JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB299

Anti-Fibronectin antibody

5

(2 리뷰들)

|

(22 제품이 사용된 논문 )

Rabbit Polyclonal Fibronectin antibody. Suitable for sELISA, ICC/IF and reacts with Human samples. Cited in 22 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human FN1.

대체 명칭 보기

FN, FN1, Fibronectin, Cold-insoluble globulin, CIG

1 이미지
Sandwich ELISA - Anti-Fibronectin antibody (AB299)
  • sELISA

Unknown

Sandwich ELISA - Anti-Fibronectin antibody (AB299)

Standard Curve for Fibronectin (Analyte : Fibronectin protein (Human) (ab81743)); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal [HFN 7.1] to Fibronectin - BSA and Azide free (ab80923) at 1μg/ml and Detector Antibody Rabbit polyclonal to Fibronectin (ab299) at 0.5μg/ml.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, sELISA

applications

Immunogen

Native Full Length Protein corresponding to Human FN1.

P02751

Specificity

<p>Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.</p>

style
left-column

대체품

Primary Antibodies

AB45688

Anti-Fibronectin antibody [F14]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F14] (AB45688)

  • 5
  • 5
Primary Antibodies

AB198934

Alexa Fluor® 647 Anti-Fibronectin antibody [F1]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Fibronectin antibody [F1] (AB198934)

  • 0
  • 0
Primary Antibodies

AB206928

Anti-Fibronectin antibody [F14] - BSA and Azide free

KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F14] - BSA and Azide free (AB206928)

  • 0
  • 0
Primary Antibodies

AB207628

HRP Anti-Fibronectin antibody [F14]

RabMAb|Recombinant

Western blot - HRP Anti-Fibronectin antibody [F14] (AB207628)

  • 0
  • 0
Primary Antibodies

AB271831

Anti-Fibronectin antibody [F1] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1] - BSA and Azide free (AB271831)

  • 5
  • 1
Primary Antibodies

AB198933

Alexa Fluor® 488 Anti-Fibronectin antibody [F1]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Fibronectin antibody [F1] (AB198933)

  • 0
  • 1
Primary Antibodies

AB32419

Anti-Fibronectin antibody [F1]

20ul selling size|RabMAb|Recombinant|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1] (AB32419)

  • 5
  • 7
Primary Antibodies

AB219366

Anti-Fibronectin antibody [F1] - Low endotoxin, Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [F1] - Low endotoxin, Azide free (AB219366)

  • 4
  • 1

보완 제품

Primary Antibodies

AB138492

Anti-Collagen I antibody [EPR7785]

20ul selling size|BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Frozen sections) - Anti-Collagen I antibody [EPR7785] (AB138492)

  • 4
  • 37
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB7778

Anti-Collagen III antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Collagen III antibody (AB7778)

  • 4
  • 24
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.5 µg/mL", "sELISA-species-notes": "<p>For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [HFN 7.1] to Fibronectin - BSA and Azide free (<a href='/ko/products/unavailable/fibronectin-antibody-hfn-71-ab80923'>ab80923</a>) as Capture.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
Prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. This product has been sterile filtered.
보관 버퍼
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Add glycerol to a final volume of 50% for extra stability and aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (PubMed : 3024962, PubMed : 3593230, PubMed : 3900070, PubMed : 7989369). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation (PubMed : 34089617).. Anastellin. Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.. Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5 : ITGB1 integrin receptor signaling.
See full target information FN1
style
left-column

제품이 사용된 논문 (22)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer biology & medicine 22: PubMed39907104

2025

Carnitine palmitoyltransferase 1C promotes EMT-associated cisplatin resistance in non-small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Renjie Chen,Jiahui Wang,Shuoyu Huang,Xuefeng Hu,Xinran He,Tiange Zhang,Yunhan Hu,Huijun Wei,Sihui Nian,Yushu Huang,Zhihao Wu

BMC veterinary research 18:126 PubMed35366872

2022

NADH oxidase of Mycoplasma hyopneumoniae functions as a potential mediator of virulence.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Hao,Xing Xie,Zhixin Feng,Rong Chen,Yanna Wei,Jin Liu,Qiyan Xiong,Guoqing Shao,Johnson Lin

Frontiers in microbiology 12:747421 PubMed34671334

2021

Nicotinamide Adenine Dinucleotide-Dependent Flavin Oxidoreductase of Functions as a Potential Novel Virulence Factor and Not Only as a Metabolic Enzyme.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Xie,Fei Hao,Rong Chen,Jingjing Wang,Yanna Wei,Jin Liu,Haiyan Wang,Zhenzhen Zhang,Yun Bai,Guoqing Shao,Qiyan Xiong,Zhixin Feng

Journal of cancer research and clinical oncology : PubMed33471184

2021

Resveratrol induces PD-L1 expression through snail-driven activation of Wnt pathway in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Yang,Zongyu Li,Jianping Tao,Hao Hu,Zilin Li,Zhijian Zhang,Feng Cheng,Yihang Sun,Yao Zhang,Jianke Yang,Huijun Wei,Zhihao Wu

Methods in molecular biology (Clifton, N.J.) 2054:23-57 PubMed31482446

2019

Bioengineered Microtissue Models of the Human Bone Metastatic Microenvironment: A Novel In Vitro Theranostics Platform for Cancer Research.

Applications

Unspecified application

Species

Unspecified reactive species

Nathalie Bock

Journal of experimental & clinical cancer research 37:39 PubMed29482580

2018

Upregulation of lactate-inducible snail protein suppresses oncogene-mediated senescence through p16 inactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangrui Li,Zhijian Zhang,Yao Zhang,Yuxiang Cao,Huijun Wei,Zhihao Wu

Nature materials 16:953-961 PubMed28783156

2017

Hydrogels with precisely controlled integrin activation dictate vascular patterning and permeability.

Applications

Unspecified application

Species

Unspecified reactive species

Shuoran Li,Lina R Nih,Haylee Bachman,Peng Fei,Yilei Li,Eunwoo Nam,Robert Dimatteo,S Thomas Carmichael,Thomas H Barker,Tatiana Segura

PloS one 12:e0181151 PubMed28719644

2017

Isolation of putative stem cells present in human adult olfactory mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara Tanos,Alberto Maria Saibene,Carlotta Pipolo,Paolo Battaglia,Giovanni Felisati,Alicia Rubio

Cell death & disease 7:e2302 PubMed27441654

2016

Tankyrase inhibition aggravates kidney injury in the absence of CD2AP.

Applications

Unspecified application

Species

Unspecified reactive species

S Kuusela,H Wang,A A Wasik,H Suleiman,S Lehtonen

PloS one 11:e0147867 PubMed26828433

2016

The Salivary Scavenger and Agglutinin (SALSA) in Healthy and Complicated Pregnancy.

Applications

IHC

Species

Unspecified reactive species

Martin Parnov Reichhardt,Hanna Jarva,Anna Inkeri Lokki,Hannele Laivuori,Piia Vuorela,Vuokko Loimaranta,Andreas Glasner,Monika Siwetz,Berthold Huppertz,Seppo Meri
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com