Anti-FLT3 antibody [EPR31042-551]

• WB

Lab

Western blot - Anti-FLT3 antibody [EPR31042-551] (AB326101)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

FLT3 is a glycoprotein of approximately 130-160 kDa and is detected as a 120 kDa band after treatment with the deglycosylation enzyme Peptide : N-glycosidase F (PNGase F).

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 28895560).

Samples are non-boiled as boiling may cause protein aggregation.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-FLT3 antibody [EPR31042-551] (ab326101) at 1/1000 dilution

Lane 1:

Untreated EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 48 µg

Lane 2:

EL4 lysate treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130-160 kDa,120 kDa,124 kDa

true

Exposure time: 180s

• WB

Lab

Western blot - Anti-FLT3 antibody [EPR31042-551] (AB326101)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : heart, kidney (PMID : 18031378), B16-F10, Neuro-2a, F9.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 28895560).

Samples are non-boiled as boiling may cause protein aggregation.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa).

All lanes:

Western blot - Anti-FLT3 antibody [EPR31042-551] (ab326101) at 1/1000 dilution

Lane 1:

Mouse brain membrane fraction at 48 µg

Lane 2:

Mouse thymus membrane fraction at 48 µg

Lane 3:

Mouse heart membrane fraction at 48 µg

Lane 4:

Mouse kidney membrane fraction at 48 µg

Lane 5:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 48 µg

Lane 6:

B16-F0 (mouse melanoma epithelial-like cell) whole cell lysate at 48 µg

Lane 7:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 48 µg

Lane 8:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130-160 kDa,124 kDa

true

Exposure time: 180s

• WB

Lab

Western blot - Anti-FLT3 antibody [EPR31042-551] (AB326101)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 28895560).

Samples are non-boiled as boiling may cause protein aggregation.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa); Anti-Integrin alpha V antibody [EPR16800](1 : 5000)(125/135KDa).

All lanes:

Western blot - Anti-FLT3 antibody [EPR31042-551] (ab326101) at 1/1000 dilution

Lane 1:

Mouse brain membrane fraction at 48 µg

Lane 2:

Mouse brain total tissue lysate at 48 µg

Lane 3:

Mouse thymus membrane fraction at 48 µg

Lane 4:

Mouse thymus total tissue lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130-160 kDa,124 kDa,125-135 kDa

true

Exposure time: 180s