AB236882

Anti-FAM83D antibody

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(4 제품이 사용된 논문 )

Rabbit Polyclonal FAM83D antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FAM83D aa 300 to C-terminus.

대체 명칭 보기

C20orf129, FAM83D, Protein FAM83D, Spindle protein CHICA

1 이미지

Immunocytochemistry/ Immunofluorescence - Anti-FAM83D antibody (AB236882)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FAM83D antibody (AB236882)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for FAM83D (green) using ab236882 at a dilution of 1/133 in ICC/IF. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FAM83D aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H4H8

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FAM83D also known as Family with Sequence Similarity 83 Member D is an important protein involved in cellular processes. It is a protein of significant mass approximately 68 kDa and is highly expressed in the nucleus of proliferating cells. FAM83D's role is associated with cell cycle regulation and it functions effectively in maintaining proper chromosome segregation during mitosis. The protein shows higher expression in rapidly dividing tissues suggesting a link to cell division and growth mechanisms.

Biological function summary

FAM83D participates in vital cellular functions related to mitosis and cancer cell proliferation. It does not function alone but is part of the spindle assembly complex ensuring proper mitotic spindle formation and stability. These interactions highlight its importance in maintaining genomic integrity during cell division reducing the potential for errors that can lead to tumorigenesis. The involvement in this complex makes FAM83D an attractive target for studying cancer biology.

Pathways

FAM83D interacts closely with several key proteins governing cell cycle progression including the Aurora kinase pathway and the Plk1 pathway. It plays a role in phosphorylating and activating proteins necessary for mitotic entry and progression. FAM83D's interaction with Aurora B kinase is particularly critical as it contributes to proper chromosomal alignment and segregation linking it to important regulatory mechanisms in cell proliferation.

Aberrant expression of FAM83D contributes to cancer development including breast cancer and colorectal cancer. Abnormal FAM83D levels disrupt normal cell cycle regulation leading to uncontrolled cell growth. Additionally its interaction with cancer-related proteins such as Cyclin B1 highlights its significance in malignant cells offering potential pathways for therapeutic intervention. Understanding FAM83D's roles and relationships may offer insight into diagnostic and treatment strategies for these cancers.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Through the degradation of FBXW7, may act indirectly on the expression and downstream signaling of MTOR, JUN and MYC (PubMed : 24344117). May play also a role in cell proliferation through activation of the ERK1/ERK2 signaling cascade (PubMed : 25646692). May also be important for proper chromosome congression and alignment during mitosis through its interaction with KIF22 (PubMed : 18485706).

See full target information FAM83D

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Translational oncology 22:101454 PubMed35617811

2022

Suppression of FAM83D Inhibits Glioma Proliferation, Invasion and Migration by Regulating the AKT/mTOR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Li,Cui Sun,Jing Chen,Ji-Fen Ma,Yi-Heng Pan

Yonsei medical journal 63:282-291 PubMed35184431

2022

MiR-322-5p Alleviates Cell Injury and Impairment of Cognitive Function in Vascular Dementia by Targeting TSPAN5.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zheng,Jie Zhang,Bin Zhou,Huanxian Chang

OncoTargets and therapy 13:4665-4678 PubMed32547096

2020

Knockdown of FAM83D Enhances Radiosensitivity in Coordination with Irradiation by Inhibiting EMT via the Akt/GSK-3β/Snail Signaling Pathway in Human Esophageal Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xing-Xiao Yang,Ming Ma,Mei-Xiang Sang,Xue-Yuan Zhang,Nai-Yi Zou,Shu-Chai Zhu

Journal of experimental & clinical cancer research 39:55 PubMed32228656

2020

circFOXM1 promotes proliferation of non-small cell lung carcinoma cells by acting as a ceRNA to upregulate FAM83D.

Applications

Unspecified application

Species

Unspecified reactive species

Chengtao Yu,Zhuoan Cheng,Shaohua Cui,Xiaowei Mao,Botai Li,Yujie Fu,Hui Wang,Haojie Jin,Qing Ye,Xiaojing Zhao,Liyan Jiang,Wenxin Qin

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAM83D antibody

Immunocytochemistry/ Immunofluorescence - Anti-FAM83D antibody (AB236882)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise