Anti-FOXP1 antibody [EPR4113]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FOXP1 antibody [EPR4113] (AB134055)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling FOXP1 with purified ab134055 at 1/500 dilution (0.4 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-FOXP1 antibody [EPR4113] (AB134055)

Intracellular Flow Cytometry analysis of Daudi (human Burkitt's lymphoma) cells labeling FOXP1 with purified ab134055 at 1/200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP1 antibody [EPR4113] (AB134055)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human diffuse large B-cell lymphoma tissue sections labeling FOXP1 with purified ab134055 at 1/1000 dilution (0.21 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• ChIP-seq

Lab

ChIP-sequencing - Anti-FOXP1 antibody [EPR4113] (AB134055)

Chromatin was prepared from K-562 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab134055 [EPR4113]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.

• ChIP-seq

Lab

ChIP-sequencing - Anti-FOXP1 antibody [EPR4113] (AB134055)

Chromatin was prepared from K-562 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab134055 [EPR4113]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.

• ChIP-seq

Lab

ChIP-sequencing - Anti-FOXP1 antibody [EPR4113] (AB134055)

Chromatin was prepared from K-562 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10⁷ cells and 8 µg of ab134055 [EPR4113]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.

• ChIP-seq

Lab

ChIP-sequencing - Anti-FOXP1 antibody [EPR4113] (AB134055)

Chromatin was prepared from MEF cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5 x 10^6 cells and 4 µg of ab134055 [EPR4113]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• ChIP-seq

Lab

ChIP-sequencing - Anti-FOXP1 antibody [EPR4113] (AB134055)

Chromatin was prepared from MEF cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5 x 10^6 cells and 4 µg of ab134055 [EPR4113]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• ChIP-seq

Lab

ChIP-sequencing - Anti-FOXP1 antibody [EPR4113] (AB134055)

Chromatin was prepared from MEF cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5 x 10^6 cells and 4 µg of ab134055 [EPR4113]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

• WB

Lab

Western blot - Anti-FOXP1 antibody [EPR4113] (AB134055)

The multiple bands are consistent with what have been described in the literature PMID : 12692134.

All lanes:

Western blot - Anti-FOXP1 antibody [EPR4113] (ab134055) at 1/1000 dilution

Lane 1:

Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysates at 20 µg

Lane 2:

RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

Lane 3:

Mouse lung cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 75 kDa

Observed band size: 50 kDa,70 kDa,95 kDa

false

• WB

Lab

Western blot - Anti-FOXP1 antibody [EPR4113] (AB134055)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

Lysate was freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-FOXP1 antibody [EPR4113] (ab134055) at 1/1000 dilution

Lane 1:

HEK 293 (human embryonic kidney epithelial cell) whole cell lysate (Frozen) at 20 µg

Lane 2:

HEK 293 (human embryonic kidney epithelial cell) whole cell lysate (Fresh) at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Observed band size: 90 kDa,70 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-FOXP1 antibody [EPR4113] (AB134055)

The multiple bands are consistent with what have been described in the literature PMID : 12692134.

All lanes:

Western blot - Anti-FOXP1 antibody [EPR4113] (ab134055) at 1/5000 dilution

All lanes:

2.4G2 (Rat B cell lymphoma B lymphocyte) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 75 kDa

Observed band size: 50 kDa,70 kDa,95 kDa

false