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AB244574

Anti-Factor H antibody [OX-24] - BSA and Azide free

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Mouse Monoclonal Factor H antibody. Carrier free. Suitable for WB, ELISA and reacts with Human, Purified native protein, Recombinant full length protein - Human samples. Cited in 1 publication.

대체 명칭 보기

HF, HF1, HF2, CFH, Complement factor H, H factor 1

1 이미지
Western blot - Anti-Factor H antibody [OX-24] - BSA and Azide free (AB244574)
  • WB

Lab

Western blot - Anti-Factor H antibody [OX-24] - BSA and Azide free (AB244574)

This image was produced using the same antibody clone but in a different formulation (ab118820).

This blot was produced using a 4-12% Bis-tris under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab118820 overnight at 4°C at a 1μg/ml concentration. Antibody binding was detected using Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Factor H antibody [OX-24] (<a href='/ko/products/primary-antibodies/factor-h-antibody-ox-24-ab118820'>ab118820</a>) at 1 µg/mL

Lane 1:

Human serum diluted 1/100 at 10 µL

Lane 2:

Human plasma diluted 1/100 at 10 µL

Lane 3:

Purified Factor H protein at 0.5 µg

Lane 4:

Purified Factor H protein at 0.1 µg

Predicted band size: 139 kDa

Observed band size: 170 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OX-24

Isotype

IgG1

Light chain 타입

kappa

Carrier free

Yes

Reacts with

Human

Applications

ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB121055

Anti-Factor H antibody [C18/3]

  • 0
  • 0
Primary Antibodies

AB124769

Anti-Factor H antibody [EPR6226]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Factor H antibody [EPR6226] (AB124769)

  • 0
  • 0
Primary Antibodies

AB133536

Anti-Factor H antibody [EPR6225]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Factor H antibody [EPR6225] (AB133536)

  • 0
  • 0
Primary Antibodies

AB248539

Anti-Factor H antibody [EPR6225] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-Factor H antibody [EPR6225] - BSA and Azide free (AB248539)

  • 0
  • 0
Primary Antibodies

AB247995

Anti-Factor H antibody [EPR6226] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-Factor H antibody [EPR6226] - BSA and Azide free (AB247995)

  • 0
  • 0

보완 제품

Assay Kits

AB252359

Human Complement factor H ELISA Kit

Recombinant|SimpleStep

ELISA - Human Complement factor H ELISA Kit (AB252359)

  • 0
  • 0
Primary Antibodies

AB200999

Anti-C3 antibody [EPR19394]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-C3 antibody [EPR19394] (AB200999)

  • 4
  • 9
Primary Antibodies

AB66768

Anti-C5b-9 + C5b-8 antibody [aE11]

Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (AB66768)

  • 5
  • 1
Proteins & Peptides

AB131757

Recombinant Human Factor H protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Factor H protein (GST tag N-Terminus) (AB131757)

  • 0
  • 0
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Purified native protein": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab244574 is the carrier-free version of ab118820.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Factor H also known as complement factor H is an important regulatory protein in the complement system. It has a molecular mass of approximately 155 kDa. This protein is mainly expressed in the liver but it can also be found in low levels in other tissues. Factor H serves as a control element for complement activation particularly affecting the alternative pathway. It binds to C3b a central component of the complement system and accelerates the decay of C3 convertase as well as promotes the proteolytic inactivation of C3b by factor I.
Biological function summary

Factor H limits the activity of the complement system to prevent damage to host tissues. The protein exists in the plasma in a soluble form. It functions by recognizing host cell surfaces via specific markers avoiding inappropriate activation. Factor H belongs to a group of proteins which include other regulators of complement activation. These proteins maintain the balance between effective immune defense and protection of host tissue from excessive immune responses.

Pathways

Factor H is a part of the alternative complement pathway. This pathway is important for innate immune response involving proteins like factor P (properdin) which stabilizes C3 convertase. Factor H modulates these interactions to prevent unwarranted complement activity on host cells. Another related pathway is the classic complement pathway although factor H's involvement here is less direct since it primarily regulates the alternative pathway.

Factor H associations include atypical hemolytic uremic syndrome and age-related macular degeneration. Factor H deficiency or dysfunction can lead to uncontrolled complement activation resulting in kidney damage in atypical hemolytic uremic syndrome where it is also related to factor I. Additionally in age-related macular degeneration variants in the factor H gene are linked to increased susceptibility further highlighting the protein's importance in regulating immune responses.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Glycoprotein that plays an essential role in maintaining a well-balanced immune response by modulating complement activation. Acts as a soluble inhibitor of complement, where its binding to self markers such as glycan structures prevents complement activation and amplification on cell surfaces (PubMed : 21285368, PubMed : 21317894, PubMed : 25402769). Accelerates the decay of the complement alternative pathway (AP) C3 convertase C3bBb, thus preventing local formation of more C3b, the central player of the complement amplification loop (PubMed : 19503104, PubMed : 21317894, PubMed : 26700768). As a cofactor of the serine protease factor I, CFH also regulates proteolytic degradation of already-deposited C3b (PubMed : 18252712, PubMed : 23332154, PubMed : 28671664). In addition, mediates several cellular responses through interaction with specific receptors. For example, interacts with CR3/ITGAM receptor and thereby mediates the adhesion of human neutrophils to different pathogens. In turn, these pathogens are phagocytosed and destroyed (PubMed : 20008295, PubMed : 9558116).. (Microbial infection) In the mosquito midgut, binds to the surface of parasite P.falciparum gametocytes and protects the parasite from alternative complement pathway-mediated elimination.
See full target information CFH
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

European journal of immunology 15:168-73 PubMed2578966

1985

Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

G R Woollett,A N Barclay,M Puklavec,A F Williams
제품이 사용된 논문 모두 보기
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Product promise

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