AB321969

Anti-FCRL5 antibody [EPR26948-187] - BSA and Azide free

Recombinant
RabMAb
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(0 출판물)

Rabbit Recombinant Monoclonal FCRL5 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.

4 이미지

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] - BSA and Azide free (AB321969)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] - BSA and Azide free (AB321969)

This data was developed using ab321968, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Daudi (human Burkitt's lymphoma lymphoblast) (Right) / MOLT-4 (human lymphoblastic leukemia T lymphoblast) (Left) cells labelling FCRL5 with ab321968 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Negative control : MOLT-4 (PMID : 11290337).
Cross-reactivity with FCRL1 but no cross-reactivity with FCRL2, FCRL3, FCRL4 and FCRL6.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] - BSA and Azide free (AB321969)

This data was developed using ab321968, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling FCRL5 with ab321968 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD3 conjugated to Brilliant Violet 421. Gated on viable Lymphocytes.
Cross-reactivity with FCRL1 but no cross-reactivity with FCRL2, FCRL3, FCRL4 and FCRL6.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] - BSA and Azide free (AB321969)

This data was developed using ab321968, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of NAMALWA (human Burkitt's lymphoma B lymphocyte) (Right) / Jurkat (human T cell leukemia T lymphocyte from peripheral blood) (Left) cells labelling FCRL5 with ab321968 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Negative control : Jurkat (PMID : 24504816).
Cross-reactivity with FCRL1 but no cross-reactivity with FCRL2, FCRL3, FCRL4 and FCRL6.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] - BSA and Azide free (AB321969)

This data was developed using ab321968, the same antibody clone in a different buffer formulation.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti human CD19 conjugated to Alexa Fluor 647. Gated on viable Lymphocytes.
Cross-reactivity with FCRL1 but no cross-reactivity with FCRL2, FCRL3, FCRL4 and FCRL6.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26948-187

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

style

left-column

제품 세부 정보

ab321969 is the carrier-free version of ab321968.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The FCRL5 also known as CD307 or Fc receptor-like 5 is a protein predominantly expressed on the surface of B cells and plasma cells. This protein weighs approximately 98 kDa. FCRL5 features structural similarities to classical Fc receptors suggesting a role in immune responses. Its expression is tightly regulated throughout B cell development and has been detected in lymphoid tissues with higher levels reported in bone marrow and secondary lymphoid organs.

Biological function summary

FCRL5 modulates the immune system by influencing B cell activation and differentiation. It participates in the formation of immune complexes playing a dual role in both promoting and inhibiting immune responses depending on the surrounding conditions. This regulatory function suggests that FCRL5 can adjust immune system activities to maintain homeostasis. It acts independently and might form complexes with other immune regulatory proteins to fine-tune B cell responses.

Pathways

FCRL5 plays a role in the adaptive immune response and is associated with the B cell receptor (BCR) signaling pathway. It interacts with proteins such as CD19 and CD79 influencing downstream signaling cascades critical for B cell maturation and effector functions. The interaction of FCRL5 with these pathways highlights its importance in modulating signal transduction that leads to cytokine production and antibody generation.

FCRL5 has been implicated in the pathogenesis of autoimmune diseases and certain hematological cancers such as chronic lymphocytic leukemia (CLL). Abnormal expression or mutations of FCRL5 can dysregulate normal B cell function contributing to disease development. In CLL FCRL5 potentially interacts with proteins like leukocyte-associated immunoglobulin-like receptor 1 (LAIR1) influencing cancer cell survival and proliferation making it a target for therapeutic intervention and a potential biomarker for disease progression.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Plays an important role in B-cell response to antigen that acts both as a negative or positive coreceptor. Inhibits B-cell receptor (BCR) signaling in the absence of CR2 stimulation but engagement with CR2 and the BCR lead to a superior calcium response compared to CR2 and BCR costimulation (PubMed : 30107486). May be involved in B-cell development and differentiation in peripheral lymphoid organs and may be useful markers of B-cell stages. May have an immunoregulatory role in marginal zone B-cells. May play a role in fertilization (By similarity).

See full target information FCRL5

대체 명칭 보기

CD307e, FCRH5, IRTA2, UNQ503/PRO820, FCRL5, Fc receptor-like protein 5, FcR-like protein 5, FcRL5, BXMAS1, Fc receptor homolog 5, Immune receptor translocation-associated protein 2, FcRH5

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FCRL5 antibody [EPR26948-187] - BSA and Azide free

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] - BSA and Azide free (AB321969)

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] - BSA and Azide free (AB321969)

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] - BSA and Azide free (AB321969)

Flow Cytometry - Anti-FCRL5 antibody [EPR26948-187] - BSA and Azide free (AB321969)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise