AB72538

Anti-FIST antibody

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(6 제품이 사용된 논문 )

Rabbit Polyclonal FIST antibody. Suitable for WB, IHC-P and reacts with Mouse, Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human HIPK3.

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIST antibody (AB72538)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIST antibody (AB72538)

ab72538 at 10μg/ml staining FIST in human testis by Immunohistochemistry using paraffin-embedded tissue.

Unknown

Western blot - Anti-FIST antibody (AB72538)

All lanes:

Western blot - Anti-FIST antibody (ab72538) at 1 µg/mL

Lane 1:

Mouse testis lysate

Lane 2:

Mouse testis lysate with immunising peptide

Lane 3:

Rat testis lysate

Predicted band size: 134 kDa

Observed band size: 100 kDa,134 kDa,50 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human HIPK3. The exact immunogen used to generate this antibody is proprietary information.

Q9H422

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The FIST protein also known as Found in Squamous Cell Carcinoma Transporter possesses a mass of approximately 60 kDa. It functions mechanically as a transporter protein facilitating the movement of molecules across cellular membranes. Expression of FIST occurs in various tissues with particular abundance in squamous cell carcinoma cells where it plays a role in tumor cell survival and proliferation.

Biological function summary

FIST contributes to cellular processes by regulating the transport of specific substrates such as ions and small molecules critical for cellular homeostasis and energy balance. This protein is often part of a larger transporter complex allowing it to interact with other proteins to modulate its transport activities efficiently. Its role becomes more significant in squamous cell carcinoma where altered transport activities could contribute to the development and progression of the cancer.

Pathways

FIST has significant involvement in the cellular energy metabolism and signaling pathways. One key pathway is the ion transport pathway where FIST interacts with several channel proteins to maintain ionic concentration and cellular excitability. Moreover it associates with ATP-binding cassette (ABC) transporters participating in the regulation of multidrug resistance which provides cancer cells with a mechanism to survive chemotherapeutic treatments.

FIST has associations with squamous cell carcinoma and an increased risk of resistance to cancer therapies. Alterations in FIST expression or function can disrupt cellular ion homeostasis contributing to tumor development and progression. The protein links to the disease through its interaction with channel proteins affecting cell proliferation. Additionally its collaboration with ABC transporters in multidrug resistance pathways impacts the effectiveness of chemotherapy making the study of FIST critical in understanding and combating squamous cell carcinoma.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression. Phosphorylates JUN and RUNX2. Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. The phosphorylation of NR5A1 activates SF1 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. In osteoblasts, supports transcription activation : phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).

See full target information HIPK3

대체 명칭 보기

DYRK6, FIST3, PKY, HIPK3, Homeodomain-interacting protein kinase 3, Androgen receptor-interacting nuclear protein kinase, Fas-interacting serine/threonine-protein kinase, Homolog of protein kinase YAK1, ANPK, FIST

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

eLife 13: PubMed39041323

2024

circHIPK3 nucleates IGF2BP2 and functions as a competing endogenous RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Trine Line Hauge Okholm,Andreas Bjerregaard Kamstrup,Morten Muhlig Nielsen,Anne Kruse Hollensen,Mette Laugesen Graversgaard,Matilde Helbo Sørensen,Lasse Sommer Kristensen,Søren Vang,Samuel S Park,Eugene Yeo,Lars Dyrskjøt,Jørgen Kjems,Jakob Skou Pedersen,Christian Kroun Damgaard

iScience 27:108955 PubMed38322996

2024

Exosomal miR-3174 induced by hypoxia promotes angiogenesis and metastasis of hepatocellular carcinoma by inhibiting HIPK3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Yang,Mingyu Wu,Xiangxu Kong,Yun Wang,Chunyang Hu,Deming Zhu,Lianbao Kong,Fei Qiu,Wangjie Jiang

Bioscience reports 43: PubMed37581369

2023

MiR-21 attenuates FAS-mediated cardiomyocyte apoptosis by regulating HIPK3 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu Wang,Tingting Zhang,Jianlong Zhai,Zhongli Wang,Yan Wang,Lili He,Sai Ma,Hanying Xing,Yifang Guo

Aging 15:1025-1038 PubMed36800233

2023

Circ_0001052 promotes cardiac hypertrophy via elevating Hipk3.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyue Yang,Weichen Wang,Longlong Wang,Yuze Li

International journal of clinical and experimental 10:10603-10610 PubMed31966402

2017

ROR1 promotes the proliferation of endometrial cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Huilin Zhang,Xiaofang Yan,Jieqi Ke,Yongli Zhang,Chenyun Dai,Mei Zhu,Feizhou Jiang,Xiaoping Wan

British journal of cancer 109:452-61 PubMed23807165

2013

Dysregulation of miR-106a and miR-591 confers paclitaxel resistance to ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

J H Huh,T H Kim,K Kim,J-A Song,Y J Jung,J-Y Jeong,M J Lee,Y K Kim,D H Lee,H J An

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FIST antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FIST antibody (AB72538)

Western blot - Anti-FIST antibody (AB72538)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise