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AB235927

Anti-Ezrin antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal Ezrin antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EZR aa 1-300.

대체 명칭 보기

VIL2, EZR, Ezrin, Cytovillin, Villin-2, p81

3 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody (AB235927)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody (AB235927)

4% paraformaldehyde-fixed. 0.2% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ezrin (green) using ab235927 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody (AB235927)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody (AB235927)

Paraffin-embedded human brain tissue stained for Ezrin using ab235927 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody (AB235927)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody (AB235927)

Paraffin-embedded human tonsil tissue stained for Ezrin using ab235927 at 1/300 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EZR aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P15311

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대체품

Primary Antibodies

AB40839

Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker

20ul selling size|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (AB40839)

  • 5
  • 5
Primary Antibodies

AB4069

Anti-Ezrin antibody [3C12]

BOND RX™ Validated|KO Validated

Flow Cytometry (Intracellular) - Anti-Ezrin antibody [3C12] (AB4069)

  • 3
  • 8
Primary Antibodies

AB198522

HRP Anti-Ezrin antibody [EP886Y]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Ezrin antibody [EP886Y] (AB198522)

  • 0
  • 0
Primary Antibodies

AB133297

Anti-Ezrin antibody [EPR803]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EPR803] (AB133297)

  • 0
  • 0
Primary Antibodies

AB198520

Alexa Fluor® 488 Anti-Ezrin antibody [EP886Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Ezrin antibody [EP886Y] (AB198520)

  • 0
  • 0
Primary Antibodies

AB75840

Anti-Ezrin antibody [EP924Y]

20ul selling size|RabMAb|KO Validated|Recombinant

Western blot - Anti-Ezrin antibody [EP924Y] (AB75840)

  • 0
  • 0
Primary Antibodies

AB239832

Anti-Ezrin antibody [EP886Y] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (AB239832)

  • 0
  • 0

보완 제품

Primary Antibodies

AB52490

Anti-Moesin antibody [EP1863Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Moesin antibody [EP1863Y] (AB52490)

  • 5
  • 5
Primary Antibodies

AB76247

Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (AB76247)

  • 5
  • 2
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB52495

Anti-Radixin/RDX antibody [EP1862Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Radixin/RDX antibody [EP1862Y] (AB52495)

  • 5
  • 4
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%.
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ezrin also known as cytovillin or villin-2 is a protein belonging to the ezrin-radixin-moesin (ERM) family. It is approximately 81 kDa in molecular weight. Ezrin plays a critical role in linking the actin cytoskeleton to the plasma membrane. This interaction occurs through its ability to bind to membrane proteins and phospholipids facilitating structural support and signal transduction. Ezrin is widely expressed in epithelial tissues but it also occurs in other cell types such as lymphocytes and osteoclasts.
Biological function summary

Ezrin functions as a membrane-microfilament linker and is essential for maintaining cell shape adhesion and motility. It is an integral component of many cellular structures including microvilli and ruffles. Ezrin's activity often involves forming complexes with other proteins such as EBP50 and F-actin which are fundamental for its role in cellular morphogenesis. These interactions allow ezrin to regulate membrane surface structure and cell surface organization.

Pathways

Ezrin participates in various signaling pathways that are essential for cell migration and invasion. One key pathway is the Rho family of GTPases which regulates actin cytoskeleton dynamics. Ezrin interacts with proteins like Rho-associated kinase (ROCK) that influence cell contraction and motility. Additionally ezrin is involved in the MAPK/ERK pathway which influences cell growth and survival by interacting with other signaling molecules.

Ezrin shows significant relevance to cancer progression and metastasis particularly in conditions like osteosarcoma. Ezrin overexpression correlates with increased metastatic potential and poor clinical outcomes. It also has implications in allergic diseases due to its role in immune cell function. In these contexts ezrin interacts with SRC family kinases which are important for transmitting signals from cell-surface receptors to downstream pathways influencing disease development and progression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
See full target information EZR
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in immunology 15:1477267 PubMed39399493

2024

The pathological significance and potential mechanism of ACLY in cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Sun,Xiaofang Zhao,Senyan Wang,Qi Liu,Wenjuan Wei,Jing Xu,Hongyang Wang,Wen Yang

Investigative ophthalmology & visual science 65:2 PubMed39087934

2024

Functional Characterization of ABCA4 Missense Variants Aids Variant Interpretation and Phenotype Prediction in Patients With ABCA4-Retinal Dystrophies.

Applications

Unspecified application

Species

Unspecified reactive species

Sigrid Aslaksen,Ingvild Aukrust,Laurie Molday,Josephine Prener Holtan,Ragnhild Wivestad Jansson,Siren Berland,Eyvind Rødahl,Cecilie Bredrup,Ragnheiður Bragadóttir,Eirik Bratland,Robert S Molday,Per Morten Knappskog
제품이 사용된 논문 모두 보기
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Product promise

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