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AB323595

Anti-Flt3 / CD135 Antibody [YCA-26627-SBC-12] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Flt3 / CD135 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
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Sandwich ELISA - Anti-Flt3 / CD135 Antibody [YCA-26627-SBC-12] - BSA and Azide free (Detector) (AB323595)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Flt3 / CD135 Antibody [YCA-26627-SBC-12] - BSA and Azide free (Detector) (AB323595)

Sandwich ELISA of ab323592 with the capture antibody ab323596 at 2 μg/mL and detector antibody ab323595 at 0.5 μg/mL. Background-subtracted absorbance data values (mean +/- standard deviation) are displayed.

Sandwich ELISA - Anti-Flt3 / CD135 Antibody [YCA-26627-SBC-12] - BSA and Azide free (Detector) (AB323595)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Flt3 / CD135 Antibody [YCA-26627-SBC-12] - BSA and Azide free (Detector) (AB323595)

Interpolated concentration of FLT3 / CD135 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : THP-1 cell extract 15.625 µg/ml and HeLa cell extract 15.625 µg/ml. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- standard deviation, n=2). Sample dilutions are made in 1% Bovine Serum Albumin (BSA). In the HeLa cell extract the signal was non-detectable (ND). Data represented here are indicative of assay working using example of biological samples. However, we do not guarantee same sensitivity level due to biological variation.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

YCA-26627-SBC-12

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB275551

Mouse Flt3 ligand ELISA Kit (Flt3L)

Recombinant|SimpleStep

Sandwich ELISA - Mouse Flt3 ligand ELISA Kit (Flt3L) (AB275551)

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Primary Antibodies

AB249687

Anti-Flt3 / CD135 (phospho Y589) antibody [EPR2311(2)] - BSA and Azide free

RabMAb|Recombinant

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Primary Antibodies

AB52648

Anti-Flt3 ligand/Flt3L antibody [EP1140Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Flt3 ligand/Flt3L antibody [EP1140Y] (AB52648)

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Primary Antibodies

AB227658

Anti-Ras (mutated Q61R) antibody [SP174]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ras (mutated Q61R) antibody [SP174] (AB227658)

  • 4
  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab323595 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Flt3 / CD135. The recommended pair for sandwich ELISA is:
Capture: ab323596, Human Flt3 / CD135 Capture Antibody (unconjugated)
Detector: ab323595, Human Flt3 / CD135 Detector Antibody (unconjugated)
The reference range value is 9.7-312 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purity
undefined Mass Spectrometry and HPLC
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.
See full target information FLT3

대체 명칭 보기

CD135, FLK2, STK1, FLT3, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase-2, Fms-like tyrosine kinase 3, Stem cell tyrosine kinase 1, FLK-2, FLT-3, STK-1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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