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AB34444

FITC Anti-CD11b antibody [2F4/11]

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Anti-CD11b antibody [2F4/11] conjugated to FITC - suitable for flow cytometry, and reacts with pig samples. Clone [2F4/11] is a mouse monoclonal antibody cited in 11 publications.

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Flow Cytometry - FITC Anti-CD11b antibody [2F4/11] (AB34444)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD11b antibody [2F4/11] (AB34444)

Flow cytometry staining of red cell lysed porcine blood with ab34444.

Figure A. APC conjugated mouse anti porcine CD31 and FITC conjugated mouse IgG1 isotype control. Figure B. APC conjugated mouse anti porcine CD31 and FITC conjugated mouse anti porcine CD11R3 (ab34444).

All experiments performed on red cell lysed porcine blood gated on lymphocytes (10ul neat to label 1x106 cells in 100ul).

Data acquired on the ZE5 Cell Analyzer.

Flow Cytometry - FITC Anti-CD11b antibody [2F4/11] (AB34444)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD11b antibody [2F4/11] (AB34444)

Flow cytometry staining of red cell lysed porcine blood with ab34444.

Figure A. APC conjugated mouse anti porcine CD31 and FITC conjugated mouse IgG1 isotype control. Figure B. APC conjugated mouse anti porcine CD31 and FITC conjugated mouse anti porcine CD11R3 (ab34444).

All experiments performed on red cell lysed porcine blood gated on mononuclear cells (10ul neat to label 1x106 cells in 100ul) .

Flow Cytometry - FITC Anti-CD11b antibody [2F4/11] (AB34444)
  • Flow Cyt

Supplier Data

Flow Cytometry - FITC Anti-CD11b antibody [2F4/11] (AB34444)

Flow cytometry staining of red cell lysed porcine blood with ab34444.

Figure A. FITC conjugated mouse anti porcine CD11R3 (ab34444) and APC conjugated mouse IgG1 isotype control. Figure B. FITC conjugated mouse anti porcine CD11R3 (ab34444) and APC conjugated mouse anti porcine CD31.

All experiments performed on red cell lysed porcine blood gated on mononuclear cells (10ul neat to label 1x106 cells in 100ul).

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

2F4/11

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Pig

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>ab34444 is expressed on granulocytes, monocytes and alveolar macrophages, but not on lymphocytes, eythrocytes or platelets.</p>

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대체품

Primary Antibodies

AB184308

Anti-CD11b antibody [EPR19387]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EPR19387] (AB184308)

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Primary Antibodies

AB133357

Anti-CD11b antibody [EPR1344]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD11b antibody [EPR1344] (AB133357)

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  • 37
Primary Antibodies

AB52478

Anti-CD11b antibody [EP1345Y] - C-terminal

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (AB52478)

  • 5
  • 5
Primary Antibodies

AB187537

Anti-CD11b antibody [EP1345Y] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - BSA and Azide free (AB187537)

  • 4
  • 1
Primary Antibodies

AB216445

Anti-CD11b antibody [EPR1344] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EPR1344] - Low endotoxin, Azide free (AB216445)

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보완 제품

Primary Antibodies

AB58754

PE Anti-CD44 antibody [MEM-263]

Flow Cytometry - PE Anti-CD44 antibody [MEM-263] (AB58754)

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Primary Antibodies

AB314289

Alexa Fluor® 647 Anti-CD19 antibody [EPR23174-145]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD19 antibody [EPR23174-145] (AB314289)

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  • 0
Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

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Primary Antibodies

AB302698

Alexa Fluor® 647 Anti-CD14 antibody [SP192]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD14 antibody [SP192] (AB302698)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Pig": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Neat - 1/10. Use 10ul of the suggested working dilution to 1x10^6 cells in 100ul. <a href='/ko/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }
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제품 세부 정보

ab34444 is reported to block phagocytosis of complement-opsonised zymosan particles by polymononuclear granulocytes and alveolar macrophages.
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
보관 버퍼
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Store in the dark
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

See full target information CD11b

대체 명칭 보기

Antigen CD11b p170, CD11 antigen-like family member B, CD11b/CD18, CD49D, CR 3 alpha chain (CR3A), CR-3 alpha chain, CR3, CR3A, Cell surface glycoprotein MAC-1 subunit alpha, Complement Component Receptor 3 Alpha, Complement component 3 receptor 3 subunit, Complement receptor type 3, Complement receptor type 3, alpha subunit, F730045J24Rik, ITAM_HUMAN, ITGAM, Integrin alpha M chain, Integrin alpha-M, Integrin beta 2 alpha subunit, Integrin subunit alpha M, Leukocyte adhesion receptor MO1, Ly-40, MAC 1, MGC117044, MO1A, Mac-1a, Mac1, alpha subunit, Macrophage antigen alpha polypeptide, Mo1, Mo1, alpha subunit, Neutrophil adherence receptor, Neutrophil adherence receptor alpha M subunit, SLEB6, antigen CD11b (p170), integrin, alpha M (complement component 3 receptor 3 subunit)
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in plant science 11:440 PubMed32328082

2020

Removal of N-Terminal Fusion Domains From Recombinant Target Proteins Produced in .

Applications

Unspecified application

Species

Unspecified reactive species

Md Reyazul Islam,Seoyoung Choi,Thangarasu Muthamilselvan,Kunyoo Shin,Inhwan Hwang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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