AB221

FITC Anti-Integrin alpha 4/CD49D antibody [44H6]

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(2 제품이 사용된 논문 )

Anti-Integrin alpha 4/CD49D antibody [44H6] conjugated to FITC - suitable for flow cytometry, and reacts with human samples. Clone [44H6] is a mouse monoclonal antibody cited in 13 publications.

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Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [44H6] (AB221)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [44H6] (AB221)

ab221 staining Integrin alpha 4/CD49D (FITC) in human peripheral blood lymphocytes by Flow Cytometry analysis.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

44H6

Isotype

IgG1

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

44H6 recognises the alpha chain of VLA 4. It reacts with ALL and AML cells, T-cells, B-cells and monocytes. No reactivity is observed in human kidney or brain sections. 44H6 will induce homotypic aggregation.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>or 100μl whole blood.</p><p><a href='/ko/products/primary-antibodies/fitc-mouse-igg1-b11-6-isotype-control-ab91356'>ab91356</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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제품 세부 정보

Purified IgG conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.6 : 1.0.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

보관 버퍼

Preservative: 0.1% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 4 also known as CD49D or ITGA4 is a transmembrane receptor with a mass of approximately 150 kDa. This protein is expressed on the surface of leukocytes including T and B lymphocytes monocytes and eosinophils. Integrin alpha 4 functions by interacting mechanically with the extracellular matrix components and endothelial cell counter-receptors like VCAM-1 facilitating cell adhesion and migration. Its expression is vital for the immune cells' ability to move and locate sites of inflammation or injury.

Biological function summary

Integrin alpha 4 forms a heterodimer complex with beta subunits like beta 1 (forming VLA-4) or beta 7 allowing it to mediate adhesion and signaling pathways. In particular the alpha 4 and beta 1 dimerization gives rise to a high-affinity receptor for fibronectin and VCAM-1. This interaction is essential for regulating immune responses and maintaining cell positioning within tissues. The presence of this complex on leukocytes drives their ability to navigate through the vasculature to inflamed areas.

Pathways

Integrin alpha 4 participates in significant signal transduction pathways including the MAPK and PI3K-Akt pathways. These pathways modulate cell survival proliferation and migration. In the MAPK pathway integrin alpha 4 influences cell signaling cascades by interacting with other proteins like TALIN or PAXILLIN which are important for integrin-mediated signal relay. In connection with PI3K-Akt pathway alpha 4 integrins work closely with proteins in regulating cellular adhesion and motility showing a dynamic interplay in immune regulation.

Integrin alpha 4 plays an important role in various immune-related diseases such as multiple sclerosis and Crohn's disease. The protein's participation in leukocyte adhesion and trafficking makes it a target for therapeutic intervention. Natalizumab a monoclonal antibody against alpha 4 integrin showcases the critical link between the protein and disease treatment by blocking leukocyte migration to reduce inflammation. Additionally integrin alpha 4's interaction with proteins like VCAM-1 and VLA-4 highlights its relevance in these autoimmune conditions highlighting its importance in disease pathophysiology.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4 : ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed : 23125415). ITGA4 : ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed : 18635536, PubMed : 25398877). Integrin ITGA4 : ITGB1 represses PRKCA-mediated L-type voltage-gated channel Ca(2+) influx and ROCK-mediated calcium sensitivity in vascular smooth muscle cells via its interaction with SVEP1, thereby inhibiting vasocontraction (PubMed : 35802072).

See full target information ITGA4

대체 명칭 보기

CD49d, CD49D, ITGA4, Integrin alpha-4, CD49 antigen-like family member D, Integrin alpha-IV, VLA-4 subunit alpha

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in cellular neuroscience 13:523 PubMed31849612

2019

BMP2 Is Related to Hirschsprung's Disease and Required for Enteric Nervous System Development.

Applications

Unspecified application

Species

Unspecified reactive species

Sizhou Huang,Yi Wang,Lingfei Luo,Xiaoqing Li,Xianqing Jin,Shuangshuang Li,Xiaoping Yu,Min Yang,Zhenhua Guo

Bone research 6:34 PubMed30510839

2018

RANKL signaling in bone marrow mesenchymal stem cells negatively regulates osteoblastic bone formation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Chen,Xin Zhi,Jun Wang,Jiacan Su

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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FITC Anti-Integrin alpha 4/CD49D antibody [44H6]

Flow Cytometry - FITC Anti-Integrin alpha 4/CD49D antibody [44H6] (AB221)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise