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AB230813

Anti-FAK (phospho Y925) antibody [EPR22330-75]

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(1 출판물)

Rabbit Recombinant Monoclonal FAK phospho Y925 antibody. Suitable for Dot, WB and reacts with Synthetic peptide, Human samples. Cited in 1 publication.

대체 명칭 보기

FAK, FAK1, PTK2, Focal adhesion kinase 1, FADK 1, Focal adhesion kinase-related nonkinase, Protein phosphatase 1 regulatory subunit 71, Protein-tyrosine kinase 2, p125FAK, pp125FAK, FRNK, PPP1R71

2 이미지
Western blot - Anti-FAK (phospho Y925) antibody [EPR22330-75] (AB230813)
  • WB

Unknown

Western blot - Anti-FAK (phospho Y925) antibody [EPR22330-75] (AB230813)

FAK can be cleaved at several sites. The molecular weight observed is consistent with what has been described in the literature (PMID : 10545505, 9642276).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-FAK (phospho Y925) antibody [EPR22330-75] (ab230813) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate (Untreated membrane) at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate (Phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 119 kDa

false

Exposure time: 70s

Dot Blot - Anti-FAK (phospho Y925) antibody [EPR22330-75] (AB230813)
  • Dot

Unknown

Dot Blot - Anti-FAK (phospho Y925) antibody [EPR22330-75] (AB230813)

Dot Blot - Anti-FAK (phospho Y925) antibody [EPR22330-75] (ab230813 at a 1/1000 dilution). Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as secondary antibody at 1/100000 dilution.

Lane 1 : FAK phospho Y925 peptide.

Lane 2 : FAK non-phospho peptide

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 mins.

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-FAK (phospho Y925) antibody [EPR22330-75] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22330-75

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB76244

Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-FAK (phospho Y576 + Y577) antibody [EP1832Y] (AB76244)

  • 0
  • 0
Primary Antibodies

AB76496

Anti-FAK antibody [EP1831Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP1831Y] (AB76496)

  • 0
  • 0
Primary Antibodies

AB40794

Anti-FAK antibody [EP695Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] (AB40794)

  • 5
  • 9
Primary Antibodies

AB271836

Anti-FAK antibody [EP695Y] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - BSA and Azide free (AB271836)

  • 0
  • 0
Primary Antibodies

AB271862

Anti-FAK (phospho Y397) antibody [EP2160Y] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-FAK (phospho Y397) antibody [EP2160Y] - BSA and Azide free (AB271862)

  • 0
  • 0
Primary Antibodies

AB81298

Anti-FAK (phospho Y397) antibody [EP2160Y]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

  • 4
  • 2

보완 제품

Primary Antibodies

AB81298

Anti-FAK (phospho Y397) antibody [EP2160Y]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-FAK (phospho Y397) antibody [EP2160Y] (AB81298)

  • 4
  • 2
Primary Antibodies

AB32084

Anti-Paxillin antibody [Y113]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Paxillin antibody [Y113] (AB32084)

  • 5
  • 21
Primary Antibodies

AB131435

Anti-FAK antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody (AB131435)

  • 5
  • 1
Primary Antibodies

AB30394

Anti-Integrin beta 1 antibody [12G10]

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 1 antibody [12G10] (AB30394)

  • 5
  • 9
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Reactivity 정보

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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Focal Adhesion Kinase (FAK) also known as Protein Tyrosine Kinase 2 (PTK2) is a non-receptor tyrosine kinase. This protein has a molecular weight of approximately 125 kDa. FAK is expressed at high levels in brain muscle and liver tissues. Mechanically FAK plays a role in cellular adhesion and migration by regulating integrin signaling and cell-extracellular matrix interactions. FAK auto-phosphorylates at tyrosine residue 397 creating a binding site for Src family kinases and promoting downstream signaling pathways.
Biological function summary

Focal Adhesion Kinase participates in the formation of focal adhesions which are complexes that connect the cytoskeleton to the extracellular matrix. The FAK protein functions as an important signaling node in these structures allowing for the assembly of multiprotein signal transduction complexes. FAK also controls cellular processes such as spreading motility and survival. The interaction with proteins such as Src kinases paxillin and talin facilitates its biological roles in cell signaling.

Pathways

Focal Adhesion Kinase engages in the regulation of the MAPK/ERK signaling pathway and the PI3K/AKT pathway. These pathways are instrumental for cell proliferation survival and migration. In these pathways FAK interacts with proteins such as PI3K Grb2 and Sos linking integrin-mediated signals with downstream effects that influence cell behavior and survival.

Altered FAK signaling has ties to cancer progression and metastasis as well as cardiovascular diseases. In cancer the overexpression of FAK and its interaction with proteins like Src and VEGFR can drive tumor growth and angiogenesis. In cardiovascular diseases improper FAK activation can lead to aberrant heart tissue remodeling and associated pathologies. Abnormalities in FAK signaling pathways can therefore contribute significantly to the development and progression of these diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Phosphorylates NEDD9 following integrin stimulation (PubMed : 9360983). Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.. Isoform 6. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
See full target information PTK2 pY925
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 11:972 PubMed33184263

2020

Exosome-derived ENO1 regulates integrin α6β4 expression and promotes hepatocellular carcinoma growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Keqiu Jiang,Chengyong Dong,Zeli Yin,Rui Li,Jiakai Mao,Chengye Wang,Junlin Zhang,Zhenming Gao,Rui Liang,Qi Wang,Liming Wang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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