Anti-F4/80 antibody - C-terminal

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody - C-terminal (AB186073)

Chicken anti mouse F4/80 (ab186073, polyclonal) staining of mouse RAW cells by immunofluorescence, using a FITC-conjugated anti chicken antibody as a secondary antibody.

• WB

Supplier Data

Western blot - Anti-F4/80 antibody - C-terminal (AB186073)

All lanes:

Western blot - Anti-F4/80 antibody - C-terminal (ab186073) at 1 µg/mL

All lanes:

Mouse spleen lysate

Predicted band size: 97 kDa

true

Exposure time: 5s

• ICC

CiteAb

Immunocytochemistry - Anti-F4/80 antibody - C-terminal (AB186073)

Immunocytochemistry using Anti-F4/80 antibody - C-terminal, ab186073. Publication image from Amini-Nik, S. et al., 2018, Exp Mol Med, 30026607. Legend direct from paper.

Myeloid cells have an essential role in the induction of fibrosis post-thermal injury. a Mice treated with liposomal clodronate compared with control treated group. Trichrome staining shows less fibrosis around portal venules post-thermal injury when myeloid cells are depleted; n = 9, scale bar 50 µm. b Immunohistochemistry shows a highly effective ablation of F4/80+ cells in liposomal clodronate-treated group in comparison to the control group; n = 9, scale bar 50 µm. c There is a global ablation of F4/80+ cells in the liver of mice treated with liposomal clodronate, scale bar 100 µm. d In contrast to earlier findings, without F4/80+ cells, there is an increase in desmin+ cells post-thermal injury; n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

• WB

CiteAb

Western blot - Anti-F4/80 antibody - C-terminal (AB186073)

F4/80 western blot using anti-F4/80 antibody - C-terminal ab186073. Publication image and figure legend from Amini-Nik, S., Sadri, A. R., et al., 2018, Exp Mol Med, PubMed 30026607.

ab186073 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab186073 please see the product overview.

Myeloid cells have an essential role in the induction of fibrosis post-thermal injury. a Mice treated with liposomal clodronate compared with control treated group. Trichrome staining shows less fibrosis around portal venules post-thermal injury when myeloid cells are depleted; n = 9, scale bar 50 μm. b Immunohistochemistry shows a highly effective ablation of F4/80+ cells in liposomal clodronate-treated group in comparison to the control group; n = 9, scale bar 50 μm. c There is a global ablation of F4/80+ cells in the liver of mice treated with liposomal clodronate, scale bar 100 μm. d In contrast to earlier findings, without F4/80+ cells, there is an increase in desmin+ cells post-thermal injury; n = 3. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

false