AB224334

Anti-Filamin B antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal Filamin B antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FLNB aa 600 to C-terminus.

대체 명칭 보기

FLN1L, FLN3, TABP, TAP, FLNB, Filamin-B, FLN-B, ABP-278, ABP-280 homolog, Actin-binding-like protein, Beta-filamin, Filamin homolog 1, Filamin-3, Thyroid autoantigen, Truncated actin-binding protein, Fh1, Truncated ABP

11 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Paraffin-embedded human testis tissue stained for Filamin B using ab224334 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Filamin B antibody (AB224334)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Filamin B antibody (AB224334)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Filamin B (green) using ab224334 at 4 μg/ml in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Paraffin-embedded human kidney tissue stained for Filamin B using ab224334 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Paraffin-embedded human skin tissue stained for Filamin B using ab224334 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Paraffin-embedded human skeletal muscle tissue stained for Filamin B using ab224334 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Paraffin-embedded human pancreas tissue stained for Filamin B using ab224334 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Paraffin-embedded human duodenum tissue stained for Filamin B using ab224334 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Paraffin-embedded human prostate tissue stained for Filamin B using ab224334 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Paraffin-embedded human duodenum tissue stained for Filamin B using ab224334 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Filamin B antibody (AB224334)

All lanes:

Western blot - Anti-Filamin B antibody (ab224334) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II (Rat bladder carcinoma cell line) cell lysate

Predicted band size: 278 kDa

true

Unknown

Western blot - Anti-Filamin B antibody (AB224334)

All lanes:

Western blot - Anti-Filamin B antibody (ab224334) at 0.4 µg/mL

Lane 1:

A-549 cell line

Lane 2:

HEK 293 cell line

Predicted band size: 278 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human FLNB aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75369

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro.

See full target information FLNB

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

iScience 27:111487 PubMed39759007

2025

miR-450b-5p promotes development of endometriosis by inhibiting the GABPA/HOXD10 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Huang,Yidan Wang,Ruiyun Li,Yongmei Liu,Yuan Yang

Bone reports 20:101746 PubMed38463381

2024

Disruption of leads to skeletal malformation by interfering with skeletal segmentation through the gene.

Applications

Unspecified application

Species

Unspecified reactive species

Qiming Xu,Lijia Cui,Yude Lin,Leigh-Anne Cui,Weibo Xia

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Filamin B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Immunocytochemistry/ Immunofluorescence - Anti-Filamin B antibody (AB224334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filamin B antibody (AB224334)

Western blot - Anti-Filamin B antibody (AB224334)

Western blot - Anti-Filamin B antibody (AB224334)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise