Anti-Fas antibody

• IP

Supplier Data

Immunoprecipitation - Anti-Fas antibody (AB245450)

Fas was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245450 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab245450 at 1 μg/ml.

Lane 1 : ab245450 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Fas antibody (ab245450)

Predicted band size: 37 kDa

false

• WB

Supplier Data

Western blot - Anti-Fas antibody (AB245450)

All lanes:

Western blot - Anti-Fas antibody (ab245450) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 37 kDa

true

Exposure time: 3min