AB168383

Anti-FOXF1 antibody [EPR7971]

Anti-FOXF1 항체 [EPR7971]

RabMAb
Recombinant
각 태그의 뜻

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(7 제품이 사용된 논문 )

Anti-FOXF1 antibody [EPR7971] (ab168383) is a rabbit monoclonal antibody detecting FOXF1 in Western Blot, Flow Cytometry (Intra), ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

대체 명칭 보기

FKHL5, FREAC1, FOXF1, Forkhead box protein F1, Forkhead-related activator 1, Forkhead-related protein FKHL5, Forkhead-related transcription factor 1, FREAC-1

4 이미지

Immunocytochemistry/ Immunofluorescence - Anti-FOXF1 antibody [EPR7971] (AB168383)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FOXF1 antibody [EPR7971] (AB168383)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FOXF1 with purified ab168383 at 1 : 250 dilution (2.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cytometry (Intracellular) - Anti-FOXF1 antibody [EPR7971] (AB168383)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-FOXF1 antibody [EPR7971] (AB168383)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling FOXF1 with purified ab168383 at 1/70 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Lab

Western blot - Anti-FOXF1 antibody [EPR7971] (AB168383)

This antibody detects high background

All lanes:

Western blot - Anti-FOXF1 antibody [EPR7971] (ab168383) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

Human lung lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

false

Lab

Western blot - Anti-FOXF1 antibody [EPR7971] (AB168383)

We recommend using freshly made lysate in western blot testing to get stronger band and avoid high background.

All lanes:

Western blot - Anti-FOXF1 antibody [EPR7971] (ab168383) at 1/1000 dilution

Lane 1:

Frozen HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Fresh HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

false

Exposure time: 60s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7971

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/70 - 1/500", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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제품 세부 정보

What is this antibody validated in?
Anti-FOXF1 antibody [EPR7971] (ab168383) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of FOXF1?
Anti-FOXF1 [EPR7971] (ab168383) specifically detects a band for FOXF1 (UniProt: Q12946) at a molecular weight of 40kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR7971] also available for your convenience: ab168383, Alexa Fluor® 488 - ab214875, Alexa Fluor® 647 - ab215940, Carrier free - ab240153, APC - ab319331, PE - ab319468, Alexa Fluor® 555 - ab319965

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FOXF1 also known as Forkhead Box F1 is a transcription factor with a molecular mass of approximately 47 kDa. This protein belongs to the forkhead family which is characterized by a distinct forkhead DNA-binding domain. FOXF1 is expressed mainly in the mesenchyme of developing organs such as the lung and gut where it plays an essential role in organogenesis and development.

Biological function summary

FOXF1 regulates gene expression involved in cellular differentiation and embryonic development. It acts as a transcriptional activator binding specific DNA sequences to initiate transcription. FOXF1 does not function in isolation; it forms part of transcriptional complexes by interacting with other regulatory proteins enhancing its role as a transcription factor in specific tissue types. Through these interactions FOXF1 influences the development of organs and tissues.

Pathways

FOXF1 actively participates in the Wnt and Hedgehog signaling pathways both critical for embryonic development and cellular proliferation. In the Wnt pathway FOXF1 interacts with Β-catenin a central protein in the pathway to regulate target gene expression that controls cell differentiation and proliferation. In the Hedgehog pathway FOXF1 modulates gene expression alongside transcription factors such as GLI proteins controlling various developmental processes.

FOXF1 mutations or dysregulation have strong associations with congenital diaphragmatic hernia and alveolar capillary dysplasia both of which are severe developmental conditions. FOXF1 dysregulation disrupts normal lung and diaphragm development linking it to these disorders. Additionally the Hedgehog pathway where FOXF1 plays a role involves GLI proteins tying FOXF1 indirectly to certain developmental syndromes when mutations are present.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Probable transcription activator for a number of lung-specific genes.

See full target information FOXF1

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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology reports 50: PubMed37539708

2023

Forkhead box F1 functions as a novel prognostic biomarker and induces caspase‑dependent apoptosis in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yining Hao,Wei He,Haofei Wang,Wenbin Rui,Fukang Sun,Yu Zhu,Danfeng Xu,Chenghe Wang

Experimental and therapeutic medicine 25:103 PubMed36798677

2023

FOXF1 attenuates TGF‑β1‑induced bronchial epithelial cell injury by inhibiting CDH11‑mediated Wnt/β‑catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Chen,Xing Liao,Ling Lin,Ling Wu,Qiuyu Tang

Stem cells international 2021:9964159 PubMed34257670

2021

Adipose-Derived Stem Cell-Derived Extracellular Vesicles Inhibit the Fibrosis of Fibrotic Buccal Mucosal Fibroblasts via the MicroRNA-375/FOXF1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Han,Yanhui Zhang,Yuxia Xiao,Bohong Shi,Hong Wu,Desheng Liu

Methods in molecular biology (Clifton, N.J.) 2214:41-58 PubMed32944902

2020

Spatially Organized Differentiation of Mouse Pluripotent Stem Cells on Micropatterned Surfaces.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie M Morgani,Anna-Katerina Hadjantonakis

Cell reports 25:3215-3228.e9 PubMed30540952

2018

A Renewable Source of Human Beige Adipocytes for Development of Therapies to Treat Metabolic Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Su Su,Anyonya R Guntur,Daniel C Nguyen,Shameem S Fakory,Chad C Doucette,Cassandra Leech,Humphrey Lotana,Matthew Kelley,Jaspreet Kohli,Julieta Martino,Sunder Sims-Lucas,Lucy Liaw,Calvin Vary,Clifford J Rosen,Aaron C Brown

Genome biology 19:118 PubMed30119690

2018

Functional CRISPR screen identifies AP1-associated enhancer regulating FOXF1 to modulate oncogene-induced senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqi Han,Li Li,Alejandro Piñeiro Ugalde,Arieh Tal,Zohar Manber,Eric Pinto Barbera,Veronica Della Chiara,Ran Elkon,Reuven Agami

Cancer discovery 8:234-251 PubMed29162563

2017

FOXF1 Defines the Core-Regulatory Circuitry in Gastrointestinal Stromal Tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Leili Ran,Yuedan Chen,Jessica Sher,Elissa W P Wong,Devan Murphy,Jenny Q Zhang,Dan Li,Kemal Deniz,Inna Sirota,Zhen Cao,Shangqian Wang,Youxin Guan,Shipra Shukla,Katie Yang Li,Alan Chramiec,Yuanyuan Xie,Deyou Zheng,Richard P Koche,Cristina R Antonescu,Yu Chen,Ping Chi

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FOXF1 antibody [EPR7971]

Anti-FOXF1 항체 [EPR7971]

Immunocytochemistry/ Immunofluorescence - Anti-FOXF1 antibody [EPR7971] (AB168383)

Flow Cytometry (Intracellular) - Anti-FOXF1 antibody [EPR7971] (AB168383)

Western blot - Anti-FOXF1 antibody [EPR7971] (AB168383)

Western blot - Anti-FOXF1 antibody [EPR7971] (AB168383)

관련 conjugated 항체와 다양한 조성의 항체 (6)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (7)

Product promise