AB54615

Anti-FAAH1 antibody [4H8]

Name: Anti-FAAH1 antibody [4H8]
Brand: Abcam Limited
SKU: AB54615
Rating: 5 (6 reviews)

(6 리뷰들)

(37 제품이 사용된 논문 )

Mouse Monoclonal FAAH1 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 37 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAAH aa 450 to C-terminus.

3 이미지

Unknown

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)

FAAH antibody (ab54615) at 1ug/lane + A-431 cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-FAAH1 antibody [4H8] (ab54615)

Predicted band size: 63 kDa

false

Unknown

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-FAAH1 antibody [4H8] (ab54615)

All lanes:

Rat Brain

Predicted band size: 63 kDa

false

Unknown

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-FAAH1 antibody [4H8] (ab54615)

All lanes:

NIH/3T3

Predicted band size: 63 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

4H8

Isotype

IgG2a

Light chain 타입

kappa

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB, IP

applications

Immunogen

Recombinant Fragment Protein within Human FAAH aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00519

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "See Abreview.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "See Abreview.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "See Abreview.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

제품 세부 정보

This product was changed from ascites to tissue culture supernatant on 17/04/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

style

left-column

특성 및 보관 정보

제형

Liquid

Purity

Tissue culture supernatant

보관 버퍼

pH: 7.4 Constituents: 91% Water, 8% Sodium chloride, 0.6% Disodium hydrogenorthophosphate, 0.2% Potassium chloride, 0.2% Potassium phosphate monobasic

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Fatty acid amide hydrolase 1 (FAAH1) often referred to simply as FAAH is an enzyme that plays a critical role in the degradation of fatty acid amides such as anandamide in the body. This enzyme weighing approximately 63 kDa is a part of the amidase signature family and is characterized by its amidase hydrolase activity. FAAH1 is predominantly expressed in the nervous system liver and gastrointestinal tract where it hydrolyses amide bonds facilitating the termination of signaling pathways initiated by neurotransmitter-like fatty acid amides.

Biological function summary

FAAH1 is essential for maintaining homeostasis by modulating levels of bioactive lipids. It exerts control over endocannabinoid signaling by breaking down anandamide a lipid derivative functioning as a neurotransmitter. FAAH1 does not act as part of a larger protein complex. Instead it operates alone to regulate the duration and intensity of signaling rather than relying on direct interactions within a complex. The enzyme's function impacts the neurochemical milieu in tissues where it is actively expressed thereby affecting mood pain sensation and more.

Pathways

FAAH1 has a significant role in the endocannabinoid signaling pathway and fatty acid metabolism. Within these pathways it modulates signaling by hydrolysing bioactive amides like anandamide influencing overall lipid signaling. FAAH1 activities intersect with several other proteins such as cannabinoid receptors which bind the substrates that FAAH1 eventually degrades. These interactions are vital in controlling signal transduction processes that regulate physiological responses including stress and analgesia.

FAAH1 has been implicated in conditions like chronic pain and anxiety disorders. Altered FAAH1 function or expression can lead to dysregulation of endocannabinoid signaling contributing to the pathophysiology of these conditions. The enzyme's modulation of anandamide levels connects it to proteins such as CB1 and CB2 cannabinoid receptors which mediate the effects of endocannabinoids in neurological and psychiatric disorders. Research into FAAH1 inhibitors as therapeutic agents highlights its importance in potential treatments for managing pain and anxiety.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules (PubMed : 17015445, PubMed : 19926788, PubMed : 9122178). Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates (PubMed : 17015445, PubMed : 9122178). It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol) (PubMed : 21049984). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids (By similarity).

See full target information FAAH

대체 명칭 보기

FAAH1, FAAH, Fatty-acid amide hydrolase 1, Anandamide amidohydrolase 1, Fatty acid ester hydrolase, Oleamide hydrolase 1

style

left-column

제품이 사용된 논문 (37)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cells 14: PubMed40643519

2025

Short-Term Incubation of H9c2 Cardiomyocytes with Cannabigerol Attenuates Diacylglycerol Accumulation in Lipid Overload Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Sylwia Dziemitko,Adrian Chabowski,Ewa Harasim-Symbor

Journal of neurochemistry 169:e16256 PubMed39556462

2024

Alterations of endocannabinoid signaling and microglia reactivity in the retinas of AD-like mice precede the onset of hippocampal β-amyloid plaques.

Applications

Unspecified application

Species

Unspecified reactive species

Annamaria Tisi,Lucia Scipioni,Giulia Carozza,Lucia Di Re,Giacomo Cimino,Camilla Di Meo,Sakthimala Palaniappan,Francesco Della Valle,Federico Fanti,Giacomo Giacovazzo,Dario Compagnone,Rita Maccarone,Sergio Oddi,Mauro Maccarrone

Science advances 10:eadp2622 PubMed39441940

2024

Single-cell chemoproteomics identifies metastatic activity signatures in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kavya Smitha Pillai,Olivia Laxton,Gang Li,Jing Lin,Olga Karginova,Rita Nanda,Olufunmilayo I Olopade,Savaş Tay,Raymond E Moellering

PloS one 19:e0305868 PubMed38913661

2024

Cannabinoid receptor 1 positive allosteric modulator ZCZ011 shows differential effects on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Barkha J Yadav-Samudrala,Hailey Dodson,Shreya Ramineni,Elizabeth Kim,Justin L Poklis,Dai Lu,Bogna M Ignatowska-Jankowska,Aron H Lichtman,Sylvia Fitting

Pharmaceuticals (Basel, Switzerland) 17: PubMed38543105

2024

Evaluating Fatty Acid Amide Hydrolase as a Suitable Target for Sleep Promotion in a Transgenic TauP301S Mouse Model of Neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Shenée C Martin,Kathryn K Joyce,Kathryn M Harper,Samuel J Harp,Todd J Cohen,Sheryl S Moy,Graham H Diering

Oncotarget 14:910-918 PubMed37921652

2023

FAAH inhibition ameliorates breast cancer in a murine model.

Applications

Unspecified application

Species

Unspecified reactive species

Mallika Tripathy,Amy Bui,Jared Henderson,Jeffrey Sun,Christian Rutan Woods,Soumya Somani,Thao Doan,Anto Sam Crosslee Louis Sam Titus,Chandra Mohan

Brain research 1822:148638 PubMed37858856

2023

Effects of acute Δ-tetrahydrocannabinol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Barkha J Yadav-Samudrala,Benjamin L Gorman,Hailey Dodson,Shreya Ramineni,E Diane Wallace,Michelle R Peace,Justin L Poklis,Wei Jiang,Sylvia Fitting

International journal of molecular sciences 24: PubMed37686443

2023

Renal Endocannabinoid Dysregulation in Obesity-Induced Chronic Kidney Disease in Humans.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Permyakova,Ariel Rothner,Sarah Knapp,Alina Nemirovski,Danny Ben-Zvi,Joseph Tam

STAR protocols 3:101778 PubMed36313539

2022

A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengyuan Pang,Li Ye

Cell death and differentiation 30:168-183 PubMed36104448

2022

FAAH served a key membrane-anchoring and stabilizing role for NLRP3 protein independently of the endocannabinoid system.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Zhu,Hao Zhang,Huawei Mao,Suqin Zhong,Yubing Huang,Sirong Chen,Kai Yan,Zhibin Zhao,Xiaohan Hao,Yue Zhang,Han Yao,Xiaowan Huang,Meimei Wang,Wenbin Zhang,Juan Li,Guangxun Meng,Xiaohua Qin,Zhiming Ye,Jiani Shen,Yang Song,Youcui Xu,Zhenyu Yang,Liansheng Wang,Yunjiao Zhang,Longping Wen

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAAH1 antibody [4H8]

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)

Western blot - Anti-FAAH1 antibody [4H8] (AB54615)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purity

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (37)

Product promise