JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB124791

Anti-FLI1 antibody [EPR4645]

5

(1 리뷰)

|

(5 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal FLI1 antibody. Suitable for WB and reacts with Recombinant fragment, Mouse, Rat, Human samples. Cited in 5 publications.
2 이미지
Western blot - Anti-FLI1 antibody [EPR4645] (AB124791)
  • WB

Unknown

Western blot - Anti-FLI1 antibody [EPR4645] (AB124791)

All lanes:

Western blot - Anti-FLI1 antibody [EPR4645] (ab124791) at 1/1000 dilution

Lane 1:

Raw264.7 cell lysate at 10 µg

Lane 2:

U937 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

K562 cell lysate at 10 µg

Lane 5:

C6 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 51 kDa

false

Western blot - Anti-FLI1 antibody [EPR4645] (AB124791)
  • WB

Unknown

Western blot - Anti-FLI1 antibody [EPR4645] (AB124791)

All lanes:

Western blot - Anti-FLI1 antibody [EPR4645] (ab124791) at 1/1000 dilution

Lane 1:

ERG recombinant protein at 0.01 µg

Lane 2:

FLI1 recombinant protein at 0.01 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 51 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-FLI1 antibody [EPR4645] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4645

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This product does not cross-react with ERG.

style
left-column

대체품

Primary Antibodies

AB153909

Anti-FLI1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-FLI1 antibody (AB153909)

  • 0
  • 0
Primary Antibodies

AB218865

Anti-FLI1 antibody [FLI1/1312] - BSA and Azide free

Western blot - Anti-FLI1 antibody [FLI1/1312] - BSA and Azide free (AB218865)

  • 0
  • 0
Primary Antibodies

AB180902

Anti-FLI1 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-FLI1 antibody - C-terminal (AB180902)

  • 0
  • 0
Primary Antibodies

AB133485

Anti-FLI1 antibody [EPR4646]

20ul selling size|RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] (AB133485)

  • 5
  • 1
Primary Antibodies

AB215987

Anti-FLI1 antibody [EPR4646] - BSA and Azide free

RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FLI1 antibody [EPR4646] - BSA and Azide free (AB215987)

  • 0
  • 0

보완 제품

Primary Antibodies

AB133288

Anti-EWSR1/EWS antibody [EPR4647]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-EWSR1/EWS antibody [EPR4647] (AB133288)

  • 0
  • 0
Primary Antibodies

AB92513

Anti-ERG antibody [EPR3864]

BOND RX™ Validated|RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERG antibody [EPR3864] (AB92513)

  • 5
  • 18
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB172730

Rabbit IgG, monoclonal [EPR25A] - Isotype Control

RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (AB172730)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Recombinant fragment": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The FLI1 protein also known as Friend leukemia virus integration 1 acts as a transcription factor. It consists of 452 amino acids resulting in a molecular mass of about 50 kDa. FLI1 is part of the ETS (E26 transformation-specific) family of transcription factors and it binds to specific DNA sequences to regulate the expression of various genes. It expresses in hematopoietic cells endothelial cells and several tissues during development. High expression levels in certain tissues suggest a significant role in diverse cellular functions.
Biological function summary

The FLI1 protein influences cell proliferation differentiation and apoptosis. It also plays a role in the regulation of genes involved in the immune response. FLI1 forms complexes with other proteins to exert its function therefore impacting gene expression in cellular contexts. Its participation in these processes indicates its regulatory impact on normal cellular development and function.

Pathways

FLI1 interacts with signaling networks that govern the hematopoietic lineage and endothelial cell function. It participates in the MAPK/ERK pathway influencing cell cycle and growth signaling. Through these pathways FLI1 has functional interactions with proteins such as GATA1 and RUNX1 establishing its importance in vascular development and hematopoiesis.

FLI1 has significant associations with Ewing sarcoma and certain leukemias. In Ewing sarcoma chromosomal translocations involve FLI1 leading to oncogenic fusion proteins that drive tumorigenesis. It also interacts with partners like EWSR1 in this context. Moreover abnormal levels of FLI1 relate to blood disorders due to its role in hematopoietic regulation. Understanding these interactions and associations aids in developing therapeutic strategies targeting these illnesses.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Sequence-specific transcriptional activator (PubMed : 24100448, PubMed : 26316623, PubMed : 28255014). Recognizes the DNA sequence 5'-C[CA]GGAAGT-3'.
See full target information FLI1

대체 명칭 보기

Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB, FLI1
style
left-column

제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록and refine your search

Haematologica 110:2792-2798 PubMed40468949

2025

Characterization of a novel mutation in a family with thrombocytopenia and other congenital malformations.

Applications

Unspecified application

Species

Unspecified reactive species

Daniele Ammeti,Serena Barozzi,Alessandro Pecci,Melania Eva Zanchetta,Edward Cesnik,Alessandra Ferlini,Mariabeatrice Sanchini,Laura Verga,Valeria Bozzi,Fabio Corsolini,Michela Faleschini,Anna Savoia,Stefania Bigoni

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 74:28 PubMed39862276

2025

Pericytes mediate neuroinflammation via Fli-1 in endotoxemia and sepsis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Pengfei Li,Liu Liu,Perry V Halushka,Maria Trojanowska,Guirong Wang,Adviye Ergul,Hongkuan Fan

International journal of molecular sciences 25: PubMed39201581

2024

Antibody to Endogenous Cardiotonic Steroid Reverses Vascular Fibrosis and Restores Vasorelaxation in Chronic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia I Agalakova,Elena V Mikhailova,Ivan A Ershov,Olga V Nadei,Arseny A Pyankov,Michael M Galagoudza,C David Adair,Irina V Romanova,Alexei Y Bagrov

Kidney diseases (Basel, Switzerland) 8:424-435 PubMed36466072

2022

SENP3 Aggravates Renal Tubular Epithelial Cell Apoptosis in Lipopolysaccharide-Induced Acute Kidney Injury via deSUMOylation of Drp1.

Applications

Unspecified application

Species

Unspecified reactive species

Lirui Wang,Jiangtao Li,Chen Yu

Nature chemical biology 16:50-59 PubMed31819276

2019

CPSF3-dependent pre-mRNA processing as a druggable node in AML and Ewing's sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan T Ross,Felix Lohmann,Seth Carbonneau,Aleem Fazal,Wilhelm A Weihofen,Scott Gleim,Michael Salcius,Frederic Sigoillot,Martin Henault,Sarah H Carl,Juan B Rodríguez-Molina,Howard R Miller,Scott M Brittain,Jason Murphy,Mark Zambrowski,Geoffrey Boynton,Yuan Wang,Aye Chen,Gregory J Molind,Johannes H Wilbertz,Caroline G Artus-Revel,Min Jia,Favour A Akinjiyan,Jonathan Turner,Judith Knehr,Walter Carbone,Sven Schuierer,John S Reece-Hoyes,Kevin Xie,Chitra Saran,Eric T Williams,Guglielmo Roma,Matt Spencer,Jeremy Jenkins,Elizabeth L George,Jason R Thomas,Gregory Michaud,Markus Schirle,John Tallarico,Lori A Passmore,Jeffrey A Chao,Rohan E J Beckwith
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com