).. Isoform 3. Involved in activation of NOS3 and endothelial nitric oxide production (PubMed : 21937726). Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor (PubMed : 10970861). Binds to ERE and inhibits isoform 1 (PubMed : 10970861).
See full target information ESR1

대체 명칭 보기

ESR, NR3A1, ESR1, Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

Frontiers in medicine 9:841170 PubMed35252273

2022

Immune Cells Profiles in the Different Sites of COVID-19-Affected Lung Lobes in a Single Patient.

Applications

Unspecified application

Species

Unspecified reactive species

Sadahiro Iwabuchi,Kyohei Miyamoto,Mayuko Hatai,Yurina Mikasa,Masahiro Katsuda,Shin-Ichi Murata,Toshikazu Kondo,Hiroki Yamaue,Shinichi Hashimoto
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com