AB69545

FITC Anti-Heme Oxygenase 1 antibody [HO-1-2]

Name: FITC Anti-Heme Oxygenase 1 antibody [HO-1-2]
Brand: Abcam Limited
SKU: AB69545
Rating: 4 (1 reviews)

(1 리뷰)

(6 제품이 사용된 논문 )

Anti-Heme Oxygenase 1 antibody [HO-1-2] conjugated to FITC - suitable for intracellular flow cytometry, and reacts with human samples. Clone [HO-1-2] is a mouse monoclonal antibody cited in 12 publications.

1 이미지

Flow Cytometry (Intracellular) - FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (AB69545)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (AB69545)

Flow Cytometry of 10⁶ Jurkat cells stained using ab69545 at a concentration of 100 µg/mL.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

HO-1-2

Isotype

IgG2b

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Native Full Length Protein corresponding to Rat Hmox1.

P06762

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/fitc-mouse-igg2b-plpv219-isotype-control-ab91368'>ab91368</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }

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제품 세부 정보

This product was changed from ascites to tissue culture supernatant on 14^th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purified from TCS

보관 버퍼

Preservative: 0.09% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Heme Oxygenase 1 also known as HO-1 or HMOX1 is an enzyme that plays an important mechanistic role in heme catabolism. It catalyzes the degradation of heme into biliverdin carbon monoxide and free iron. This process involves the cleavage of the heme ring. HO-1 has a molecular weight of approximately 32 kDa. It is widely expressed in numerous tissues but is especially abundant in the liver and spleen. Its expression is induced by heme and other stress stimuli such as heavy metals cytokines and reactive oxygen species.

Biological function summary

Heme Oxygenase 1 serves important protective functions in the body. It is not part of a larger complex but its products such as carbon monoxide and biliverdin have their own biological activities. Carbon monoxide produced by HO-1 has antiflammatory properties and can modulate apoptotic pathways. Biliverdin is reduced to bilirubin which acts as an antioxidant. The enzyme therefore directly influences cellular stress responses and maintains cellular homeostasis through these processes.

Pathways

Heme Oxygenase 1 is integrally involved in oxidative stress response and heme metabolism. It participates in the cellular response to oxidative damage by reducing oxidative stress and promoting cytoprotection. Through its heme degradation activity it is connected with the synthesis of biologically active molecules like bilirubin and carbon monoxide. Heme Oxygenase 1 activity is related to other proteins in oxidative stress pathways such as Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) which regulates its expression and globins which are sources of heme for HO-1 activity.

Heme Oxygenase 1 has been linked to conditions like cardiovascular diseases and neurodegenerative disorders. Its expression can attenuate the severity of atherosclerosis where oxidative stress is an important factor. In neurodegenerative diseases HO-1’s antioxidant properties may provide neuroprotection by mitigating oxidative damage. The protein's interactions with inflammatory cytokines such as Interleukin-6 and tumor necrosis factor-alpha influence its activity in these disease contexts.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed : 11121422, PubMed : 19556236, PubMed : 7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed : 20055707).. Heme oxygenase 1. (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron.

See full target information HMOX1

대체 명칭 보기

HO, HO1, HMOX1, Heme oxygenase 1, HO-1

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

eLife 10: PubMed34762046

2021

The mitochondrial iron transporter ABCB7 is required for B cell development, proliferation, and class switch recombination in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Jonathan Lehrke,Michael Jeremy Shapiro,Matthew J Rajcula,Madeleine M Kennedy,Shaylene A McCue,Kay L Medina,Virginia Smith Shapiro

Frontiers in pharmacology 11:662 PubMed32508636

2020

Dihydroquercetin Activates AMPK/Nrf2/HO-1 Signaling in Macrophages and Attenuates Inflammation in LPS-Induced Endotoxemic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Lei,Yunfei Chai,Haoming Lin,Chunbo Chen,Mingyi Zhao,Weiping Xiong,Jian Zhuang,Xiaoping Fan

The Kaohsiung journal of medical sciences 36:741-749 PubMed32311231

2020

MiR-370 accelerated cerebral ischemia reperfusion injury via targeting SIRT6 and regulating Nrf2/ARE signal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong-Fan Ruan,Ming Xie,Shu-Jia Gui,Fang Lan,Juan Wan,Yan Li

Journal of immunology (Baltimore, Md. : 1950) 190:6378-88 PubMed23650615

2013

Inhaled carbon monoxide accelerates resolution of inflammation via unique proresolving mediator-heme oxygenase-1 circuits.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Chiang,Masakazu Shinohara,Jesmond Dalli,Valbona Mirakaj,Megumi Kibi,Augustine M K Choi,Charles N Serhan

PLoS pathogens 8:e1002579 PubMed22438807

2012

HMOX1 gene promoter alleles and high HO-1 levels are associated with severe malaria in Gambian children.

Applications

Flow Cyt

Species

Human

Michael Walther,Adam De Caul,Peter Aka,Madi Njie,Alfred Amambua-Ngwa,Brigitte Walther,Irene M Predazzi,Aubrey Cunnington,Susanne Deininger,Ebako N Takem,Augustine Ebonyi,Sebastian Weis,Robert Walton,Sarah Rowland-Jones,Giorgio Sirugo,Scott M Williams,David J Conway

Blood 117:4826-35 PubMed21389316

2011

The impact of inflammatory licensing on heme oxygenase-1-mediated induction of regulatory T cells by human mesenchymal stem cells.

Applications

Flow Cyt

Species

Human

Dimitrios Mougiakakos,Regina Jitschin,C Christian Johansson,Riki Okita,Rolf Kiessling,Katarina Le Blanc

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-Heme Oxygenase 1 antibody [HO-1-2]

Flow Cytometry (Intracellular) - FITC Anti-Heme Oxygenase 1 antibody [HO-1-2] (AB69545)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise