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AB109542

Anti-FANCD2 (phospho S1404) antibody [EPR2278(2)]

4

(1 리뷰)

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal FANCD2 phospho S1404 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.
1 이미지
Western blot - Anti-FANCD2 (phospho S1404) antibody [EPR2278(2)] (AB109542)
  • WB

Unknown

Western blot - Anti-FANCD2 (phospho S1404) antibody [EPR2278(2)] (AB109542)

The lower panel is analysed using an alternative anti-FANCD2 antibody which is not phospho-specific.

All lanes:

Western blot - Anti-FANCD2 (phospho S1404) antibody [EPR2278(2)] (ab109542) at 1/1000 dilution

Lane 1:

HeLa cell lysate, untreated at 10 µg

Lane 2:

HeLa cell lysate treated with Alkaline Phosphatase. at 10 µg

Secondary

All lanes:

HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 166 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-FANCD2 (phospho S1404) antibody [EPR2278(2)] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2278(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB200763

Alexa Fluor® 647 Anti-FANCD2 antibody [EPR2302]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FANCD2 antibody [EPR2302] (AB200763)

  • 0
  • 0
Primary Antibodies

AB108928

Anti-FANCD2 antibody [EPR2302]

20ul selling size|KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-FANCD2 antibody [EPR2302] (AB108928)

  • 5
  • 4
Primary Antibodies

AB178705

Anti-FANCD2 antibody [EPR2279(3)]

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-FANCD2 antibody [EPR2279(3)] (AB178705)

  • 5
  • 1
Primary Antibodies

AB245347

Anti-FANCD2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCD2 antibody (AB245347)

  • 0
  • 0
Primary Antibodies

AB200759

Alexa Fluor® 488 Anti-FANCD2 antibody [EPR2302]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-FANCD2 antibody [EPR2302] (AB200759)

  • 0
  • 0
Primary Antibodies

AB221932

Anti-FANCD2 antibody [EPR2302] - BSA and Azide free

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-FANCD2 antibody [EPR2302] - BSA and Azide free (AB221932)

  • 3
  • 1

보완 제품

Primary Antibodies

AB108928

Anti-FANCD2 antibody [EPR2302]

20ul selling size|KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-FANCD2 antibody [EPR2302] (AB108928)

  • 5
  • 4
Primary Antibodies

AB306583

Anti-TMEM119 antibody [EPR25865-89] - Microglial marker

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM119 antibody [EPR25865-89] - Microglial marker (AB306583)

  • 0
  • 0
Primary Antibodies

AB124918

Anti-DNA PKcs (phospho S2056) antibody [EPR5670]

RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-DNA PKcs (phospho S2056) antibody [EPR5670] (AB124918)

  • 5
  • 1
Primary Antibodies

AB76420

Anti-RPA32/RPA2 antibody [EPR2877Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FANCD2 also known as Fanconi anemia group D2 functions mechanically in DNA repair processes. This protein plays a role in the cellular response to DNA damage specifically interstrand crosslinks. FANCD2 has a molecular weight of approximately 164 kDa. Expression of FANCD2 occurs broadly in various tissues but reaches higher levels in cells that undergo rapid division such as hematopoietic cells.
Biological function summary

FANCD2 collaborates with multiple proteins as part of the Fanconi anemia (FA) complex to ensure genomic stability. The FA complex operates by facilitating the repair of DNA interstrand crosslinks that impede replication. FANCD2 becomes activated through monoubiquitination which serves as a signal for recruitment to DNA damage sites. It essentially functions as a coordinator that brings together important components for repair.

Pathways

FANCD2 integrates into the Fanconi anemia pathway and the homologous recombination repair pathway. These pathways are critical for maintaining DNA integrity and preventing chromosomal instability. Within these pathways interactions with proteins such as BRCA1 and BRCA2 reinforce the repair processes. FANCD2's connection to these proteins exemplifies its role in complex mechanisms that preserve genomic fidelity.

FANCD2 links significantly to Fanconi anemia a genetic disorder that causes bone marrow failure and increased cancer susceptibility. Damage or malfunction of FANCD2 can disrupt DNA repair leading to cellular dysfunction seen in this condition. Additionally there is a connection to breast cancer whereby FANCD2 interacts with BRCA2 indicating a shared pathway involved in tumor suppressor functions. These associations underline the importance of FANCD2 in disease pathology and its potential as a therapeutic target.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Required for maintenance of chromosomal stability (PubMed : 11239453, PubMed : 14517836). Promotes accurate and efficient pairing of homologs during meiosis (PubMed : 14517836). Involved in the repair of DNA double-strand breaks, both by homologous recombination and single-strand annealing (PubMed : 15671039, PubMed : 15650050, PubMed : 30335751, PubMed : 36385258). The FANCI-FANCD2 complex binds and scans double-stranded DNA (dsDNA) for DNA damage; this complex stalls at DNA junctions between double-stranded DNA and single-stranded DNA (By similarity). May participate in S phase and G2 phase checkpoint activation upon DNA damage (PubMed : 15377654). Plays a role in preventing breakage and loss of missegregating chromatin at the end of cell division, particularly after replication stress (PubMed : 15454491, PubMed : 15661754). Required for the targeting, or stabilization, of BLM to non-centromeric abnormal structures induced by replicative stress (PubMed : 15661754, PubMed : 19465921). Promotes BRCA2/FANCD1 loading onto damaged chromatin (PubMed : 11239454, PubMed : 12239151, PubMed : 12086603, PubMed : 15115758, PubMed : 15199141, PubMed : 15671039, PubMed : 18212739). May also be involved in B-cell immunoglobulin isotype switching.
See full target information FANCD2 pS1404

대체 명칭 보기

FACD, FANCD2, Fanconi anemia group D2 protein, Protein FACD2
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록and refine your search

Environmental and molecular mutagenesis 61:534-550 PubMed32297368

2020

Image analysis of mechanistic protein biomarkers for the characterization of genotoxicants: Aneugens, clastogens, and reactive oxygen species inducers.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Wilde,Nina Queisser,Andreas Sutter

Proteomics 18:e1700242 PubMed29460479

2018

Label-Free Quantitative Phosphoproteomics Reveals Regulation of Vasodilator-Stimulated Phosphoprotein upon Stathmin-1 Silencing in a Pair of Isogenic Colorectal Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Hwee Tong Tan,Maxey Ching Ming Chung
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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