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AB281097

Anti-FGFR4 antibody [EPR23894-15] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal FGFR4 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
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Sandwich ELISA - Anti-FGFR4 antibody [EPR23894-15] - BSA and Azide free (Detector) (AB281097)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-FGFR4 antibody [EPR23894-15] - BSA and Azide free (Detector) (AB281097)

Example of Mouse FGFR4 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23894-15

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB256399

Human FGFR-4 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human FGFR-4 ELISA Kit (AB256399)

  • 0
  • 0
Sample Prep & Detection Kits

AB201802

DyLight® 633 Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - DyLight® 633 Conjugation Kit (Fast) - Lightning-Link® (AB201802)

  • 0
  • 0
Sample Prep & Detection Kits

AB269894

Latex Conjugation Kit - 400nm Black

Functional Studies - Latex Conjugation Kit - 400nm Black (AB269894)

  • 0
  • 0
Primary Antibodies

AB242636

Anti-FGFR3 antibody [EPR19020-170] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-FGFR3 antibody [EPR19020-170] - BSA and Azide free (Capture) (AB242636)

  • 0
  • 0
Primary Antibodies

AB192589

Anti-FGFR4 (phospho Y642) antibody

Western blot - Anti-FGFR4 (phospho Y642) antibody (AB192589)

  • 0
  • 0
Primary Antibodies

AB244981

Anti-FGF19 antibody [EPR20803-221] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-FGF19 antibody [EPR20803-221] - BSA and Azide free (Detector) (AB244981)

  • 0
  • 0
Primary Antibodies

AB59194

Anti-FGFR1 (phospho Y654) antibody

BOND RX™ Validated

Western blot - Anti-FGFR1 (phospho Y654) antibody (AB59194)

  • 4
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab281097 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse FGFR4. The recommended pair for sandwich ELISA is:
Capture: ab281247, Mouse FGFR4 Capture Antibody (unconjugated)
Detector: ab281097, Mouse FGFR4 Detector Antibody (unconjugated)
The reference range value is 156 - 10000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FGFR4 or fibroblast growth factor receptor 4 is a receptor tyrosine kinase weighing approximately 110 kDa. This protein plays a role in cellular processes by binding to fibroblast growth factors. FGFR4 is expressed in many tissues including muscle liver and the developing epidermis. It is known by alternate names like CD334 or FRFR4. This receptor's activation leads to receptor dimerization and autophosphorylation triggering downstream signaling pathways that affect cell proliferation differentiation and migration.
Biological function summary

FGFR4 influences various developmental and metabolic processes. It is important in limb and skeletal development epithelial cell morphogenesis and muscle fiber regeneration. FGFR4 often partners with heparan sulfate proteoglycans to form a signaling complex which defines the specificity and regulation of fibroblast growth factor signaling. The receptor's interaction with other proteins like FRFR4 and 5b5 further modulates its biological activity.

Pathways

FGFR4 participates in the MAPK and PI3K-Akt signaling pathways both important for regulating cell survival and growth. These pathways allow FGFR4 to connect with several other proteins such as FGFR1 and BLU-554. FGFR4's activation within these pathways helps determine cellular responses to hormonal and mitogenic signals further influencing tissue homeostasis and repair mechanisms.

FGFR4 is linked to certain cancers including hepatocellular carcinoma and colorectal cancer notable in cell lines like HCT116 and others. Overexpression or mutations in FGFR4 can drive tumor progression promoting uncontrolled growth and metastasis. FGFR4's relation to other key proteins such as FRAF and 19h3 highlights its role in pathogenesis making it a potential target for therapeutic interventions. Studies involving FGFR4 ELISA kits enhance understanding of its role in disease development and progression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Plays a role in postnatal lung development. May be involved in the development of skeletal muscle cell lineages.
See full target information Fgfr4

대체 명칭 보기

CD334, Fgfr-4, Mpk-11, Fgfr4, Fibroblast growth factor receptor 4, FGFR-4, Protein-tyrosine kinase receptor MPK-11
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Product promise

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