AB74333

Anti-FAM175B antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal FAM175B antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ABRAXAS2.

2 이미지

Unknown

Immunoprecipitation - Anti-FAM175B antibody (AB74333)

ab74333 at 1 ug/ml (used for IP at 3 ug/lysate).
Lane 1 : Whole HeLa cell lysate at 1 mg for IP (20% of IP loaded).
Lane 2 : control IgG.
Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-FAM175B antibody (ab74333)

Predicted band size: 47 kDa

false

Unknown

Western blot - Anti-FAM175B antibody (AB74333)

Detection : Chemiluminescence with exposure times of 10 seconds

All lanes:

Western blot - Anti-FAM175B antibody (ab74333) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK293T whole cell lysate at 50 µg

Predicted band size: 47 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ABRAXAS2. The exact immunogen used to generate this antibody is proprietary information.

Q15018

Specificity

The antibody recognises a region between residue 350 and 390 of human FAM175B using the numbering given in entry NP_115558.3 (GeneID 23172).

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibody was affinity purified using an epitope specific to FAM175B immobilized on solid support.

보관 버퍼

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FAM175B also known as ABRAXAS2 is a protein with a molecular weight of approximately 42 kDa. It plays an important role in DNA repair processes. Expression of FAM175B is observed in various tissues with notable presence in the nucleus due to its involvement in DNA damage response. The protein has a significant presence in actively dividing cells where maintenance of genomic integrity is essential.

Biological function summary

FAM175B is part of the BRCA1 A complex which is an important player in homologous recombination repair. This complex helps in recognizing and repairing double-strand breaks in DNA a critical function for preventing genomic instability. FAM175B directly interacts with other components of this complex such as BRCA1 and RAP80 ensuring precise repair of DNA. The protein acts as a scaffold coordinating protein-protein interactions necessary for complex formation and function.

Pathways

FAM175B is an indispensable component of the DNA damage response and repair pathway. This pathway is paramount for cellular resistance to DNA-damaging agents. FAM175B functions alongside BRCA1 and other associated proteins like RAD51 to facilitate repair processes. It links the recognition of DNA lesions with the execution of DNA repair playing a pivotal role in maintaining cellular health.

FAM175B has a significant association with breast cancer susceptibility. Mutations or malfunctions within the BRCA1 A complex where FAM175B operates can escalate risks for developing breast cancer. Additionally FAM175B is indirectly connected to ovarian cancer through its interactions with BRCA1. Its dysfunction in these pathways contributes to impaired DNA repair leading to tumorigenesis in these tissues.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked polyubiquitin, leaving the last ubiquitin chain attached to its substrates (PubMed : 19214193, PubMed : 20032457, PubMed : 20656690, PubMed : 24075985). May act as a central scaffold protein that assembles the various components of the BRISC complex and retains them in the cytoplasm (PubMed : 20656690). Plays a role in regulating the onset of apoptosis via its role in modulating 'Lys-63'-linked ubiquitination of target proteins (By similarity). Required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1 (PubMed : 26195665). Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activities by enhancing its stability and cell surface expression (PubMed : 24075985, PubMed : 26344097). Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination (PubMed : 24075985). Required for normal induction of p53/TP53 in response to DNA damage (PubMed : 25283148). Independent of the BRISC complex, promotes interaction between USP7 and p53/TP53, and thereby promotes deubiquitination of p53/TP53, preventing its degradation and resulting in increased p53/TP53-mediated transcription regulation and p53/TP53-dependent apoptosis in response to DNA damage (PubMed : 25283148).

See full target information ABRAXAS2

대체 명칭 보기

ABRO1, FAM175B, KIAA0157, ABRAXAS2, BRISC complex subunit Abraxas 2, Abraxas brother protein 1, Protein FAM175B

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Leukemia 34:404-415 PubMed31576005

2019

Functional characterization of BRCC3 mutations in acute myeloid leukemia with t(8;21)(q22;q22.1).

Applications

Unspecified application

Species

Unspecified reactive species

Tatjana Meyer,Nikolaus Jahn,Stefanie Lindner,Linda Röhner,Anna Dolnik,Daniela Weber,Annika Scheffold,Simon Köpff,Peter Paschka,Verena I Gaidzik,Dirk Heckl,Sebastian Wiese,Benjamin L Ebert,Hartmut Döhner,Lars Bullinger,Konstanze Döhner,Jan Krönke

Molecular cell 75:483-497.e9 PubMed31253574

2019

Structural Basis of BRCC36 Function in DNA Repair and Immune Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Julius Rabl,Richard D Bunker,Andreas D Schenk,Simone Cavadini,Mark E Gill,Wassim Abdulrahman,Amparo Andrés-Pons,Martijn S Luijsterburg,Adel F M Ibrahim,Emma Branigan,Jacob D Aguirre,Aimee H Marceau,Claire Guérillon,Tewis Bouwmeester,Ulrich Hassiepen,Antoine H F M Peters,Martin Renatus,Laurent Gelman,Seth M Rubin,Niels Mailand,Haico van Attikum,Ronald T Hay,Nicolas H Thomä

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAM175B antibody

Immunoprecipitation - Anti-FAM175B antibody (AB74333)

Western blot - Anti-FAM175B antibody (AB74333)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Proteins & Peptides

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise