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AB194448

Anti-EYA1 antibody

5

(1 리뷰)

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(2 제품이 사용된 논문 )

Mouse Polyclonal EYA1 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EYA1 aa 100 to C-terminus.

대체 명칭 보기

Protein phosphatase EYA1, Eyes absent homolog 1, EYA1

2 이미지
Western blot - Anti-EYA1 antibody (AB194448)
  • WB

Supplier Data

Western blot - Anti-EYA1 antibody (AB194448)

The observed band size may not correspond to the predicted protein molecular weight as the immunogen (recombinant fragment) was used for the test lane.

All lanes:

Western blot - Anti-EYA1 antibody (ab194448) at 1/1000 dilution

All lanes:

EYA1 recombinant protein (immunizing peptide) at 0.2 µg

Predicted band size: 65 kDa

Observed band size: 34 kDa

false

Western blot - Anti-EYA1 antibody (AB194448)
  • WB

Supplier Data

Western blot - Anti-EYA1 antibody (AB194448)

All lanes:

Western blot - Anti-EYA1 antibody (ab194448) at 1/500 dilution

All lanes:

Jurkat cell lysate at 50 µg

Predicted band size: 65 kDa

false

주요 정보

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human EYA1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99502

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보완 제품

Primary Antibodies

AB252224

Anti-SIX1 antibody [1229]

BOND RX™ Validated|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-SIX1 antibody [1229] (AB252224)

  • 0
  • 0
Primary Antibodies

AB52635

Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

  • 5
  • 11
Primary Antibodies

AB79389

Anti-Pax2 antibody [EP3251]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (AB79389)

  • 4
  • 11
Primary Antibodies

AB109737

Anti-Dlx5 antibody [EPR4488]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dlx5 antibody [EPR4488] (AB109737)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purity
Whole antiserum
보관 버퍼
Constituents: 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The EYA1 protein known as Eyes Absent Homolog 1 acts as a transcriptional coactivator and tyrosine phosphatase. It has a molecular weight of approximately 65 kDa. EYA1 expresses in various tissues including the kidney eye and branchial arches during developmental stages. It plays essential roles in organogenesis by modulating gene expression via its interaction with other proteins.
Biological function summary

EYA1 contributes significantly to the formation of several organs during embryonic development. This protein is part of the EYA-SIX transcriptional complex and works closely with SIX family proteins to regulate the expression of multiple key developmental genes. Through its phosphatase activity EYA1 also affects phosphorylated protein substrates altering their function and stability.

Pathways

EYA1 plays an important role in the regulation of the WNT and SHH signaling pathways both of which are fundamental for embryonic development and cell differentiation. EYA1 interacts with related proteins like SIX1 aiding in the transcriptional regulation needed for cellular proliferation and differentiation. Interferences in these pathways can lead to developmental anomalies.

Mutations or dysregulation of EYA1 have links to conditions such as Branchio-Oto-Renal (BOR) syndrome and congenital cataracts. The association with BOR syndrome arises from EYA1's interaction with other related proteins including SIX1 that regulate the development of the ear kidney and certain facial structures. EYA1's role in ocular development also connects it to certain types of hereditary cataracts demonstrating its impact on normal physiological processes when mutated.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5 (By similarity). Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress (PubMed : 19234442). Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis (By similarity). Also has phosphatase activity with proteins phosphorylated on Ser and Thr residues (in vitro) (By similarity). Required for normal embryonic development of the craniofacial and trunk skeleton, kidneys and ears (By similarity). Together with SIX1, it plays an important role in hypaxial muscle development; in this it is functionally redundant with EYA2 (By similarity).
See full target information EYA1
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cellular and molecular medicine 25:10980-10989 PubMed34773364

2021

The deubiquitinase OTUB1 fosters papillary thyroid carcinoma growth through EYA1 stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Peiyi Xie,Qing Chao,Jiuang Mao,Yue Liu,Jiayu Fang,Jing Xie,Jing Zhen,Yongqi Ding,Bidong Fu,Yun Ke,Da Huang

Molecular medicine reports 22:1767-1774 PubMed32705258

2020

Silencing of BRF2 inhibits the growth and metastasis of lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Bian,Qiu Li,Qiaolian Li,Ruigen Pan
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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