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AB325855

Anti-Frizzled-4 antibody [HL2824] - BSA and Azide free

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Rabbit Recombinant Monoclonal Frizzled 4 antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Zebrafish, Human samples. Immunogen corresponding to Synthetic Peptide within Human Frizzled-4.
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Western blot - Anti-Frizzled-4 antibody [HL2824] - BSA and Azide free (AB325855)
  • WB

Supplier Data

Western blot - Anti-Frizzled-4 antibody [HL2824] - BSA and Azide free (AB325855)

Unboiled various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325855 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Frizzled-4 antibody [HL2824] - BSA and Azide free (ab325855) at 1/1000 dilution

Lane 1:

Unboiled HepG2 whole cell extracts at 30 µg

Lane 2:

Unboiled Jurkat whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2824

Isotype

IgG

Carrier free

Yes

Reacts with

Zebrafish, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human Frizzled-4.

Q9ULV1

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보완 제품

Primary Antibodies

AB32572

Anti-beta Catenin antibody [E247] - ChIP Grade

BOND RX™ Validated|Advanced Validation|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody [E247] - ChIP Grade (AB32572)

  • 5
  • 75
Primary Antibodies

AB227229

Anti-Wnt5a antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt5a antibody (AB227229)

  • 0
  • 0
Primary Antibodies

AB186430

Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2]

RabMAb|Recombinant|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-Elav-type RNA-binding protein ETR3 antibody [EPR13374-2] (AB186430)

  • 5
  • 2
Primary Antibodies

AB219412

Anti-Wnt3a antibody [EPR21889]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] (AB219412)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Receptor for Wnt proteins (PubMed : 30135577). Most frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes (PubMed : 30135577). Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP) (By similarity). In retina, it can be activated by Wnt protein-binding and also by Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs (By similarity). A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
See full target information Frizzled-4

대체 명칭 보기

CD344, Frizzled-4, Fz-4, hFz4, FzE4, FZD4
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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