AB210230

FITC Anti-FOXP3 antibody [3G3]

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(6 제품이 사용된 논문 )

Anti-FOXP3 antibody [3G3] conjugated to FITC - suitable for intracellular flow cytometry, and reacts with mouse samples. Clone [3G3] is a mouse monoclonal antibody cited in 74 publications.

1 이미지

Flow Cytometry (Intracellular) - FITC Anti-FOXP3 antibody [3G3] (AB210230)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - FITC Anti-FOXP3 antibody [3G3] (AB210230)

Intracellular flow cytometric analysis of Mouse C57Bl/6 splenocytes stained with APC Anti-Mouse CD4, followed by intracellular labeling of Foxp3 with ab210230 at 0.015 ug/test (right panel) or FITC Mouse IgG1 isotype control at 0.015 ug/test (left panel).

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

3G3

Isotype

IgG1

Light chain 타입

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt (Intra)

applications

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left-column

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rhesus monkey": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

ab210230 was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation

보관 버퍼

pH: 8 Preservative: 0.05% Sodium azide Constituents: 0.87% Sodium chloride, 0.12% Tris, 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze|Store in the dark

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left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FOXP3 also recognized as Forkhead box P3 is a transcription factor critical for immune system function. It weighs approximately 47 kDa. FOXP3 is mainly expressed in regulatory T cells (Tregs) which play a role in maintaining immune tolerance. Scientists primarily identify its presence in the nucleus where it facilitates transcriptional regulation. Methods like 'FOXP3 IHC' and 'FOXP3 IHC mouse' are useful for detecting this protein in tissue samples while 'FOXP3 antibodies' aid in research on this target. Techniques like 'FOXP3 staining' and 'FOXP3 flow cytometry' further allow for the analysis of its expression and function.

Biological function summary

This transcription factor operates by regulating the expression of a variety of genes involved in Treg cell development and function. FOXP3 can interact with other proteins to form complexes that modulate gene expression. Its role is essential in enabling Tregs to suppress immune responses a process necessary for preventing autoimmunity. The interaction extends to other key regulators within Tregs such as the protein complex 221D which may have overlapping functional responsibilities. 'FOXP3 236a/E7' reflects its genetic variants detectable in different studies providing insights into its biological functions.

Pathways

FOXP3 is a significant player within the immune regulation pathway. It integrates into the TGF-beta signaling pathway in which it controls the expression of genes important for Treg functions. The protein recruits other transcription factors like NF-kB coordinating intricate network regulations within immune cells. FOXP3's activity orchestrates the balance between immune activation and suppression ensuring a well-functioning immune response that prevents overreactions leading to autoimmune conditions.

FOXP3 mutations or dysregulation can link to autoimmune diseases like immune dysregulation polyendocrinopathy enteropathy X-linked syndrome (IPEX). This transcription factor's malfunction implicates cancer where inadequate Treg function can either suppress antitumor immunity or result in tumor progression. Proteins such as CTLA-4 which is also involved in immune regulation connect to FOXP3 in these contexts emphasizing FOXP3’s significant role in maintaining immune homeostasis and its impact on disease development.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg) (PubMed : 17377532, PubMed : 21458306, PubMed : 23947341, PubMed : 24354325, PubMed : 24722479, PubMed : 24835996, PubMed : 30513302, PubMed : 32644293). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells (PubMed : 23169781). Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases (PubMed : 17377532, PubMed : 21458306, PubMed : 23947341, PubMed : 24354325, PubMed : 24722479). The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG) (PubMed : 17377532, PubMed : 21458306, PubMed : 23947341, PubMed : 24354325, PubMed : 24722479). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed : 15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed : 17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed : 17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed : 18368049). Inhibits the transcriptional activator activity of RORA (PubMed : 18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (By similarity).

See full target information FOXP3

대체 명칭 보기

IPEX, JM2, FOXP3, Forkhead box protein P3, Scurfin

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell biology and toxicology 41:82 PubMed40335763

2025

ELF4 improves sepsis-induced myocardial injury by regulating STING signaling-mediated T cells differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yawen Zheng,Xiongjun Peng,Yusha Zhang,Ruilin Liu,Junke Long

Journal of cellular and molecular medicine 29:e70336 PubMed39993960

2025

Discovery of Endothelial-Monocyte Crosstalk in Ischemic-Reperfusion Injury Following Liver Transplantation Based on Integration of Single-Cell RNA and Transcriptome RNA Sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Sun,Li Li,Dan Li,Zhengxin Wang

Journal of biochemical and molecular toxicology 38:e23842 PubMed39588744

2024

Role of PD-1 in modulating IFN-γ-CXCL9/10-CXCR3 signaling in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Hong,Fang Huang,Zexian Hu,Qian Dong,Yan Kong,Xuan Zheng,Man Li,Yanzhi Cui

International immunopharmacology 138:112546 PubMed38917522

2024

MC1R regulates T regulatory cell differentiation through metabolic reprogramming to promote colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoliang Zhu,Mengjie Zou,Chunxing Li,Yuntian Tang,Honglin Luo,Xiaofeng Dong

Nephron 146:528-538 PubMed35176745

2022

Losartan Attenuates Atherosclerosis in Uremic Mice by Regulating Treg/Th17 Balance via Mediating PTEN/PI3K/Akt Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shanzhai Wei,Jie Sun,Yibei Li,Kangchun Xu,Man Wang,Yilai Zhang

International archives of allergy and immunology 183:375-388 PubMed34942624

2021

Mechanism of miR-181a-5p in Regulatory T/T-Helper 17 Immune Imbalance and Asthma Development in Mice with Allergic Rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoyun He,Yujuan Chen,Xiaoer Chen,Binfan Yuan

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-FOXP3 antibody [3G3]

Flow Cytometry (Intracellular) - FITC Anti-FOXP3 antibody [3G3] (AB210230)

관련 conjugated 항체와 다양한 조성의 항체 (6)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise