Anti-Estrogen receptor beta antibody [EPR27876-74]

• WB

Supplier Data

Western blot - Anti-Estrogen receptor beta antibody [EPR27876-74] (AB323621)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : LNCaP, HeLa.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Estrogen receptor beta antibody [EPR27876-74] (ab323621) at 1/1000 dilution

Lane 1:

Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 2:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 3:

LNCaP (human prostate carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 60 kDa,36 kDa

false

Exposure time: 180s

• Dot

Supplier Data

Dot Blot - Anti-Estrogen receptor beta antibody [EPR27876-74] (AB323621)

Dot blot analysis of Estrogen receptor beta using ab323621 at 1 : 1000 (0.512 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : 293T cells transfected with an empty vector containing a His-tag, whole cell lysate 250 ng
Lane2 : 293T cells transfected with a human ESR2 expression vector containing a His-tag, whole cell lysate 50 ng
Lane3 : 293T cells transfected with a human ESR1 expression vector containing a His-tag, whole cell lysate 250 ng

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human ESR1.

In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Dot Blot - Anti-Estrogen receptor beta antibody [EPR27876-74] (ab323621) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 250 ng

Lane 2:

293T cells transfected with a human ESR2 expression vector containing a His-tag, whole cell lysate at 50 ng

Lane 3:

293T cells transfected with a human ESR1 expression vector containing a His-tag, whole cell lysate at 250 ng

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s