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AB243890

Anti-FOXP3 antibody [BLR034F] - BSA free

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal FOXP3 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human FOXP3 aa 250 to C-terminus.

대체 명칭 보기

IPEX, JM2, FOXP3, Forkhead box protein P3, Scurfin

5 이미지
Immunocytochemistry - Anti-FOXP3 antibody [BLR034F] - BSA free (AB243890)
  • ICC

Supplier Data

Immunocytochemistry - Anti-FOXP3 antibody [BLR034F] - BSA free (AB243890)

Formalin-fixed, paraffin-embedded MJ cells labeling FOXP3 using ab243890 in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (AB243890)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (AB243890)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for FOXP3 using ab243890 in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (AB243890)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [BLR034F] - BSA free (AB243890)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for FOXP3 using ab243890 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

Western blot - Anti-FOXP3 antibody [BLR034F] - BSA free (AB243890)
  • WB

Supplier Data

Western blot - Anti-FOXP3 antibody [BLR034F] - BSA free (AB243890)

Detection : Chemiluminesence

All lanes:

Western blot - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 50 µg

Lane 3:

MJ whole cell lysate at 50 µg

Lane 4:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 50 µg

Lane 5:

RPMI-8226 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 47 kDa

false

Exposure time: 30s

Immunoprecipitation - Anti-FOXP3 antibody [BLR034F] - BSA free (AB243890)
  • IP

Supplier Data

Immunoprecipitation - Anti-FOXP3 antibody [BLR034F] - BSA free (AB243890)

All lanes:

Immunoprecipitation - Anti-FOXP3 antibody [BLR034F] - BSA free (ab243890) at 1/1000 dilution

Lane 1:

MJ whole cell lysate at 20 %

Lane 2:

Control IgG in MJ whole cell lysate

Predicted band size: 47 kDa

false

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR034F

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC, IP

applications

Immunogen

Synthetic Peptide within Human FOXP3 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BZS1

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대체품

Primary Antibodies

AB22510

Anti-FOXP3 antibody [mAbcam 22510]

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] (AB22510)

  • 3
  • 8
Primary Antibodies

AB188638

Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [mAbcam 22510] - BSA and Azide free (AB188638)

  • 0
  • 0
Primary Antibodies

AB275080

Alexa Fluor® 594 Anti-FOXP3 antibody [SP97]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-FOXP3 antibody [SP97] (AB275080)

  • 0
  • 0
Primary Antibodies

AB275079

Alexa Fluor® 647 Anti-FOXP3 antibody [SP97]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-FOXP3 antibody [SP97] (AB275079)

  • 0
  • 0
Primary Antibodies

AB215206

Anti-FOXP3 antibody [EPR22102-37]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] (AB215206)

  • 5
  • 5
Primary Antibodies

AB244242

Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free

Recombinant|RabMAb

Flow Cytometry (Intracellular) - Anti-FOXP3 antibody [EPR22102-37] - BSA and Azide free (AB244242)

  • 4
  • 1
Primary Antibodies

AB99963

Anti-FOXP3 antibody [SP97]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [SP97] (AB99963)

  • 3
  • 3
Primary Antibodies

AB191416

Anti-FOXP3 antibody [EPR15038-69]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR15038-69] (AB191416)

  • 0
  • 0
Primary Antibodies

AB238809

Anti-FOXP3 antibody [SP97] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [SP97] - BSA and Azide free (AB238809)

  • 0
  • 0
Primary Antibodies

AB216000

Anti-FOXP3 antibody [EPR15038-69] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXP3 antibody [EPR15038-69] - BSA and Azide free (AB216000)

  • 5
  • 1

보완 제품

Assay Kits

AB289644

Human FOXP3 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human FOXP3 ELISA Kit (AB289644)

  • 0
  • 0
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>Use 6μl/mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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특성 및 보관 정보

제형
Liquid
Purification 관련 사항
Recombinant antibody was purified from cell culture supernatant.
보관 버퍼
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FOXP3 also recognized as Forkhead box P3 is a transcription factor critical for immune system function. It weighs approximately 47 kDa. FOXP3 is mainly expressed in regulatory T cells (Tregs) which play a role in maintaining immune tolerance. Scientists primarily identify its presence in the nucleus where it facilitates transcriptional regulation. Methods like 'FOXP3 IHC' and 'FOXP3 IHC mouse' are useful for detecting this protein in tissue samples while 'FOXP3 antibodies' aid in research on this target. Techniques like 'FOXP3 staining' and 'FOXP3 flow cytometry' further allow for the analysis of its expression and function.
Biological function summary

This transcription factor operates by regulating the expression of a variety of genes involved in Treg cell development and function. FOXP3 can interact with other proteins to form complexes that modulate gene expression. Its role is essential in enabling Tregs to suppress immune responses a process necessary for preventing autoimmunity. The interaction extends to other key regulators within Tregs such as the protein complex 221D which may have overlapping functional responsibilities. 'FOXP3 236a/E7' reflects its genetic variants detectable in different studies providing insights into its biological functions.

Pathways

FOXP3 is a significant player within the immune regulation pathway. It integrates into the TGF-beta signaling pathway in which it controls the expression of genes important for Treg functions. The protein recruits other transcription factors like NF-kB coordinating intricate network regulations within immune cells. FOXP3's activity orchestrates the balance between immune activation and suppression ensuring a well-functioning immune response that prevents overreactions leading to autoimmune conditions.

FOXP3 mutations or dysregulation can link to autoimmune diseases like immune dysregulation polyendocrinopathy enteropathy X-linked syndrome (IPEX). This transcription factor's malfunction implicates cancer where inadequate Treg function can either suppress antitumor immunity or result in tumor progression. Proteins such as CTLA-4 which is also involved in immune regulation connect to FOXP3 in these contexts emphasizing FOXP3’s significant role in maintaining immune homeostasis and its impact on disease development.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg) (PubMed : 17377532, PubMed : 21458306, PubMed : 23947341, PubMed : 24354325, PubMed : 24722479, PubMed : 24835996, PubMed : 30513302, PubMed : 32644293). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells (PubMed : 23169781). Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases (PubMed : 17377532, PubMed : 21458306, PubMed : 23947341, PubMed : 24354325, PubMed : 24722479). The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG) (PubMed : 17377532, PubMed : 21458306, PubMed : 23947341, PubMed : 24354325, PubMed : 24722479). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed : 15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed : 17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed : 17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed : 18368049). Inhibits the transcriptional activator activity of RORA (PubMed : 18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (By similarity).
See full target information FOXP3
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in immunology 16:1559278 PubMed40703517

2025

Prognostic significance and immune infiltration analysis of HMGA2 in endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Jiang,Jiaxin Yu,Yunfeng Zheng,Chenfan Tian,Yuan Tu,Chunxia Gong,Hangkun Yu,Yi Luo,Zhuoying Hu

Neoplasma 70:229-239 PubMed36964720

2023

High expression of WTAP is related to poor prognosis in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Juan Tao,Peng Zhang,Yuan-Yuan Chen,Ming Chen
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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