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AB326875

Anti-FLT3 antibody [EPR31042-508]

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Rabbit Recombinant Monoclonal Flt3 / CD135 antibody. Suitable for Flow Cyt and reacts with Mouse, Human samples.
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Flow Cytometry - Anti-FLT3 antibody [EPR31042-508] (AB326875)
  • Flow Cyt

Lab

Flow Cytometry - Anti-FLT3 antibody [EPR31042-508] (AB326875)

Flow cytometric analysis of THP-1 (human monocytic leukemia monocyte, Right) and U-937 (human histiocytic lymphoma monocyte, Left) cells labelling with ab326875 at 1/500 dilution (0.1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Negative control : U-937.

Flow Cytometry - Anti-FLT3 antibody [EPR31042-508] (AB326875)
  • Flow Cyt

Lab

Flow Cytometry - Anti-FLT3 antibody [EPR31042-508] (AB326875)

Flow cytometric analysis of Mouse bone marrow cells labelling with ab326875 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with CD3 conjugated to Alexa Fluor®647.

Flow Cytometry - Anti-FLT3 antibody [EPR31042-508] (AB326875)
  • Flow Cyt

Lab

Flow Cytometry - Anti-FLT3 antibody [EPR31042-508] (AB326875)

Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Right) and B16-F10 (mouse skin melanoma cell, Left) cells labelling with ab326875 at 1/500 dilution (0.1ug) / Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Negative control : B16-F10.

Flow Cytometry - Anti-FLT3 antibody [EPR31042-508] (AB326875)
  • Flow Cyt

Lab

Flow Cytometry - Anti-FLT3 antibody [EPR31042-508] (AB326875)

Flow cytometric analysis of Mouse bone marrow cells labelling with ab326875 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with CD34 conjugated to Brilliant Violet 421.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR31042-508

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB32537

Anti-ERK1 antibody [Y72]

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  • 5
  • 4
Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Primary Antibodies

AB196515

Alexa Fluor® 647 Anti-CD19 antibody [EPR5906]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD19 antibody [EPR5906] (AB196515)

  • 5
  • 1
Primary Antibodies

AB195013

Alexa Fluor® 488 Anti-CD34 antibody [EP373Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD34 antibody [EP373Y] (AB195013)

  • 5
  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.
See full target information Flt3

대체 명칭 보기

CD135, Flk-2, Flt-3, Flt3, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase 2, Fms-like tyrosine kinase 3, Tyrosine-protein kinase receptor flk-2, FLK-2, FLT-3
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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