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AB72658

Anti-Factor B antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal CFAB antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CFB aa 250-300.
1 이미지
Western blot - Anti-Factor B antibody (AB72658)
  • WB

Unknown

Western blot - Anti-Factor B antibody (AB72658)

All lanes:

Western blot - Anti-Factor B antibody (ab72658) at 1/200 dilution

Lane 1:

Extracts from K562 cells, treated with etoposide (25uM, 1hour) at 5 µg

Lane 2:

Extracts from K562 cells, treated with etoposide (25uM, 1hour) with Cl-peptide at 5 µg at 5 µg

Predicted band size: 85 kDa

Observed band size: 40 kDa,54 kDa,75 kDa,86 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human CFB aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

P00751

Specificity

ab72658 detects a fragment from activated Factor B; the Bb fragment, resulting from cleavage adjacent to Lys260.

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대체품

Primary Antibodies

AB133765

Anti-Complement factor B antibody [EPR9288(B)]

RabMAb|Recombinant|Trial Size

Western blot - Anti-Complement factor B antibody [EPR9288(B)] (AB133765)

  • 0
  • 0
Primary Antibodies

AB110651

Anti-Factor B antibody [KT21]

Sandwich ELISA - Anti-Factor B antibody [KT21] (AB110651)

  • 0
  • 0
Primary Antibodies

AB17927

Anti-Factor B antibody [6G11]

  • 0
  • 0
Primary Antibodies

AB17932

Anti-Factor B antibody [9B8]

  • 0
  • 0
Primary Antibodies

AB192577

Anti-Factor B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor B antibody (AB192577)

  • 0
  • 0
Primary Antibodies

AB231072

Anti-Factor B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor B antibody (AB231072)

  • 0
  • 0
Primary Antibodies

AB106687

HRP Anti-Factor B antibody [KT24]

Sandwich ELISA - HRP Anti-Factor B antibody [KT24] (AB106687)

  • 0
  • 0

보완 제품

Primary Antibodies

AB200999

Anti-C3 antibody [EPR19394]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-C3 antibody [EPR19394] (AB200999)

  • 4
  • 9
Primary Antibodies

AB192890

Anti-LC3B antibody [EPR18709] - Autophagosome Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB192890)

  • 5
  • 19
Primary Antibodies

AB32047

Anti-AMPK alpha 1 antibody [Y365]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-AMPK alpha 1 antibody [Y365] (AB32047)

  • 5
  • 6
Primary Antibodies

AB263899

Anti-NLRP3 antibody [EPR23094-1]

KO Validated|RabMAb|Recombinant|20ul selling size

Western blot - Anti-NLRP3 antibody [EPR23094-1] (AB263899)

  • 4
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Factor B also known as B-factor is a protein that plays an important role in the immune system specifically in the complement pathway. It is sometimes referred to as factor B complement and its gene identifier is often referenced as KY21 NNL or factor B 1001. Factor B has a molecular mass of approximately 93 kDa. It is mainly expressed in the liver but can also be found in blood plasma in its inactive form as part of the innate immune response.
Biological function summary

Factor B is involved in the alternative complement pathway which serves as a part of the body's defense mechanism. It participates in the formation of the C3 convertase together with factor D and properdin initiating a cascade that can lead to the opsonization and elimination of pathogens. Factor B undergoes cleavage by factor D which generates the Ba and Bb fragments with Bb functioning as a serine protease within the C3 convertase complex.

Pathways

Factor B contributes to the complement system interacting notably in the alternative and lectin pathways. In these pathways Factor B associates with proteins like complement component C3. The alternative pathway is activated on microbial surfaces independent of antibodies. The involvement of Factor B in these pathways illustrates its role in pathogen recognition and further highlights its cooperation with complement proteins such as C3 and properdin.

Factor B has been associated with atypical hemolytic uremic syndrome (aHUS) a rare and serious disease affecting kidney function. Factor B involvement in aHUS links it to abnormal complement activation leading to a pathological condition. Additionally Factor B connects to age-related macular degeneration (AMD) a disorder where complement pathway genetic variations including those involving Factor B have shown associations. Understanding interactions with other complement components such as factor H helps to elucidate its role in these diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). The alternative complement pathway acts as an amplification loop that enhances other complement pathways (classical, lectin and GZMK) by promoting formation of additional C3 and C5 convertases (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). CFB is cleaved and activated by CFD to generate Ba and Bb chains; Bb chain constituting the catalytic component of the C3 and C5 convertases (PubMed : 6769474, PubMed : 9748277).. Complement factor B Bb. Serine protease component of the complement C3 and C5 convertase complexes of the alternative complement pathway (PubMed : 30643019, PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543). Following cleavage and activation by factor D (CFD), forms the C3 convertase together with complement C3b (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). As part of the C3 convertase, cleaves and activates C3 into C3a anaphylatoxin and C3b opsonin, the next components of the complement pathways (PubMed : 3638964, PubMed : 624565, PubMed : 6554279, PubMed : 6919543, PubMed : 9748277). When an additional complement C3b molecule binds to the C3 convertase, forms the C5 convertase, which cleaves and activates C5 into C5a anaphylatoxin and C5b component of the membrane attack complex (PubMed : 30643019, PubMed : 624565, PubMed : 6554279).. Complement factor B Ba. Involved in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes.
See full target information CFB

대체 명칭 보기

BF, BFD, CFB, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in immunology 11:154 PubMed32117292

2020

A Systematic Investigation on Complement Pathway Activation in Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Shahna Shahulhameed,Sushma Vishwakarma,Jay Chhablani,Mudit Tyagi,Rajeev R Pappuru,Saumya Jakati,Subhabrata Chakrabarti,Inderjeet Kaur

Nucleic acids research 44:10974-10985 PubMed27536005

2016

A deafness-associated tRNAAsp mutation alters the m1G37 modification, aminoacylation and stability of tRNAAsp and mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wang,Yanyan Peng,Jing Zheng,Binjiao Zheng,Xiaofen Jin,Hao Liu,Yong Wang,Xiaowen Tang,Taosheng Huang,Pingping Jiang,Min-Xin Guan

Transplantation 96:791-9 PubMed23958924

2013

Activation of the lectin pathway of complement in pig-to-human xenotransplantation models.

Applications

Unspecified application

Species

Unspecified reactive species

Anjan K Bongoni,David Kiermeir,Hansjörg Jenni,Annegret Wünsch,Andrea Bähr,David Ayares,Jörg D Seebach,Eckhard Wolf,Nikolai Klymiuk,Mihai A Constantinescu,Esther Vögelin,Robert Rieben
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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