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AB126305

Anti-Ext1 antibody

5

(2 리뷰들)

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(9 제품이 사용된 논문 )

Anti-Ext1 antibody (ab126305) is a rabbit polyclonal antibody detecting Ext1 in IHC-P. Suitable for Human.

대체 명칭 보기

Exostosin-1, Exostosin glycosyltransferase 1, Heparan sulfate co-polymerase subunit EXT1, Multiple exostoses protein 1, N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase, EXT1

4 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ext1 antibody (AB126305)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ext1 antibody (AB126305)

Immunohistochemical analysis of human prostate tissue labeling Ext1 in the cytoplasm of smooth muscle cells with ab126305 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ext1 antibody (AB126305)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ext1 antibody (AB126305)

Immunohistochemical analysis of human liver tissue labeling Ext1 in the cytoplasm of hepatocytes with ab126305 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ext1 antibody (AB126305)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ext1 antibody (AB126305)

Immunohistochemical analysis of human rectum tissue labeling Ext1 in the cytoplasm of glandular cells with ab126305 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ext1 antibody (AB126305)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ext1 antibody (AB126305)

Immunohistochemical analysis of human placenta tissue labeling Ext1 in the cytoplasm of trophoblastic cells with ab126305 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human EXT1 aa 200-350. The exact immunogen used to generate this antibody is proprietary information.

Q16394

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보완 제품

Primary Antibodies

AB48197

Anti-SEMA3B antibody

Western blot - Anti-SEMA3B antibody (AB48197)

  • 5
  • 2
Primary Antibodies

AB188028

Anti-PLA2R antibody [CL0474]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLA2R antibody [CL0474] (AB188028)

  • 0
  • 0
Primary Antibodies

AB243031

Anti-THSD7A antibody [CL3778]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-THSD7A antibody [CL3778] (AB243031)

  • 0
  • 0
Primary Antibodies

AB197315

Anti-NELL1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NELL1 antibody (AB197315)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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제품 세부 정보

What is this antibody validated in?
Anti-Ext1 antibody (ab126305) is a rabbit polyclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ext1 also known as Exostosin-1 functions as an enzyme that participates in the glycosyltransferase family. It has a mass of approximately 100 kDa. Ext1 plays a significant mechanical role in catalyzing the elongation of heparan sulfate chains. This process involves the transfer of sugar residues to a growing polysaccharide chain which is important for synthesizing heparan sulfate proteoglycans. Ext1 is expressed in various tissues including the brain kidney and cartilage.
Biological function summary

Ext1 contributes to the biosynthesis of heparan sulfate a significant sulfated polysaccharide in the extracellular matrix and cell surface. Heparan sulfate chains influence cell signaling and interaction processes important for cellular homeostasis development and repair. Ext1 operates as part of an enzyme complex with Ext2 another member of the exostosin family facilitating coordinated activity in heparan sulfate polymerization. This complex ensures the controlled addition of sugar residues necessary for proper chain formation.

Pathways

Ext1 plays a role in the heparan sulfate biosynthesis and the Hedgehog signaling pathway. In the context of heparan sulfate biosynthesis Ext1 modifies the structure of heparan sulfate chains that modulate various signaling pathways influencing processes such as cell proliferation and differentiation. In the Hedgehog pathway Ext1 interacts with proteins like Sonic Hedgehog (SHH) by contributing to the modification of the cellular microenvironment affecting signal transmission.

Ext1 mutations or abnormalities associate with hereditary multiple exostoses (HME) a condition characterized by the growth of exostoses or bone spurs. The defect in Ext1 disrupts normal heparan sulfate synthesis contributing to abnormal skeletal development in HME. Additionally abnormalities in Ext1 exhibit connections to osteochondroma formation. The dysregulation of Ext1 may also influence the activity of other proteins such as Ext2 as these proteins work closely in glycosaminoglycan biosynthesis affecting disease progression and phenotype expression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Glycosyltransferase forming with EXT2 the heterodimeric heparan sulfate polymerase which catalyzes the elongation of the heparan sulfate glycan backbone (PubMed : 10639137, PubMed : 22660413, PubMed : 36402845, PubMed : 36593275, PubMed : 9620772). Glycan backbone extension consists in the alternating transfer of (1->4)-beta-D-GlcA and (1->4)-alpha-D-GlcNAc residues from their respective UDP-sugar donors. Both EXT1 and EXT2 are required for the full activity of the polymerase since EXT1 bears the N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity within the complex while EXT2 carries the glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity (PubMed : 36402845, PubMed : 36593275). Heparan sulfate proteoglycans are ubiquitous components of the extracellular matrix and play an important role in tissue homeostasis and signaling (PubMed : 10639137, PubMed : 11391482, PubMed : 22660413, PubMed : 9620772).
See full target information EXT1
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제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Glomerular diseases 5:176-190 PubMed40303503

2025

Diagnostic Immunostaining of Renal Biopsies: An Overview of Markers for Glomerular Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Vinita Agrawal,Alok Sharma

Kidney international reports 9:1040-1046 PubMed38765564

2024

Glomerular Exostosin-Positivity is Associated With Disease Activity and Outcomes in Patients With Membranous Lupus Nephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Xia,Suchun Li,Zhipeng Wang,Siyang Ye,Yuting Fan,Wenxing Peng,Wenfang Chen,Fengxian Huang,Ruihan Tang,Wei Chen

PloS one 18:e0281820 PubMed36809261

2023

Conditional ablation of heparan sulfate expression in stromal fibroblasts promotes tumor growth in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Ayumi Niwa,Toshiaki Taniguchi,Hiroyuki Tomita,Hideshi Okada,Takamasa Kinoshita,Chika Mizutani,Mikiko Matsuo,Yuko Imaizumi,Takahito Kuroda,Koki Ichihashi,Takaaki Sugiyama,Tomohiro Kanayama,Yu Yamaguchi,Shigeyuki Sugie,Nobuhisa Matsuhashi,Akira Hara

Science advances 7: PubMed33962942

2021

Alternative glycosylation controls endoplasmic reticulum dynamics and tubular extension in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Despoina Kerselidou,Bushra Saeed Dohai,David R Nelson,Sarah Daakour,Nicolas De Cock,Zahra Al Oula Hassoun,Dae-Kyum Kim,Julien Olivet,Diana C El Assal,Ashish Jaiswal,Amnah Alzahmi,Deeya Saha,Charlotte Pain,Filip Matthijssens,Pierre Lemaitre,Michael Herfs,Julien Chapuis,Bart Ghesquiere,Didier Vertommen,Verena Kriechbaumer,Kèvin Knoops,Carmen Lopez-Iglesias,Marc van Zandvoort,Jean-Charles Lambert,Julien Hanson,Christophe Desmet,Marc Thiry,Kyle J Lauersen,Marc Vidal,Pieter Van Vlierberghe,Franck Dequiedt,Kourosh Salehi-Ashtiani,Jean-Claude Twizere

Scientific reports 11:8619 PubMed33883639

2021

Novel approach for quantification of multiple immunofluorescent signals using histograms and 2D plot profiling of whole-section panoramic images.

Applications

Unspecified application

Species

Unspecified reactive species

Roko Duplancic,Darko Kero

Frontiers in physiology 10:1248 PubMed31611818

2019

Syndecans and Enzymes for Heparan Sulfate Biosynthesis and Modification Differentially Correlate With Presence of Inflammatory Infiltrate in Periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Roko Duplancic,Marija Roguljic,Ivan Puhar,Nika Vecek,Ruzica Dragun,Katarina Vukojevic,Mirna Saraga-Babic,Darko Kero

International journal of oncology 54:859-868 PubMed30664192

2019

A novel splice mutation induces exon skipping of the EXT1 gene in patients with hereditary multiple exostoses.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Guo,Mingrui Lin,Wei Yan,Wenxu Chen,Guolin Hong

Frontiers in physiology 9:732 PubMed29962964

2018

Syndecans and Enzymes Involved in Heparan Sulfate Biosynthesis and Degradation Are Differentially Expressed During Human Odontogenesis.

Applications

IHC-P

Species

Human

Darko Kero,Tanja Simic Bilandzija,Lidija Lasic Arapovic,Katarina Vukojevic,Mirna Saraga-Babic

PloS one 8:e72316 PubMed24009674

2013

Exome sequencing and functional analysis identifies a novel mutation in EXT1 gene that causes multiple osteochondromas.

Applications

IHC-P

Species

Human

Feng Zhang,Jinlong Liang,Xiong Guo,Yingang Zhang,Yan Wen,Qiang Li,Zengtie Zhang,Weijuan Ma,Lanlan Dai,Xuanzhu Liu,Ling Yang,Jun Wang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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