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AB60721

Anti-FOXA2 antibody [7E6] - BSA and Azide free

Anti-FOXA2 항체 [7E6] - BSA and Azide free

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(28 제품이 사용된 논문 )

Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting FOXA2 in Western Blot, Flow Cytometry, IP, ICC/IF. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Over 20 publications
- Trusted since 2007

대체 명칭 보기

HNF3B, TCF3B, FOXA2, Hepatocyte nuclear factor 3-beta, HNF-3-beta, HNF-3B, Forkhead box protein A2, Transcription factor 3B, TCF-3B

5 이미지
Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721)

ab60721 at 10 ug/ml staining FOXA2 in human Hella cells by Immunocytochemistry/ Immunofluorescence.

This image was generated using the ascites version of the product.

Flow Cytometry - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721)

Overlay histogram showing HepG2 cells stained with ab60721 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60721, 0.5μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

Western blot - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721)
  • WB

Lab

Western blot - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721)

Western blot : Anti-FOXA2 antibody [7E6]-BSA and Azide free ab60721 staining at 1 g/mL, shown in green; anti-GST antibody [EPR4236] ab111947 staining at 1/200 dilution, shown in magenta.. A band was observed at 78 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse 800CW and Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721) at 1 µg/mL

Lane 1:

FOXA1 Recombinant Protein (GST Tag) at 0.1 µg

Lane 2:

FOXA2 Recombinant Protein (GST Tag) at 0.1 µg

Lane 3:

FOXA3 Recombinant Protein (GST Tag) at 0.1 µg

Secondary

All lanes:

Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 78 kDa

Observed band size: 78 kDa

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Western blot - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721)
  • WB

Unknown

Western blot - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721) at 1 µg/mL

All lanes:

HepG2 cell lysate at 25 µg

Predicted band size: 48 kDa

Observed band size: 40 kDa,42 kDa,50 kDa

false

Western blot - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721)
  • WB

CiteAb

Western blot - Anti-FOXA2 antibody [7E6] - BSA and Azide free (AB60721)

Western Blotting using Anti-FOXA2 antibody [7E6] - BSA and Azide free, ab60721. Publication image from Cui, W. et al., 2015, Nat Commun, 25998442. Legend direct from paper.

Inhibition of mTOR enhances DE differentiation of hESCs.(a) qRT–PCR showing gene expression in hESCs treated with Activin A±Torin for 2 days. Data represent mean±s.d. of six measurements from two independent experiments. (b) Immunoblot showing protein expression in hESCs treated as in a. (c) Phase-contrast images of hESCs treated for 3 days with Activin A together with either LY or Torin. Scale bar, 100 µm. (d) Phase-contrast images of the cells from c that were further differentiated by a hepatic differentiation protocol as shown in Fig. 1. Days of differentiation are indicated. Scale bar, 50 µm.

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

7E6

Isotype

IgG2a

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, IP, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FOXA2 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y261

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대체품

Primary Antibodies

AB193880

HRP Anti-FOXA2 antibody [EPR4466]

RabMAb|Recombinant

Western blot - HRP Anti-FOXA2 antibody [EPR4466] (AB193880)

  • 0
  • 0
Primary Antibodies

AB108396

Anti-FOXA2 antibody [EPR4465] - ChIP Grade

RabMAb|Recombinant|Advanced Validation

Immunocytochemistry/ Immunofluorescence - Anti-FOXA2 antibody [EPR4465] - ChIP Grade (AB108396)

  • 0
  • 0
Primary Antibodies

AB108422

Anti-FOXA2 antibody [EPR4466]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] (AB108422)

  • 4
  • 12
Primary Antibodies

AB220810

Anti-FOXA2 antibody [EPR4466] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXA2 antibody [EPR4466] - BSA and Azide free (AB220810)

  • 0
  • 0
Primary Antibodies

AB193879

Alexa Fluor® 647 Anti-FOXA2 antibody [EPR4466]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-FOXA2 antibody [EPR4466] (AB193879)

  • 0
  • 2
Primary Antibodies

AB208376

Alexa Fluor® 488 Anti-FOXA2 antibody [EPR4465]

RabMAb|Recombinant

Flow Cytometry - Alexa Fluor® 488 Anti-FOXA2 antibody [EPR4465] (AB208376)

  • 0
  • 0
Primary Antibodies

AB193864

Alexa Fluor® 488 Anti-FOXA2 antibody [EPR4466]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-FOXA2 antibody [EPR4466] (AB193864)

  • 0
  • 0

보완 제품

Assay Kits

AB300315

Human FOXA2 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human FOXA2 ELISA Kit (AB300315)

  • 0
  • 0
Primary Antibodies

AB209665

Anti-Brachyury / Bry antibody [EPR18113]

RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brachyury / Bry antibody [EPR18113] (AB209665)

  • 5
  • 9
Primary Antibodies

AB224637

Anti-SOX17 antibody [EPR20684]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-SOX17 antibody [EPR20684] (AB224637)

  • 4
  • 6
Primary Antibodies

AB195045

Anti-PAX6 antibody [EPR15858]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] (AB195045)

  • 5
  • 9
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
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Reactivity 정보

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제품 세부 정보

What is this antibody validated in?
Anti-FOXA2 antibody [7E6] - BSA and Azide free (ab60721) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of FOXA2?
Anti-FOXA2 [7E6] - BSA and Azide free (ab60721) specifically detects a band for FOXA2 (UniProt: Q9Y261) at a molecular weight of 48kDa.

Trusted by the scientific community
Anti-FOXA2 [7E6] - BSA and Azide free (ab60721) was first used in a scientific publication in 2007 and has been cited over 20 times in peer-reviewed journals.
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
Purification 관련 사항
Purified from cell culture supernatant.
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FOXA2 also known as HNF3B is a transcription factor that plays a significant role in gene regulation. This protein has a mass of approximately 47 kDa and is expressed in various tissues including the liver pancreas and lung. Researchers detect FOXA2 using techniques like ELISA for protein quantification. Its function involves regulating the expression of genes critical for development and metabolism.
Biological function summary

FOXA2 influences important processes in embryonic development and organogenesis. It does not typically act as part of a larger complex but independently binds to DNA enabling the transcription of target genes. FOXA2 affects liver function by regulating genes involved in glucose and lipid metabolism and it also plays a part in the differentiation of cells in development stages.

Pathways

FOXA2 operates within pathways like the insulin signaling pathway and the Wnt signaling pathway. It collaborates with other transcription factors like FOXA1 and FOXA3 to maintain metabolic homeostasis. In the Wnt pathway FOXA2 impacts cell fate decisions essential for embryonic patterning and development.

Alterations in FOXA2 expression have connections to conditions such as diabetes and liver diseases. Changes in FOXA2 expression could disrupt liver metabolism leading to metabolic disorders. Moreover FOXA2 ties to the regulation of other proteins like PDX1 in the context of diabetes where it plays a role in insulin biosynthesis and pancreatic cell function.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
See full target information FOXA2
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제품이 사용된 논문 (28)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Stem cell research 77:103424 PubMed38677032

2024

Generation and characterization of six human induced pluripotent stem cell lines (hiPSCs) from three individuals with SSADH Deficiency and CRISPR-corrected isogenic controls.

Applications

Unspecified application

Species

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Wardiya Afshar-Saber,Cidi Chen,Nicole A Teaney,Kristina Kim,Ziqin Yang,Federico M Gasparoli,Darius Ebrahimi-Fakhari,Elizabeth D Buttermore,Ivy Pin-Fang Chen,Phillip L Pearl,Mustafa Sahin

iScience 26:108205 PubMed38026193

2023

Human pluripotent stem cell fate trajectories toward lung and hepatocyte progenitors.

Applications

Unspecified application

Species

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Chaido Ori,Meshal Ansari,Ilias Angelidis,Ruth Olmer,Ulrich Martin,Fabian J Theis,Herbert B Schiller,Micha Drukker

American journal of physiology. Lung cellular and molecular physiology 325:L689-L708 PubMed37642665

2023

Caveolin-1-derived peptide attenuates cigarette smoke-induced airway and alveolar epithelial injury.

Applications

Unspecified application

Species

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Durgesh Nandini Das,Bijesh Puthusseri,Venkadesaperumal Gopu,Venugopal Krishnan,Ashoka Kumar Bhagavath,Sudhir Bolla,Yogesh Saini,Gerald J Criner,Nathaniel Marchetti,Hua Tang,Nagarjun V Konduru,Liang Fan,Sreerama Shetty

Scientific data 10:93 PubMed36788249

2023

A dataset of definitive endoderm and hepatocyte differentiations from human induced pluripotent stem cells.

Applications

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Species

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Yuki Tanaka,Erina Furuhata,Shiori Maeda,Mami Kishima,Harukazu Suzuki,Takahiro Suzuki

eLife 11: PubMed36129169

2022

Contribution of -labeled cells to alveolar regeneration is independent of tuft cells.

Applications

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Species

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Huachao Huang,Yinshan Fang,Ming Jiang,Yihan Zhang,Jana Biermann,Johannes C Melms,Jennifer A Danielsson,Ying Yang,Li Qiang,Jia Liu,Yiwu Zhou,Manli Wang,Zhihong Hu,Timothy C Wang,Anjali Saqi,Jie Sun,Ichiro Matsumoto,Wellington V Cardoso,Charles W Emala,Jian Zhu,Benjamin Izar,Hongmei Mou,Jianwen Que

Stem cell research 64:102889 PubMed35961103

2022

Reprogramming of dermal fibroblasts from a Duchenne muscular dystrophy patient carrying a deletion of exons 45-50 into an induced pluripotent stem cell line (CCMi005-A).

Applications

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Species

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Davide Rovina,Elisa Castiglioni,Sara Mallia,Martina Rabino,Andrea Farini,Marzia Belicchi,Giusy Di Giuseppe,Cristina Gervasini,Yvan Torrente,Giulio Pompilio,Aoife Gowran

Stem cell research 61:102758 PubMed35364395

2022

Characterization of an induced pluripotent stem cell line (UMi040-A) bearing an auditory neuropathy spectrum disorder-associated variant in TMEM43.

Applications

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Species

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Pei-Ciao Tang,Marie V Roche,Se Young Um,Nicholas C Gosstola,Min Young Kim,Byung Yoon Choi,Derek M Dykxhoorn,Xue Zhong Liu

Frontiers in oncology 11:605025 PubMed33718155

2021

FOXA2-Interacting FOXP2 Prevents Epithelial-Mesenchymal Transition of Breast Cancer Cells by Stimulating E-Cadherin and PHF2 Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxiang Liu,Taolin Chen,Mingyue Guo,Yu Li,Qian Zhang,Guixiang Tan,Li Yu,Yongjun Tan

Journal of cellular and molecular medicine : PubMed32692488

2020

A functional genomics approach to investigate the differentiation of iPSCs into lung epithelium at air-liquid interface.

Applications

Unspecified application

Species

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Jenny L Kerschner,Alekh Paranjapye,Shiyi Yin,Dannielle L Skander,Gurkan Bebek,Shih-Hsing Leir,Ann Harris

Development, growth & differentiation 62:243-259 PubMed32277710

2020

Modeling early stages of endoderm development in epiblast stem cell aggregates with supply of extracellular matrices.

Applications

Unspecified application

Species

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Sachiko Inamori,Mai Fujii,Sayaka Satake,Hideaki Iida,Machiko Teramoto,Tomoyuki Sumi,Chikara Meno,Yasuo Ishii,Hisato Kondoh
제품이 사용된 논문 모두 보기
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