Anti-FLI1 antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-FLI1 antibody (AB153909)

Immunofluorescennt analysis of paraformaldehyde-fixed HeLa cells (4%, 15 mins) labeling FLI1 with ab153909 at 1/500 dilution (Green). Right image shows cells co-stained with Hoechst 33342 (Blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody (AB153909)

Immunohistochemical analysis of paraffin-embedded Human SkHep1 xenograft tissue labeling FLI1 with ab153909 at 1/500 dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-FLI1 antibody (AB153909)

FLI1 antibody immunoprecipitates FLI1 protein in IP experiments. Samples were separated by 10 % SDS-PAGE.
The immunoprecipitated FLI1 protein was detected by FLI1 antibody diluted at 1/500 dilution.

All lanes:

Immunoprecipitation - Anti-FLI1 antibody (ab153909) at 4 µg

Lane 1:

Raji whole cell extract at 40 µg

Lane 2:

Control with 4 µg of preimmune Rabbit IgG

Lane 3:

Immunoprecipitated Raji whole cell extract using ab153909

Secondary

All lanes:

Anti-Rabbit IgG

Predicted band size: 51 kDa

false

• WB

Unknown

Western blot - Anti-FLI1 antibody (AB153909)

10% SDS PAGE

All lanes:

Western blot - Anti-FLI1 antibody (ab153909) at 1/1000 dilution

Lane 1:

Raji whole cell lysate at 30 µg

Lane 2:

THP1 whole cell lysate at 30 µg

Predicted band size: 51 kDa

false

• WB

Supplier Data

Western blot - Anti-FLI1 antibody (AB153909)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-FLI1 antibody (ab153909) at 1/1000 dilution

All lanes:

Rat kidney lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 51 kDa

false