AB223712

Anti-FCN1/M-Ficolin antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal FCN1/M-Ficolin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FCN1 aa 1-150.

대체 명칭 보기

FCNM, FCN1, Ficolin-1, Collagen/fibrinogen domain-containing protein 1, Ficolin-A, Ficolin-alpha, M-ficolin

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCN1/M-Ficolin antibody (AB223712)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCN1/M-Ficolin antibody (AB223712)

Paraffin embedded sections of human bone marrow tissue were stained for FCN1/M-Ficolin with ab223712 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCN1/M-Ficolin antibody (AB223712)

Paraffin embedded sections of human small intestine tissue were stained for FCN1/M-Ficolin with ab223712 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCN1/M-Ficolin antibody (AB223712)

Paraffin embedded sections of human lymph node tissue were stained for FCN1/M-Ficolin with ab223712 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-FCN1/M-Ficolin antibody (AB223712)

All lanes:

Western blot - Anti-FCN1/M-Ficolin antibody (ab223712) at 1/250 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Lane 3:

A431 (human epidermoid carcinoma cell line) cell lysate

Lane 4:

Human liver lysate

Predicted band size: 35 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human FCN1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O00602

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FCN1 also known as M-Ficolin or ficolin-1 is a pattern recognition molecule part of the innate immune system. It plays a role in recognizing pathogen-associated molecular patterns (PAMPs) on the surface of microbes. FCN1 is a 35 kDa protein predominantly expressed in the liver and secreted into the bloodstream. It consists of a collagen-like domain and a fibrinogen-like recognition domain allowing it to effectively bind its targets. The protein occurs in plasma where it particularly acts in host defense mechanisms.

Biological function summary

FCN1 functions as part of the body’s first line of immune defense by binding to microbial surfaces facilitating the opsonization and activation of the lectin complement pathway. It is a member of a complex known as the lectin pathway of complement activation which includes MASP1 and MASP2 proteins. FCN1 triggers this pathway when it recognizes patterns such as N-acetylglucosamine on the surface of pathogens which is important for initiating the immune response and promoting pathogen clearance through complement activation in cooperation with other components of the pathway.

Pathways

FCN1 primarily participates in the lectin complement pathway distinctively different from the classical and alternative pathways. It interacts with MASP proteins to trigger the cleavage of complement proteins which leads to a cascade that results in inflammation opsonization and pathogen lysis. FCN1 connections also extend to other pattern recognition receptors such as those in the toll-like receptor pathway which harmonize to ensure a robust immune response by recognizing wide array of pathogen motifs.

FCN1 involvement has been observed in several conditions notably systemic lupus erythematosus (SLE) and bacterial infections. Alterations or deficiencies in FCN1 levels can influence susceptibility to infections due to compromised opsonization and pathogen clearance. In SLE FCN1 and its associated pathway proteins may contribute to the disease pathogenesis by affecting complement system activity leading to autoimmune and inflammatory responses. Understanding these associations aids in unraveling the complex network of immune response regulation and disease development.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Extracellular lectin, which acts as a pattern recognition receptor that initiates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 16116205, PubMed : 17897951, PubMed : 20032467). Specifically recognizes and binds carbohydrates on the pathogen surface, activating the MASP1 serine protease and initiating the proteolytic cascade of the lectin complement pathway (PubMed : 16116205, PubMed : 20032467). Binds preferentially to 9-O-acetylated 2-6-linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage (PubMed : 20032467, PubMed : 20400674). May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8 (PubMed : 21037097).

See full target information FCN1

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular cancer 24:102 PubMed40165182

2025

C1Q TPP1 macrophages promote colon cancer progression through SETD8-driven p53 methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Veronica Veschi,Francesco Verona,Sebastiano Di Bella,Alice Turdo,Miriam Gaggianesi,Simone Di Franco,Laura Rosa Mangiapane,Chiara Modica,Melania Lo Iacono,Paola Bianca,Ornella Roberta Brancato,Caterina D'Accardo,Gaetana Porcelli,Vincenzo Luca Lentini,Isabella Sperduti,Elisabetta Sciacca,Peter Fitzgerald,David Lopez-Perez,Pierre Martine,Kate Brown,Giuseppe Giannini,Ettore Appella,Giorgio Stassi,Matilde Todaro

The Journal of clinical investigation 133: PubMed36928117

2023

Keratinocytes sense and eliminate CRISPR DNA through STING/IFN-κ activation and APOBEC3G induction.

Applications

Unspecified application

Species

Unspecified reactive species

Mrinal K Sarkar,Ranjitha Uppala,Chang Zeng,Allison C Billi,Lam C Tsoi,Austin Kidder,Xianying Xing,Bethany E Perez White,Shuai Shao,Olesya Plazyo,Sirisha Sirobhushanam,Enze Xing,Yanyun Jiang,Katherine A Gallagher,John J Voorhees,J Michelle Kahlenberg,Johann E Gudjonsson

Signal transduction and targeted therapy 8:44 PubMed36710358

2023

Single-cell RNA-sequencing reveals radiochemotherapy-induced innate immune activation and MHC-II upregulation in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Liu,Xiaohui Li,Qingyu Huang,Min Zhang,Tianyu Lei,Fuhao Wang,Wenxue Zou,Rui Huang,Xiaoyu Hu,Cong Wang,Xiaoling Zhang,Bing Sun,Ligang Xing,Jinbo Yue,Jinming Yu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Anti-FCN1/M-Ficolin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCN1/M-Ficolin antibody (AB223712)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCN1/M-Ficolin antibody (AB223712)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FCN1/M-Ficolin antibody (AB223712)

Western blot - Anti-FCN1/M-Ficolin antibody (AB223712)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise