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AB204954

Anti-Ezrin antibody [6F1C3]

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Mouse Monoclonal Ezrin antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EZR aa 250-500.

대체 명칭 보기

VIL2, EZR, Ezrin, Cytovillin, Villin-2, p81

5 이미지
Flow Cytometry - Anti-Ezrin antibody [6F1C3] (AB204954)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Ezrin antibody [6F1C3] (AB204954)

Flow cytometric analysis of A431 cells labeling Ezrin with ab204954 at 1/200 (green) or negative control (red)

Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [6F1C3] (AB204954)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [6F1C3] (AB204954)

Immunofluorescent analysis of Hela cells labeling Ezrin with ab204954 at 1/200 (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Western blot - Anti-Ezrin antibody [6F1C3] (AB204954)
  • WB

Supplier Data

Western blot - Anti-Ezrin antibody [6F1C3] (AB204954)

All lanes:

Western blot - Anti-Ezrin antibody [6F1C3] (ab204954) at 1/500 dilution

Lane 1:

Jurkat cell lysate

Lane 2:

A431 cell lysate

Lane 3:

MCF-7 cell lysate

Lane 4:

HeLa cell lysate

Predicted band size: 69 kDa

false

Western blot - Anti-Ezrin antibody [6F1C3] (AB204954)
  • WB

Supplier Data

Western blot - Anti-Ezrin antibody [6F1C3] (AB204954)

(Expected MW is 47.1 kDa)

All lanes:

Western blot - Anti-Ezrin antibody [6F1C3] (ab204954) at 1/500 dilution

All lanes:

Human Ezrin (aa: 292-464) recombinant protein.

Predicted band size: 69 kDa

false

Western blot - Anti-Ezrin antibody [6F1C3] (AB204954)
  • WB

Supplier Data

Western blot - Anti-Ezrin antibody [6F1C3] (AB204954)

All lanes:

Western blot - Anti-Ezrin antibody [6F1C3] (ab204954) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

Ezrin (AA: 292-464)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 69 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

6F1C3

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human EZR aa 250-500. The exact immunogen used to generate this antibody is proprietary information.

P15311

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대체품

Primary Antibodies

AB4069

Anti-Ezrin antibody [3C12]

BOND RX™ Validated|KO Validated

Flow Cytometry (Intracellular) - Anti-Ezrin antibody [3C12] (AB4069)

  • 3
  • 8
Primary Antibodies

AB198522

HRP Anti-Ezrin antibody [EP886Y]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Ezrin antibody [EP886Y] (AB198522)

  • 0
  • 0
Primary Antibodies

AB133297

Anti-Ezrin antibody [EPR803]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR803] (AB133297)

  • 0
  • 0
Primary Antibodies

AB40839

Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker

20ul selling size|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (AB40839)

  • 5
  • 5
Primary Antibodies

AB198520

Alexa Fluor® 488 Anti-Ezrin antibody [EP886Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Ezrin antibody [EP886Y] (AB198520)

  • 0
  • 0
Primary Antibodies

AB75840

Anti-Ezrin antibody [EP924Y]

20ul selling size|RabMAb|KO Validated|Recombinant

Western blot - Anti-Ezrin antibody [EP924Y] (AB75840)

  • 0
  • 0
Primary Antibodies

AB239832

Anti-Ezrin antibody [EP886Y] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (AB239832)

  • 0
  • 0

보완 제품

Assay Kits

AB278116

Human Ezrin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Ezrin ELISA Kit (AB278116)

  • 0
  • 0
Primary Antibodies

AB92516

Anti-Calreticulin antibody [EPR3924] - ER Marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calreticulin antibody [EPR3924] - ER Marker (AB92516)

  • 4
  • 6
Primary Antibodies

AB125068

Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker

20ul selling size|RabMAb|Recombinant|Lab Essentials|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker (AB125068)

  • 4
  • 4
Primary Antibodies

AB179484

Anti-alpha Tubulin (acetyl K40) antibody [EPR16772]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin (acetyl K40) antibody [EPR16772] (AB179484)

  • 5
  • 4
Primary Antibodies

AB130751

Anti-Villin antibody [SP145]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry (Intracellular) - Anti-Villin antibody [SP145] (AB130751)

  • 5
  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purified from tissue culture supernatant.
보관 버퍼
Preservative: 0.05% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ezrin also known as cytovillin or villin-2 is a protein belonging to the ezrin-radixin-moesin (ERM) family. It is approximately 81 kDa in molecular weight. Ezrin plays a critical role in linking the actin cytoskeleton to the plasma membrane. This interaction occurs through its ability to bind to membrane proteins and phospholipids facilitating structural support and signal transduction. Ezrin is widely expressed in epithelial tissues but it also occurs in other cell types such as lymphocytes and osteoclasts.
Biological function summary

Ezrin functions as a membrane-microfilament linker and is essential for maintaining cell shape adhesion and motility. It is an integral component of many cellular structures including microvilli and ruffles. Ezrin’s activity often involves forming complexes with other proteins such as EBP50 and F-actin which are fundamental for its role in cellular morphogenesis. These interactions allow ezrin to regulate membrane surface structure and cell surface organization.

Pathways

Ezrin participates in various signaling pathways that are essential for cell migration and invasion. One key pathway is the Rho family of GTPases which regulates actin cytoskeleton dynamics. Ezrin interacts with proteins like Rho-associated kinase (ROCK) that influence cell contraction and motility. Additionally ezrin is involved in the MAPK/ERK pathway which influences cell growth and survival by interacting with other signaling molecules.

Ezrin shows significant relevance to cancer progression and metastasis particularly in conditions like osteosarcoma. Ezrin overexpression correlates with increased metastatic potential and poor clinical outcomes. It also has implications in allergic diseases due to its role in immune cell function. In these contexts ezrin interacts with SRC family kinases which are important for transmitting signals from cell-surface receptors to downstream pathways influencing disease development and progression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
See full target information EZR
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Structure (London, England : 1993) 26:446-458.e8 PubMed29478821

2018

Disease Variants of FGFR3 Reveal Molecular Basis for the Recognition and Additional Roles for Cdc37 in Hsp90 Chaperone System.

Applications

Unspecified application

Species

Unspecified reactive species

Tom D Bunney,Alison J Inglis,Domenico Sanfelice,Brendan Farrell,Christopher J Kerr,Gary S Thompson,Glenn R Masson,Nethaji Thiyagarajan,Dmitri I Svergun,Roger L Williams,Alexander L Breeze,Matilda Katan
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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