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AB240164

Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal FABP-1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

대체 명칭 보기

KYAT4, GOT2, mAspAT, Fatty acid-binding protein, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, Transaminase A, FABP-1, FABPpm

7 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)

Immunohistochemistry of paraffin-embedded Human urinary bladder carcinoma tissue labeling FABP-1 using ab171739 (unpurified) at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling FABP-1 with purified ab171739 at 1/4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)

Immunohistochemistry of paraffin-embedded Human liver tissue labeling FABP-1 using ab171739 (unpurified) at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739).

Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
  • IP

Supplier Data

Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)

Western blot analysis on immunoprecipitation pellet from Human fetal liver lysate (lane 1) labeling FABP-1 using ab171739 (unpurified) at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. Lane 2 : 1X PBS (negative control).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739).

All lanes:

Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] (<a href='/ko/products/primary-antibodies/fabp-1-antibody-epr12354b-ab171739'>ab171739</a>)

Predicted band size: 48 kDa

false

Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
  • IP

Unknown

Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)

ab171739 (purified ) at 1/50 dilution (2 µg) immunoprecipitating FABP-1 in Human fetal liver lysate.
Lane 1 (input) : Human fetal liver lysate 10 µg
Lane 2 (+) : ab171739 & Human fetal liver lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab171739 in Human fetal liver lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)

All lanes:

Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] (<a href='/ko/products/primary-antibodies/fabp-1-antibody-epr12354b-ab171739'>ab171739</a>)

Predicted band size: 48 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling FABP-1 with purified ab171739 at 1/4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling FABP-1 with purified ab171739 at 1/4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)

관련 conjugated 항체와 다양한 조성의 항체 (8)

  • Unconjugated

    Anti-FABP-1 antibody [EPR12354(B)]

  • 660 APC

    APC Anti-FABP-1 antibody [EPR12354(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-FABP-1 antibody [EPR12354(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-FABP-1 antibody [EPR12354(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-FABP-1 antibody [EPR12354(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-FABP-1 antibody [EPR12354(B)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-FABP-1 antibody [EPR12354(B)]

  • 578 PE

    PE Anti-FABP-1 antibody [EPR12354(B)]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12354(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

제품 세부 정보

ab240164 is the carrier-free version of ab171739.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.
See full target information GOT2

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 43:494-500 PubMed37147750

2023

Electroacupuncture alleviates water avoidance stress-induced irritable bowel syndrome in mice by improving intestinal barrier functions and suppressing the expression of inflammatory cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Sun Mengzhu,Zhang Yujie,Song Yafang,Guo Jing,Wang Yuhang,Xin Chen,G U Dongmei,Sun Jianhua,Pei Lixia

Biomedicine & pharmacotherapy = Biomedecine & phar 118:109251 PubMed31351426

2019

PPAR signaling pathway in the first trimester placenta from in vitro fertilization and embryo transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhao,Xiuli Zheng,Jingfang Liu,Rong Zheng,Rui Yang,Ying Wang,Lifang Sun
제품이 사용된 논문 모두 보기

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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