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AB51774

Anti-FOXG1 antibody [mAbcam 51774]

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Mouse Monoclonal FOXG1 antibody. Suitable for Flow Cyt (Intra), WB and reacts with Human samples. Cited in 1 publication.
2 이미지
Flow Cytometry (Intracellular) - Anti-FOXG1 antibody [mAbcam 51774] (AB51774)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-FOXG1 antibody [mAbcam 51774] (AB51774)

Overlay histogram showing SHSY-5Y cells stained with ab51774 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51774, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Western blot - Anti-FOXG1 antibody [mAbcam 51774] (AB51774)
  • WB

Project

Western blot - Anti-FOXG1 antibody [mAbcam 51774] (AB51774)

All lanes:

Western blot - Anti-FOXG1 antibody [mAbcam 51774] (ab51774) at 5 µg/mL

All lanes:

Human brain tissue lysate - total protein (<a href='/ko/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Secondary

All lanes:

Rabbit polyclonal to Mouse IgG - H&L (HRP) at 1/2000 dilution

Predicted band size: 52 kDa

Observed band size: 40 kDa,50 kDa

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주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam 51774

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB3394

Anti-FOXG1 antibody

Flow Cytometry - Anti-FOXG1 antibody (AB3394)

  • 1
  • 1
Primary Antibodies

AB196868

Anti-FOXG1 antibody [EPR18987]

20ul selling size|RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] (AB196868)

  • 4
  • 1
Primary Antibodies

AB18259

Anti-FOXG1 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody (AB18259)

  • 4
  • 14
Primary Antibodies

AB227888

Anti-FOXG1 antibody [EPR18987] - BSA and Azide free

RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXG1 antibody [EPR18987] - BSA and Azide free (AB227888)

  • 0
  • 0

보완 제품

Primary Antibodies

AB240636

Anti-Ctip2 antibody [EPR23120-25]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Ctip2 antibody [EPR23120-25] (AB240636)

  • 5
  • 1
Primary Antibodies

AB195045

Anti-PAX6 antibody [EPR15858]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] (AB195045)

  • 5
  • 9
Primary Antibodies

AB183032

Anti-TBR1 antibody [EPR8138(2)]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-TBR1 antibody [EPR8138(2)] (AB183032)

  • 5
  • 6
Primary Antibodies

AB319166

Anti-TBR2 / Eomes antibody [RM2055]

BOND RX™ Validated|20ul selling size|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR2 / Eomes antibody [RM2055] (AB319166)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.5-1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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제품 세부 정보

We can conjugate this antibody to FITC for you (please see ab150200 for details).

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FOXG1 also known as Brain Factor 1 (BF-1) is a transcription factor with a molecular weight of approximately 52 kDa. This protein plays an important role in the modulation of gene expression. FOXG1 is found mainly in the developing brain and other tissues of the central nervous system. In adult brains its expression levels significantly decrease although some residual expression persists in areas such as the hippocampus.
Biological function summary

FOXG1 regulates processes of neural development and brain function. It is not known to be part of a protein complex but FOXG1 binds to DNA influencing the transcriptional activity of genes involved in neurogenesis. The protein contributes to the development of structures such as the telencephalon and regulates transitions between different stages of neuronal cell development. Its role extends to maintaining the balance between proliferation and differentiation of neural progenitor cells.

Pathways

FOXG1 participates in the Notch and Wnt signaling pathways. These pathways are critical for cell fate determination and embryonic development. FOXG1 interacts with proteins like those in the Notch pathway to maintain neural progenitors in a proliferative state. In the Wnt signaling pathway FOXG1 helps in modulating the signaling that controls brain patterning working in concert with other transcription factors and signaling molecules.

FOXG1 mutations can lead to congenital conditions such as FOXG1 syndrome and Rett syndrome. In such disorders abnormalities in FOXG1 disrupt normal neural development leading to cognitive and developmental impairments. The involvement of FOXG1 with the MeCP2 protein in Rett syndrome highlights its importance in maintaining normal neurological function. Understanding FOXG1's interactions and functions provides insights into potential therapeutic approaches for these disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon.
See full target information FOXG1

대체 명칭 보기

FKH2, FKHL1, FKHL2, FKHL3, FKHL4, FOXG1A, FOXG1B, FOXG1C, FOXG1, Forkhead box protein G1, Brain factor 1, Brain factor 2, Forkhead box protein G1A, Forkhead box protein G1B, Forkhead box protein G1C, Forkhead-related protein FKHL1, Forkhead-related protein FKHL2, Forkhead-related protein FKHL3, BF-1, BF1, BF-2, BF2, hBF-2, HFK1, HFK2, HFK3
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 16:1 PubMed39753531

2025

Endothelial-Ercc1 DNA repair deficiency provokes blood-brain barrier dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Cathrin E Hansen,Davide Vacondio,Lennart van der Molen,Annika A Jüttner,Wing Ka Fung,Manon Karsten,Bert van Het Hof,Ruud D Fontijn,Gijs Kooij,Maarten E Witte,Anton J M Roks,Helga E de Vries,Inge Mulder,Nienke M de Wit
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

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