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AB240956

Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free

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(1 출판물)

Rabbit Recombinant Monoclonal FBX32 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 1 publication.
2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (AB240956)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (AB240956)

Immunofluorescent staining of A673 cells (fixed in 4% PFA) using purified ab168372 at a dilution of 1/200. An Alexa Fluor® 555 goat anti-rabbit (ab150082) was used at a dilution of 1/500 and the cells were counter-stained with DAPI. The negative control is shown in the bottom right hand panel - for the negative control, purified ab168372 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/400.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168372)

Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (AB240956)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Fbx32 antibody [EPR9148(2)] - BSA and Azide free (AB240956)

Immunofluorescent analysis of A673 cells labeling Fbx32 with ab168372 at 1/250 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab168372)

관련 conjugated 항체와 다양한 조성의 항체 (2)

  • Unconjugated

    Anti-Fbx32 antibody [EPR9148(2)]

  • HRP

    HRP Anti-Fbx32 antibody [EPR9148(2)]

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9148(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB157596

Anti-Fbx32 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fbx32 antibody (AB157596)

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  • 0
Primary Antibodies

AB92281

Anti-Fbx32 antibody

Western blot - Anti-Fbx32 antibody (AB92281)

  • 0
  • 0
Primary Antibodies

AB74023

Anti-Fbx32 antibody

Western blot - Anti-Fbx32 antibody (AB74023)

  • 5
  • 6

보완 제품

Sample Prep & Detection Kits

AB269896

Atto 488 Conjugation Kit (Fast) - Lightning-Link®

Immunocytochemistry - Atto 488 Conjugation Kit (Fast) - Lightning-Link® (AB269896)

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Sample Prep & Detection Kits

AB186922

Biotin Gold Conjugate (40nm, 10 OD)

Functional Studies - Biotin Gold Conjugate (40nm, 10 OD) (AB186922)

  • 0
  • 0
Primary Antibodies

AB172479

Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (AB172479)

  • 0
  • 1
Primary Antibodies

AB124800

Anti-Myogenin antibody [EPR4789]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myogenin antibody [EPR4789] (AB124800)

  • 5
  • 3
Primary Antibodies

AB191838

Anti-PGC1 alpha antibody

Western blot - Anti-PGC1 alpha antibody (AB191838)

  • 5
  • 2
Primary Antibodies

AB32047

Anti-AMPK alpha 1 antibody [Y365]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-AMPK alpha 1 antibody [Y365] (AB32047)

  • 5
  • 6
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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제품 세부 정보

ab240956 is the carrier-free version of ab168372.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1. Negatively regulates macrophage efferocytosis by promoting the ubiquitination of the transcription factor KLF4, suppressing the receptor tyrosine kinase MERTK transcription (By similarity). Regulates LPS-induced apoptosis and mitochondrial dysfunction through ANXA1 ubiquitination and degradation (By similarity). Mediates cyclin D1 protein stabilization via 'Lys-27'-linked ubiquitination (PubMed : 40307251).
See full target information F-box only protein 32

대체 명칭 보기

F-box only protein 32, Atrogin-1, Muscle atrophy F-box protein, MAFbx, FBXO32
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports 28:1690-1702.e10 PubMed31412240

2019

SMUG1 Promotes Telomere Maintenance through Telomerase RNA Processing.

Applications

Unspecified application

Species

Unspecified reactive species

Penelope Kroustallaki,Lisa Lirussi,Sergio Carracedo,Panpan You,Q Ying Esbensen,Alexandra Götz,Laure Jobert,Lene Alsøe,Pål Sætrom,Sarantis Gagos,Hilde Nilsen
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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