Anti-FLJ11506 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-FLJ11506 antibody (AB241294)

FLJ11506 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241294 used for IP at 50 μl per reaction. For WB at a 1/400 dilution.

Lane 1 : ab241294 IP in Jurkat whole cell lysate.
Lane 2 : Control IgG.
Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-FLJ11506 antibody (ab241294)

Predicted band size: 35 kDa

false

• WB

Supplier Data

Western blot - Anti-FLJ11506 antibody (AB241294)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-FLJ11506 antibody (ab241294) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 15 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 15 µg

Predicted band size: 35 kDa

false

Exposure time: 30s