Anti-Estrogen Receptor antibody [ESR1/1935] - BSA and Azide free
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Mouse Monoclonal Estrogen Receptor alpha antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ESR1.
대체 명칭 보기
ESR, NR3A1, ESR1, Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [ESR1/1935] - BSA and Azide free (AB237837)
Formalin-fixed, paraffin-embedded human endometrial carcinoma tissue stained for Estrogen Receptor using ab237995 at 2 μg/ml in immunohistochemical analysis.
This data was produced with ab237995, the same antibody in a different formulation with BSA and Azide.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor antibody [ESR1/1935] - BSA and Azide free (AB237837)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Estrogen Receptor using ab237995 at 2 μg/ml in immunohistochemical analysis.
This data was produced with ab237995, the same antibody in a different formulation with BSA and Azide.
- WB
Unknown
Western blot - Anti-Estrogen Receptor antibody [ESR1/1935] - BSA and Azide free (AB237837)
This data was produced with ab237995, the same antibody in a different formulation with BSA and Azide.
All lanes:
Western blot - Anti-Estrogen Receptor antibody [ESR1/1935] - BSA and Azide free (ab237837) at 1 µg/mL
All lanes:
MCF7 (human breast adenocarcinoma cell line) cell lysate
Predicted band size: 66 kDa
false
- Protein Array
Unknown
Protein Array - Anti-Estrogen Receptor antibody [ESR1/1935] - BSA and Azide free (AB237837)
This data was produced with ab237995, the same antibody in a different formulation with BSA and Azide.
ab237995 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Reactivity 정보
제품 세부 정보
ab237837 is the carrier-free version of ab237995.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
특성 및 보관 정보
제형
Purification 테크닉
Purification 관련 사항
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
분주 정보
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The Estrogen Receptor functions as a transcription factor that regulates gene expression when it binds to its ligand which is commonly estradiol. Upon binding 17 beta estradiol the receptor dimerizes and interacts with specific DNA sequences to influence transcription. It significantly regulates genes responsible for cell growth differentiation and reproductive functions. The estradiol chemical structure allows for specific interactions with ER altering the transcriptional regulation in a ligand-dependent manner. Though functioning mainly as a monomer ER can form heterodimers with other nuclear receptors affecting its biological role.
Pathways
The Estrogen Receptor is an important player in the genomic signaling pathway where it modulates the expression of numerous target genes by direct DNA binding. It also participates in the non-genomic signaling pathway where it involves the activation of second messengers and kinases. ER closely associates with important proteins such as coactivators and corepressors in these pathways. Estradiol chemical formula interactions facilitate molecular events involving ER-alpha to influence components of cell proliferation and survival.
제품 프로토콜
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타겟 정보
제품이 사용된 논문 (1)
Recent publications for all applications. Explore the 전체 목록 and refine your search
iScience 25:105503 PubMed36404924
2022
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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