AB326598

Anti-FAM38A/PIEZO1 antibody [EPR30472-532] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal FAM38A/PIEZO1 antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Mouse, Mouse samples.

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Supplier Data

Western blot - Anti-FAM38A/PIEZO1 antibody [EPR30472-532] - BSA and Azide free (AB326598)

This data was developed using ab324311, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : skeletal muscle(PMID : 31263454).

Samples are non-boiled as boiling may cause protein aggregation.

This antibody was detected under gels with concentrations of 6% and 3-8% and it showed a significant difference in molecular weight.

In Western blot Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-FAM38A/PIEZO1 antibody [EPR30472-532] (<a href='/ko/products/primary-antibodies/fam38a-piezo1-antibody-epr30472-532-ab324311'>ab324311</a>) at 1/1000 dilution

Lanes 1 and 5:

MEF (mouse embryo fibroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lanes 2 and 6:

MEF transfected with siRNA specifically targeting FAM38A/PIEZO1 whole cell lysate at 20 µg

Lane 3:

Mouse lung tissue lysate at 20 µg

Lane 4:

Mouse skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 300 kDa,124 kDa

false

Exposure time: 103s

Supplier Data

Western blot - Anti-FAM38A/PIEZO1 antibody [EPR30472-532] - BSA and Azide free (AB326598)

This data was developed using ab324311, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed mouse PIEZO2 by western blot.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-FAM38A/PIEZO1 antibody [EPR30472-532] (<a href='/ko/products/primary-antibodies/fam38a-piezo1-antibody-epr30472-532-ab324311'>ab324311</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse FAM38A/PIEZO1 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse PIEZO2 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 250-350 kDa,124 kDa,250-300 kDa

false

Exposure time: 1s

Supplier Data

Western blot - Anti-FAM38A/PIEZO1 antibody [EPR30472-532] - BSA and Azide free (AB326598)

This data was developed using ab324311, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : skeletal muscle(PMID : 31263454).

Samples are non-boiled as boiling may cause protein aggregation.

This blot was developed using a high-sensitivity ECL substrate allowing for the detection of proteins in the mid-femtogram range.

In Western blot Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-FAM38A/PIEZO1 antibody [EPR30472-532] (<a href='/ko/products/primary-antibodies/fam38a-piezo1-antibody-epr30472-532-ab324311'>ab324311</a>) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 40 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 40 µg

Lane 3:

Mouse heart tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 300 kDa,124 kDa

true

Exposure time: 180s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30472-532

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab326598 is the carrier-free version of ab324311

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Pore-forming subunit of the mechanosensitive non-specific cation Piezo channel required for rapidly adapting mechanically activated (MA) currents and has a key role in sensing touch and tactile pain (PubMed : 20813920, PubMed : 22343900, PubMed : 38184690, PubMed : 26390154, PubMed : 29261642, PubMed : 35388220, PubMed : 37590348). Piezo channels are homotrimeric three-blade propeller-shaped structures that utilize a cap-motion and plug-and-latch mechanism to gate their ion-conducting pathways (PubMed : 26390154, PubMed : 29261642, PubMed : 35388220, PubMed : 37590348). Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium (PubMed : 20813920, PubMed : 22343900, PubMed : 38228630). Conductance to monovalent alkali ions is highest for K(+), intermediate for Na(+) and lowest for Li(+) (By similarity). Divalent ions except for Mn(2+) permeate the channel but more slowly than the monovalent ions and they also reduce K(+) currents (By similarity). Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling (By similarity). In inner ear hair cells, PIEZO1/2 subunits may constitute part of the mechanotransducer (MET) non-selective cation channel complex where they may act as pore-forming ion-conducting component in the complex (PubMed : 38228630). In the kidney, may contribute to the detection of intraluminal pressure changes and to urine flow sensing (PubMed : 24157948). Acts as a shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation (By similarity). Plays a key role in blood vessel formation and vascular structure in both development and adult physiology (PubMed : 24958852, PubMed : 25119035, PubMed : 36515266). Acts as a sensor of phosphatidylserine (PS) flipping at the plasma membrane and governs morphogenesis of muscle cells (PubMed : 29799007). In myoblasts, flippase-mediated PS enrichment at the inner leaflet of plasma membrane triggers channel activation and Ca(2+) influx followed by Rho GTPases signal transduction, leading to assembly of cortical actomyosin fibers and myotube formation (PubMed : 29799007).

See full target information Piezo1

대체 명칭 보기

Fam38a, Piezo-type mechanosensitive ion channel component 1, Protein FAM38A

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAM38A/PIEZO1 antibody [EPR30472-532] - BSA and Azide free

Western blot - Anti-FAM38A/PIEZO1 antibody [EPR30472-532] - BSA and Azide free (AB326598)

Western blot - Anti-FAM38A/PIEZO1 antibody [EPR30472-532] - BSA and Azide free (AB326598)

Western blot - Anti-FAM38A/PIEZO1 antibody [EPR30472-532] - BSA and Azide free (AB326598)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise