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AB325416

Anti-FGF 23 antibody [EPR25947-29] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal FGF 23 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat samples.
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Sandwich ELISA - Anti-FGF 23 antibody [EPR25947-29] - BSA and Azide free (Detector) (AB325416)
  • sELISA

Lab

Sandwich ELISA - Anti-FGF 23 antibody [EPR25947-29] - BSA and Azide free (Detector) (AB325416)

Interpolated concentration of native FGF 23 was measured at different sample concentrations. Undiluted samples are as follows : mouse serum 100%, mouse plasma 100% and rat plasma 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-FGF 23 antibody [EPR25947-29] - BSA and Azide free (Detector) (AB325416)
  • sELISA

Lab

Sandwich ELISA - Anti-FGF 23 antibody [EPR25947-29] - BSA and Azide free (Detector) (AB325416)

Sandwich ELISA with the capture antibody ab325415 dilution at 2 µg/mL and detector antibody ab325416 dilution at 0.5 µg/mL.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25947-29

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

제품 세부 정보

ab325416 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse,Rat FGF 23. The recommended pair for sandwich ELISA is:
Capture: ab325415, Mouse,Rat FGF 23 Capture Antibody (unconjugated)
Detector: ab325416, Mouse,Rat FGF 23 Detector Antibody (unconjugated)
The reference range value is 19.5-1250 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Regulator of phosphate homeostasis (By similarity). Inhibits renal tubular phosphate transport by reducing SLC34A1 levels (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism (By similarity). Negatively regulates osteoblasts differentiation and matrix mineralization (By similarity). Up-regulates EGR1 expression in the presence of KL (PubMed : 17086194).
See full target information Fgf23

대체 명칭 보기

Fibroblast growth factor 23, FGF-23, Fgf23

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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