AB95648

FITC Anti-TCR alpha + TCR beta antibody [IP26]

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Anti-TCR alpha + TCR beta antibody [IP26] conjugated to FITC - suitable for flow cytometry, and reacts with human samples. Clone [IP26] is a mouse monoclonal antibody and one of the most commonly used clones for flow cytometry.

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Flow Cytometry - FITC Anti-TCR alpha + TCR beta antibody [IP26] (AB95648)

Flow Cyt

Unknown

Flow Cytometry - FITC Anti-TCR alpha + TCR beta antibody [IP26] (AB95648)

Normal human peripheral blood cells were stained with Mouse IgG1 - Isotype Control FITC (open histogram) or ab95648 (filled histogram). Cells in the lymphocyte gate were used for analysis.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

IP26

Isotype

IgG1

Light chain 타입

kappa

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Full Length Protein corresponding to Human TRBV7-9. The exact immunogen used to generate this antibody is proprietary information.

P04435

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/ko/products/primary-antibodies/fitc-mouse-igg1-kappa-mopc-21-isotype-control-ab106163'>ab106163</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.87% Sodium chloride, 0.2% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The T-cell receptor (TCR) alpha and TCR beta sometimes referred to as alpha beta TCR or alfa TCR are essential components of the immune response machinery. TCR alpha and beta chains each have a mass that varies between 30-40 kDa depending on the variable region's length. These receptor chains are expressed on the surface of T-cells more specifically on the vast majority of T lymphocytes and play a critical role in antigen recognition. Commercial names like anti-TCR or H57 might refer to antibodies targeting these chains.

Biological function summary

TCR alpha and beta chains form a heterodimeric complex with other important proteins like CD3 to transmit signals inside the T-cell upon antigen binding. This complex becomes active when T-cells recognize peptide antigens presented by major histocompatibility complex (MHC) molecules on antigen-presenting cells. The interaction between TCR and MHC-peptide complex initializes T-cell activation and subsequent immune responses being central to adaptive immunity.

Pathways

TCR alpha and beta are integral to the T-cell receptor signaling pathway. This pathway facilitates the activation of important transcription factors such as NF-kB and AP-1 which drive T-cell proliferation and differentiation. The signaling involves several other proteins such as protein kinase C theta and ZAP-70 which further propagate intracellular signaling cascades essential for a fully functional immune response.

Mutations or malfunctions in TCR alpha and beta chains can associate with autoimmune disorders like rheumatoid arthritis and certain types of immunodeficiency. Rheumatoid arthritis involves aberrant TCR signaling pathways that result in inappropriate immune responses against self-antigens. In some immunodeficiencies defective TCR signaling impairs T-cell development and function potentially involving related proteins like CD3 and ZAP-70 leading to compromised immune defenses.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Constant region of T cell receptor (TR) alpha chain (PubMed : 24600447). Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens (PubMed : 25493333). Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation (PubMed : 23524462). The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity (PubMed : 15040585).

See full target information TRAC

대체 명칭 보기

TCRA, TRAC, T cell receptor alpha chain constant, TCRBC2, TRBC2, T cell receptor beta constant 2, TCR beta

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of immunology (Baltimore, Md. : 1950) 172:4091-9 PubMed15034021

2004

Src homology 2 domain-containing protein tyrosine phosphatase substrate 1 regulates the migration of Langerhans cells from the epidermis to draining lymph nodes.

Applications

Unspecified application

Species

Unspecified reactive species

Atsushi Fukunaga,Hiroshi Nagai,Tetsuya Noguchi,Hideki Okazawa,Takashi Matozaki,Xijun Yu,Carl F Lagenaur,Nakayuki Honma,Masamitsu Ichihashi,Masato Kasuga,Chikako Nishigori,Tatsuya Horikawa

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

FITC Anti-TCR alpha + TCR beta antibody [IP26]

Flow Cytometry - FITC Anti-TCR alpha + TCR beta antibody [IP26] (AB95648)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise