AB272337

FITC Anti-Bcl-2 antibody [Bcl-2/100]

Be the first to review this product! 리뷰 제출

(1 출판물)

Anti-Bcl-2 antibody [Bcl-2/100] conjugated to FITC - suitable for intracellular flow cytometry, and reacts with human samples. Clone [Bcl-2/100] is a mouse monoclonal antibody cited in 14 publications.

2 이미지

Flow Cytometry (Intracellular) - FITC Anti-Bcl-2 antibody [Bcl-2/100] (AB272337)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-Bcl-2 antibody [Bcl-2/100] (AB272337)

Flow cytometry intracellular staining pattern of human peripheral whole blood stained usingab272337 at 4 μl reagent / 100 μl of peripheral whole blood.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-Bcl-2 antibody [Bcl-2/100] (AB272337)

Separation of human Bcl-2 positive lymphocytes (red-filled) from blood debris (black-dashed) in Intracellular Flow Cytometry analysis (intracellular staining) of human peripheral whole blood stained using ab272337 at 4 μl reagent / 100 μl of peripheral whole blood.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

Bcl-2/100

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human BCL2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P10415

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p>The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10<sup>6</sup> cells in a suspension.</p>" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Size-exclusion chromatography

Purification 관련 사항

Purified from TCS.

보관 버퍼

pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Store in the dark

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Bcl-2 also known as B-cell lymphoma 2 is an important protein involved in the regulation of apoptosis. It mechanically functions by inhibiting cell death and is a member of the Bcl-2 family of proteins. Bcl-2 has a molecular weight of approximately 26 kDa. It is expressed mainly in fetal and adult tissues including the immune system and is notably present in hematopoietic stem cells and various cell lines derived from lymphoid tissues. In laboratory practices scientists often assess Bcl-2 expression levels through techniques like western blotting using antibodies such as 10c4 for detection.

Biological function summary

The role of Bcl-2 extends to maintaining cell survival and balancing apoptotic signals. It functions as an anti-apoptotic molecule within the cell primarily involved in forming complexes with pro-apoptotic members of the Bcl-2 family like Bax and Bak. These interactions prevent mitochondrial membrane permeabilization which is an important step in apoptotic signal transduction. As an important regulator of apoptosis Bcl-2 helps in cellular homeostasis and appropriate immune responses under physiological conditions.

Pathways

Bcl-2 plays a critical role in the intrinsic or mitochondrial pathway of apoptosis. Within this pathway Bcl-2 interacts with other proteins such as cytochrome c and APAF-1 to inhibit apoptosis. This pathway involves a network of Bcl-2 family proteins where Bcl-2's anti-apoptotic role counteracts the pro-apoptotic activity of proteins like Bax. These interactions help fine-tune the apoptotic response to various internal stimuli maintaining cellular integrity and function.

The dysregulation of Bcl-2 expression has been implicated in cancer and autoimmune diseases. Overexpression of Bcl-2 is associated with several types of cancer including lymphomas and breast cancer where its anti-apoptotic property leads to tumor cell survival and resistance to chemotherapy. Bcl-2 also connects to disorders such as autoimmune diseases due to its regulation of cell survival with proteins like Bim playing an opposite role in promoting apoptosis. Understanding Bcl-2's functionality within these contexts is essential for developing targeted therapies.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed : 1508712, PubMed : 8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed : 11368354). Appears to function in a feedback loop system with caspases (PubMed : 11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed : 11368354). Also acts as an inhibitor of autophagy : interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed : 18570871, PubMed : 20889974, PubMed : 21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed : 17418785).

See full target information BCL2

대체 명칭 보기

Apoptosis regulator Bcl-2, BCL2

style

left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 26:203-219 PubMed36450891

2022

Comprehensive transcriptomic profiling and mutational landscape of primary gastric linitis plastica.

Applications

Unspecified application

Species

Unspecified reactive species

Zhu Liu,Lian-Lian Hong,Jin-Sen Zheng,Zhe-Nan Ling,Zhi-Long Zhang,Ya-Nan Qi,Xin-Yu Zhang,Tian-Yu Zhu,Jiu-Li Wang,Jing Han,Xiang-Liu Chen,Qi-Ming Yu,Shi Wang,Pei Li,Zhi-Qiang Ling

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Bcl-2 antibody [Bcl-2/100]

Flow Cytometry (Intracellular) - FITC Anti-Bcl-2 antibody [Bcl-2/100] (AB272337)

Flow Cytometry (Intracellular) - FITC Anti-Bcl-2 antibody [Bcl-2/100] (AB272337)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise