Anti-FIF antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-FIF antibody (AB65836)

Immunocytochemistry/ Immunofluorescence of HeLa cells staining FIF using ab65836 at 1 μg/ml.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (AB65836)

This image shows human epidermal carcinoma tissue stained with ab65836 at 0.5-1µg/ml.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (AB65836)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human placenta tissue staining FIF using ab65836 at 1 μg/ml.

Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65836 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FIF antibody (AB65836)

ICC/IF image of ab65836 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65836, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (AB65836)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of rat intestine tissue staining FIF using ab65836 at 1 μg/ml.

Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65836 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (AB65836)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of Rat brain tissue staining FIF using ab65836 at 1 μg/ml.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIF antibody (AB65836)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse intestine tissue staining FIF using ab65836 at 1 μg/ml.

Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65836 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-FIF antibody (AB65836)

Immunohistochemistry (Frozen sections) of Rat cardiac muscel tissue staining FIF using ab65836 at 1 µg/ml.