AB232898

Anti-FADS2 antibody

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(10 제품이 사용된 논문 )

Rabbit Polyclonal FADS2 antibody. Suitable for WB, IHC-P and reacts with Pig, Recombinant fragment - Human, Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FADS2 aa 1-150.

대체 명칭 보기

Acyl-CoA 6-desaturase, Delta(6) fatty acid desaturase, Fatty acid desaturase 2, D6D, Delta(6) desaturase, Delta-6 desaturase, FADS2

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (AB232898)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (AB232898)

Formalin-fixed, paraffin-embedded human cerebrum tissue stained for FADS2 using ab232898 at 30 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (AB232898)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (AB232898)

Formalin-fixed, paraffin-embedded human liver tissue stained for FADS2 using ab232898 at 30 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (AB232898)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (AB232898)

Formalin-fixed, paraffin-embedded human kidney tissue stained for FADS2 using ab232898 at 30 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (AB232898)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (AB232898)

Formalin-fixed, paraffin-embedded human breast cancer tissue stained for FADS2 using ab232898 at 30 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (AB232898)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADS2 antibody (AB232898)

Formalin-fixed, paraffin-embedded human glioma tissue stained for FADS2 using ab232898 at 30 μg/ml in immunohistochemical analysis.

Secondary used : HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 μg/ml. DAB staining.

Western blot - Anti-FADS2 antibody (AB232898)

WB

Supplier Data

Western blot - Anti-FADS2 antibody (AB232898)

19kDa is oberserved because this is image is produced with part of the FADS2 protein (aa 1-130) and not from full length.

All lanes:

Western blot - Anti-FADS2 antibody (ab232898) at 1 µg/mL

All lanes:

Recombinant human FADS2 protein

Secondary

All lanes:

HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution

Predicted band size: 52 kDa

Observed band size: 19 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB, IHC-P

Immunogen

Recombinant Fragment Protein within Human FADS2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>Pig species is recommended based on WB results, we do not guarantee IHC-P for pig.</p>

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

보관 버퍼

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FADS2 also known as Fatty Acid Desaturase 2 or FABRED27 functions as an enzyme involved in creating double bonds in specific fatty acids. It holds a molecular weight of approximately 52 kDa. Researchers find this enzyme prominently expressed in liver tissue and it plays an essential role in the process of lipid metabolism.

Biological function summary

FADS2 contributes to the metabolism of polyunsaturated fatty acids by introducing double bonds specifically within the fatty acid synthetic pathway. It does not function as part of a larger complex but operates independently. Through its enzymatic action it influences the production of essential fatty acids such as arachidonic acid and eicosapentaenoic acid which are important for maintaining normal cellular function.

Pathways

FADS2 plays an integral part within both the fatty acid biosynthesis and fatty acid metabolism pathways. It closely relates with other enzymes such as acyl-CoA synthetase and elongation enzymes which work together to metabolize various fats within the body. These pathways help maintain cellular homeostasis by regulating levels of essential long-chain polyunsaturated fatty acids.

Alterations in FADS2 activity have connections to metabolic disorders like obesity and cardiovascular disease. Aberrant expression of FADS2 can influence lipid profiles in these conditions potentially exacerbating disease outcomes. FADS2 also interacts with other proteins such as SCD1 (Stearoyl-CoA Desaturase 1) within these disorders further highlighting its role in lipid metabolism and associated diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Involved in the biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18 : 2n-6) and alpha-linolenic acid (ALA) (18 : 3n-3) precursors, acting as a fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 6 of the fatty acyl chain. Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18 : 2n-6) and ALA (18 : 3n-3) into gamma-linoleate (GLA) (18 : 3n-6) and stearidonate (18 : 4n-3), respectively (PubMed : 12713571). Subsequently, in the biosynthetic pathway of HUFA n-3 series, it desaturates tetracosapentaenoate (24 : 5n-3) to tetracosahexaenoate (24 : 6n-3), which is then converted to docosahexaenoate (DHA)(22 : 6n-3), an important lipid for nervous system function (By similarity). Desaturates hexadecanate (palmitate) to produce 6Z-hexadecenoate (sapienate), a fatty acid unique to humans and major component of human sebum, that has been implicated in the development of acne and may have potent antibacterial activity (PubMed : 12713571). It can also desaturate (11E)-octadecenoate (trans-vaccenoate, the predominant trans fatty acid in human milk) at carbon 6 generating (6Z,11E)-octadecadienoate (By similarity). In addition to Delta-6 activity, this enzyme exhibits Delta-8 activity with slight biases toward n-3 fatty acyl-CoA substrates (By similarity).

See full target information FADS2

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제품이 사용된 논문 (10)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of biological sciences 21:1275-1293 PubMed39897035

2025

Exosomal mediated lipid metabolism, ferritinophagy and CoQ-dependent pathway contributes to the ferroptosis of keratinocyte in SJS/TEN.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Zhang,Pei Qiao,ChunYing Xiao,ZiPeng Cao,JiaoLing Chen,Hui Fang,JianKang Yang,ZeHua Kang,ErLe Dang,Shuai Shao,BingYu Pang,QingYang Li,ZhenLai Zhu,ShengXian Shen,Akito Hasegawa,Riichiro Abe,HongJiang Qiao,Gang Wang,Meng Fu

Communications biology 7:787 PubMed38951550

2024

A single-cell sequence analysis of mouse subcutaneous white adipose tissue reveals dynamic changes during weaning.

Applications

Unspecified application

Species

Unspecified reactive species

Shuwen Qian,Chenyang Zhang,Yan Tang,Mengyuan Dai,Zhihui He,Hong Ma,Linyuan Wang,Qiqi Yang,Yang Liu,Wei Xu,Zhao Zhang,Qi-Qun Tang

Frontiers in molecular biosciences 11:1401558 PubMed38919749

2024

Cannabigerol-A useful agent restoring the muscular phospholipids milieu in obese and insulin-resistant Wistar rats?

Applications

Unspecified application

Species

Unspecified reactive species

Patrycja Bielawiec,Sylwia Dziemitko,Karolina Konstantynowicz-Nowicka,Klaudia Sztolsztener,Adrian Chabowski,Ewa Harasim-Symbor

Journal of personalized medicine 14: PubMed38673008

2024

Alteration of the Expression and Functional Activities of Myosin II Isoforms in Enlarged Hyperplastic Prostates.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Wang,Weixiang He,Hui Chen,Rui Yang,Hongmei Su,Michael E DiSanto,Xinhua Zhang

Journal of Cancer 14:1673-1688 PubMed37325064

2023

LncRNA LINC01569 promotes M2 macrophage polarization to accelerate hypopharyngeal carcinoma progression through the miR-193a-5p/FADS1 signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qilin Gong,Huaying Li,Jintian Song,Chang Lin

Frontiers in immunology 14:1074242 PubMed37122728

2023

Investigating the role of FADS family members in breast cancer based on bioinformatic analysis and experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Zhao,Pingping Gao,Yanling Li,Hao Tian,Dandan Ma,Na Sun,Ceshi Chen,Yi Zhang,Xiaowei Qi

Scientific reports 13:3694 PubMed36879113

2023

Cannabidiol improves muscular lipid profile by affecting the expression of fatty acid transporters and inhibiting de novo lipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Patrycja Bielawiec,Sylwia Dziemitko,Karolina Konstantynowicz-Nowicka,Adrian Chabowski,Janusz Dzięcioł,Ewa Harasim-Symbor

Clinical and translational medicine 12:e810 PubMed35560527

2022

Elevation of fatty acid desaturase 2 in esophageal adenocarcinoma increases polyunsaturated lipids and may exacerbate bile acid-induced DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey Molendijk,Cathryn M Kolka,Henry Cairns,Sandra Brosda,Ahmed Mohamed,Alok K Shah,Ian Brown,Mark P Hodson,Thomas Hennessy,Guanghao Liu,Thomas Stoll,Renee S Richards,Michael Gartside,Kalpana Patel,Nicholas J Clemons,Wayne A Phillips,Andrew Barbour,Johan A Westerhuis,Michelle M Hill

Transfusion 61:1867-1883 PubMed33904180

2021

Fatty acid desaturase activity in mature red blood cells and implications for blood storage quality.

Applications

Unspecified application

Species

Unspecified reactive species

Tiffany Thomas,Francesca Cendali,Xiaoyun Fu,Fabia Gamboni,Evan J Morrison,Jonathan Beirne,Travis Nemkov,Marianna H Antonelou,Anastasios Kriebardis,Ian Welsby,Ariel Hay,Karolina H Dziewulska,Michael P Busch,Steven Kleinman,Paul W Buehler,Steven L Spitalnik,James C Zimring,Angelo D'Alessandro

Journal of lipid research 61:1589-1604 PubMed32978274

2020

SCD1 promotes lipid mobilization in subcutaneous white adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zou,Yi-Na Wang,Hong Ma,Zhi-Hui He,Yan Tang,Liang Guo,Yang Liu,Meng Ding,Shu-Wen Qian,Qi-Qun Tang

제품이 사용된 논문 모두 보기

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Product promise

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