![Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (AB243564)](https://content.abcam.com/products/images/estrone-antibody-epptx-r7-94-2-bsa-and-azide-free-ab243564--competitive-elisa-img47528.jpg/_jcr_content/renditions/webp-768.webp)
- cELISA
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Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (AB243564)
Goat anti-rabbit IgG was coated onto 96-wells. Serial dilution of Estrone-6 (0, 15, 30, 100, 250, 500, 1000, 2000pg/mL) was added (25μL). This was followed by adding of 50μL of HRP-conjugated Estrone and 2.0-2.5ng/mL ab241064 (50μL) into each well. Plates were incubated for 2 hours at room temperature by shaking. After washing, 125μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. Then 125μL of stopping solution (acid solution) was dispensed and OD was read at 450nm on a microplate reader within 5 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241064).
![Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (AB243564)](https://content.abcam.com/products/images/estrone-antibody-epptx-r7-94-2-bsa-and-azide-free-ab243564--competitive-elisa-img49913.jpg/_jcr_content/renditions/webp-768.webp)
- cELISA
Unknown
Competitive ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] - BSA and Azide free (AB243564)
ELISA - Anti-Estrone antibody [EPPTX-R7-94-2] (ab241064) at 2.0 ng/ml.
ab241064 is specific to Estrone-6 and the percent cross reactivity with other compounds is demonstrated in the table.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241064).
관련 conjugated 항체와 다양한 조성의 항체 (1)
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Anti-Estrone antibody [EPPTX-R7-94-2]
보완 제품
Primary Antibodies
AB312903
Alexa Fluor® 594 Anti-CD68 antibody [EPR23917-164]
BOND RX™ Validated|RabMAb|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-CD68 antibody [EPR23917-164] (AB312903)](https://content.abcam.com/products/images/alexa-fluor-594-cd68-antibody-epr23917-164-ab312903--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img417006.jpg)
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Reactivity 정보
제품 세부 정보
ab243564 is the carrier-free version of ab241064.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
특성 및 보관 정보
제형
Purification 테크닉
보관 버퍼
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
보관 정보
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Estrogen hormones like estrone play significant roles in feminine reproductive health and secondary sexual characteristics. Estrone maintains a balance through conversion to and from other estrogens within the body. It does not function as a complex but influences biological processes such as cell proliferation and tissue growth particularly in reproductive organs. By interacting with cellular mechanisms estrone significantly impacts various physiological responses.
Pathways
Steroid hormones such as estrone integrate into metabolic and reproductive pathways. In the biosynthesis of steroids estrone converts from androstenedione or estradiol through enzymatic actions. The estrogen signaling pathway involves estrone and proteins such as estrogen receptor alpha (ERα) and estrogen receptor beta (ERβ). ERα and ERβ modulate transcriptional activities in various estrogen-responsive genes.
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제품이 사용된 논문 (1)
Recent publications for all applications. Explore the 전체 목록 and refine your search
NPJ precision oncology 7:95 PubMed37723227
2023
Applications
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Species
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Product promise
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